NaCl, KCl, Na2HPO4·12H2O, KH2PO4, K3[Fe (CN)6], and K4[Fe (CN)6]·3H2O were purchased from Sinopharm Chemical Reagent Co., Ltd.; nitrocellulose filter membranes were purchased from Biosharp Co., Ltd.; and human breast cancer cells (MCF-7) were purchased from Life Science Institute of Zhejiang University. All chemicals used in this work were analytical grade and used as received. All aqueous solutions were prepared using ultrapure water (ultrapure water produced by Aquelix 5).
Na2hpo4 12h2o
Manufactured by Sinopharm
Sourced in China
Na2HPO4·12H2O is a chemical compound that consists of disodium hydrogen phosphate and twelve water molecules. It is a crystalline solid that is commonly used as a buffer solution in various laboratory and industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Sinopharm (15 mentions)
Nah2po4 2h2o, by Sinopharm (13 mentions)
Potassium chloride (kcl), by Sinopharm (10 mentions)
Kh2po4, by Sinopharm (7 mentions)
Sodium hydroxide (naoh), by Sinopharm (7 mentions)
Hydrochloric acid (hcl), by Sinopharm (5 mentions)
Ethanol, by Sinopharm (4 mentions)
N n n n tetramethylethylenediamine, by Sinopharm (2 mentions)
Tris hcl, by Sinopharm (2 mentions)
Acetic acid, by Sinopharm (2 mentions)
Tween 20, by Sinopharm (2 mentions)
Formic acid, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Sangon (2 mentions)
Potassium ferricyanide, by Sinopharm (2 mentions)
Cacl2, by Sinopharm (2 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (2 mentions)
Citric acid, by Sinopharm (2 mentions)
Nah2po4, by Sinopharm (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
K3fe cn 6, by Sinopharm (1 mentions)
33 protocols using na2hpo4 12h2o
1
Breast Cancer Cell Culture Protocol
2
Bioactive Compounds Extraction Optimization
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The fresh Lentinus edodes, Grifola frondosa, and Hericium erinaceus were purchased from the Gouzhuang Farmers' Market in Hangzhou and pulverized into powder. Ganoderma lucidum extract residue was provided by Zhejiang WisePlus Health Technology Corporation Ltd. NaOH, HCl, Na2HPO4·12H2O, NaHPO4·H2O, sodium taurocholate, sodium carboxymethyl cellulose, DNS, and soluble starch were purchased from Sinopharm Chemical Reagent Corporation Ltd. (Shanghai, China). Neutral protease, alpha amylase, and lipase were purchased from Beijing Gao Ruisen Technology Corporation Ltd. Corn oil was purchased from Shanghai Maclean Reagent Corporation Ltd. All the other chemicals used in this study were of analytical grade.
3
Characterization of Shrimp Spoilage Bacteria
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The shrimp (L. vannamei) was bought from Qidong Rd. Market, Qingdao, with a weight of 45 ± 5 g. The shrimp was transported to the laboratory on ice within 30 min.
The wild strain of L. plantarum SS-128 and the luxS-mutant strain of L. plantarum SS-128 (ΔluxS) were constructed and preserved by the Laboratory of Aquatic Products Higher Application Technology, Ocean University of China. Shewanella baltica is a laboratory-preserved strain previously isolated from spoiled L. vannamei (Zhu et al., 2015 (link)). DPD was obtained from Professor Ming Li’s lab in the College of Pharmacy, Ocean University of China. The De Man, Rogosa and Sharpe (MRS) broth, Lactobacillus medium, and agar were bought from Qingdao Haibo Biotechnology Co., Ltd., China. Na2HPO4⋅12H2O, NaH2POx⋅H2O, NaCl, 40% Acr-Bis, Tris–HCl, N,N,N′,N′-tetramethylethylenediamine, Tris, dithiothreitol, and other analytical pure chemical reagents were purchased from Sinopharm Group Chemical Reagent Co., Ltd.
The wild strain of L. plantarum SS-128 and the luxS-mutant strain of L. plantarum SS-128 (ΔluxS) were constructed and preserved by the Laboratory of Aquatic Products Higher Application Technology, Ocean University of China. Shewanella baltica is a laboratory-preserved strain previously isolated from spoiled L. vannamei (Zhu et al., 2015 (link)). DPD was obtained from Professor Ming Li’s lab in the College of Pharmacy, Ocean University of China. The De Man, Rogosa and Sharpe (MRS) broth, Lactobacillus medium, and agar were bought from Qingdao Haibo Biotechnology Co., Ltd., China. Na2HPO4⋅12H2O, NaH2POx⋅H2O, NaCl, 40% Acr-Bis, Tris–HCl, N,N,N′,N′-tetramethylethylenediamine, Tris, dithiothreitol, and other analytical pure chemical reagents were purchased from Sinopharm Group Chemical Reagent Co., Ltd.
