Rabbit anti parp

Mouse anti-Bax, mouse anti-Bcl-2, rabbit anti-Caspase-3, rabbit anti-Bid, rabbit anti-PARP, rabbit anti-Survivin, and rabbit anti-GAPDH were obtained from Proteintech (USA). Horseradish peroxidase- (HRP-) conjugated goat anti-mouse and anti-rabbit IgG antibodies were provided by Beijing Zhongshan Golden Bridge Biotechnology Co. Ltd. (Beijing, China). Protein assay kit was obtained from Beijing Pulilai Gene Technology Co., Ltd. (Beijing, China). Reverse transcription kit was obtained from Promega (USA).

The BCa cells and tumor tissue samples lysates were extracted with RIPA cell lysis buffer, and the protein concentration in the lysates was quantified using an enhanced bicinchoninic acid protein assay kit (Thermo Fisher Scientific, MA, USA) with bovine serum albumin as a standard. The total protein extract will be used for western blot analysis. Equal amounts of total protein of tissues or cells were subjected to 10% SDS-PAGE and transferred to a PVDF membrane followed by immunoblotting using the following primary polyclonal antibodies: mouse anti-TMEM40 (Santa Cruz, CA, USA), rabbit anti-p53 (Proteintech Group, INC, USA), rabbit anti-p21 (Proteintech Group, INC, USA), mouse anti-CCND1 (Proteintech Group, INC, USA), rabbit anti-Caspase-3 (Cell Signaling Technology, MA, USA), rabbit anti-Caspase-9 (Proteintech Group, INC, USA), rabbit anti-PARP (Proteintech Group, INC, USA), rabbit anti-c-MYC (Proteintech Group, INC, USA) and GAPDH (Cell Signaling Technology, MA, USA). Specific proteins were detected with enhanced chemiluminescence (ECL, Millipore, USA). Band density was measured (ImageJ software) and normalized to GAPDH.