Ammonium peroxodisulfate

Sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate as components of phosphate buffered saline (PBS) pH 7.4 were purchased from AppliChem (Darmstadt, Germany). A PAA gel developed by Loch et al. [16 (link)] simulating the vitreous body was prepared according to the composition given in Table 1. Rotiphoresis gel 30 (37.5:1), ammonium peroxodisulfate, and tetramethylethylenediamine were purchased from Carl Roth (Karlsruhe, Germany). Agarose for the PAA gel-sheath was purchased from Sigma Aldrich, Germany. Model implants were prepared via hot-melt extrusion of FS (Sigma Aldrich, St. Louis, MO, USA) or TA (Caelo, Hilden, Germany) as active pharmaceutical ingredients, hydroxypropyl methylcellulose (HPMC; Affinisol 100LV/Affinisol 15LV; Dow Chemicals, Midland, TX, USA) and polyethylene glycol (PEG) 6000 (Carl Roth). All chemicals and solvents for the high-performance liquid chromatography (HPLC) were of analytical quality. A standard Formlabs Clear Resin (Formlabs, Somerville, MA, USA) was used for the 3D-printed EFC.

Tris, urea, thiourea, CHAPS, dithiothreitol, bromophenol blue, glycerin, sodium dodecyl sulfate, glycine, temed, ammoniumperoxodisulfate, ammonium sulfate, ammonium bicarbonate, colloidal Coomassie Blue, and acrylamide were purchased from Roth (Karlsruhe, Germany). Iodacetamide was obtained from SERVA (Heidelberg, Germany) and benzonase was purchased from Novagen (Darmstadt, Germany). Ampholytes (Biolyte 3–10) were purchased from Bio-Rad Laboratories (München, Germany) and DeStreak was obtained from Amersham Bioscience (Freiburg, Germany).

Aniline (Sigma-Aldrich, Saint Louis, MO, USA) was distilled in vacuo before use. Na₂HPO₄‧2H₂O (Riedel-de Haën, Seelze, Germany), 2,2-azino-bis(3-ethyl-benzthiazoline-6-sulfonate (ABTS), citric acid anhydrous, sodium dodecylbenzenesulfonate (DBSNa), potassium octacyanomolybdate (IV) hydrate (Sigma-Aldrich, Steinheim, Germany), ammonium peroxodisulfate (Carl Roth GmbH, Karlsruhe, Germany), and tetrahydrofurane (Lab-Scan, Gliwice, Poland) were used without further purification. All the solutions were prepared using water purified with a Simplicity^® Water Purification System (Merck KGaA, Darmstadt, Germany).