4
Synthesis and Characterization of Hyaluronic Acid
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The high-molecular-weight HA sample (2000 kDa) and low-molecular-weight sample (200 kDa) were purchased from Bloomage Freda Biopharm Co. Ltd. (Shandong, China); 1,4-butanediol diglycidyl ether (BDDE), (methyl sulfoxide)-d6 and d -glucuronic acid were purchased from Meryer (Shanghai) Chemical Technology Co., Ltd. (Shanghai, China); NaOH, KOH, NaH2PO4·2H2O, Na2HPO4·12H2O, sodium tetraborate decahydrate and nicotinamide were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China); hyaluronidase was purchased from Shanghai yuanye Bio-Technology Co. Ltd. (Shanghai, China); H2SO4 was purchased from Shanghai Lingfeng Chemical Reagent Co. Ltd (Shanghai, China); carbazole and formic acid were purchased from Shanghai Aladdin Bio-Chem Technology Co. Ltd (Shanghai, China); acetophenone was purchased from Energy Chemical (Shanghai, China). All the chemicals were of analytical grade.
5
Western Blot Analysis of BAX, Ki67, VEGF
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The following commercially available antibodies were used: anti-BAX (Servicebio, cat: GB11007-1, 1:50 dilution, Wuhan, China), anti-Ki67 (Abcam, cat: Ab16667, 1:200 dilution, Cambridge, MA, USA), anti-VEGF (Servicebio, cat: GB11007-1, 1:100 dilution, Wuhan, China).
The following commercially available reagents were also used: SDS sample buffer (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China, cat: 30166428), 30% acrylamide (Biosharp, cat: BL513b, Hefei, China), Trise-Base (Biofavor biotech, cat: 1115GR500, Wuhan, China), Glycine (Biofavor biotech, cat: 1275GR500, Wuhan, China), methyl alcohol, NaCl, KCl, Na2HPO4.12H2O, KH2PO4, acetic acid, Tween-20 (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China), protein markers (Thermo Fisher Scientific, Dover, DE, USA).
The following commercially available reagents were also used: SDS sample buffer (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China, cat: 30166428), 30% acrylamide (Biosharp, cat: BL513b, Hefei, China), Trise-Base (Biofavor biotech, cat: 1115GR500, Wuhan, China), Glycine (Biofavor biotech, cat: 1275GR500, Wuhan, China), methyl alcohol, NaCl, KCl, Na2HPO4.12H2O, KH2PO4, acetic acid, Tween-20 (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China), protein markers (Thermo Fisher Scientific, Dover, DE, USA).
6
HPLC-MS/MS-based Targeted Metabolomics
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HPLC grade methanol and acetonitrile were purchased from TEDIA (Shanghai, China) and Sigma-Aldrich (Shanghai, China), respectively. Na2HPO4·12H2O and NaH2PO4·2H2O, boric acid, sodium hydroxide, ethylenediaminetetraacetic acid (EDTA), dimethylsulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China) all as analytical grade reagents. Formic acid, ascorbic acid, tris(2-carboxyethyl)phosphinehydrochloride (TCEP), N,N’-disuccinimidyl carbonate (DSC), N-ethylmaleimide (NEM), 4-tert-butylbenzenethiol (tBBT), 5-aminoisoquinoline (5-AIQ) and 6-aminoquinoline (6-AQ) were purchased from Sigma-Aldrich (Shanghai, China) together with 126 analyte standards used here. (see details in Table 1 ).
7
Synthesis of Silver Nanoparticles
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Silver nitrate, polyvinyl pyrrolidone, melamine, and the experimental reagents (Na2HPO4·12H2O and MO) were provided by Sinopharm Chemical Reagent Co., Shanghai Branch, Shanghai, China [44 (link)]. All experimental reagents used were analytically pure and solutions were prepared with ultrapure or deionized water.
8
Electrochemical Aptasensor for Pesticide Detection
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Tris (2,2′-bipyridyl) ruthenium (II) chloride hexahydrate and Gold (III) chloride trihydrate (HAuCl4⋅3H2O) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). MWCNTs (>95 %, purity), polyethyleneimine, 1-Hexanol, cyclohexane, NH4OH, anhydrous ethanol, acetone, KCl, NaCl, NaH2PO4⋅2H2O, Na2HPO4⋅12H2O, trisodium citrate dihydrate, potassium ferricyanide were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Profenofos, malathion, chlorpyrifos and acetamiprid were purchased from Beijing Putian Tongchuang Biotechnology Co., Ltd. (Beijing, China).
The aptamer sequence selected with reference to this document was modified with sulfhydryl [40 (link)]. The sequence of aptamer was 5-(SH)–(CH2)6–AAGCTTTTTTGACTGCAGGTGAAAAAGAG-3′. Aptamer, 1 × TE buff and bovine serum albumin (BSA) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China).
The aptamer sequence selected with reference to this document was modified with sulfhydryl [40 (link)]. The sequence of aptamer was 5-(SH)–(CH2)6–AAGCTTTTTTGACTGCAGGTGAAAAAGAG-3′. Aptamer, 1 × TE buff and bovine serum albumin (BSA) were purchased from Sangon Biotech Co., Ltd. (Shanghai, China).
9
Vatalanib Dihydrochloride for Therapeutic Use
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The vatalanib dihydrochloride PTK787 was obtained from Selleck Chemicals (Shanghai, China). Methyl sulfoxide, HCl, glycerin, sodium dodecyl sulfate, NaCl, KCl, Na2HPO4·12H2O, and KH2PO4 were purchased from Sinopharm (Shanghai, China). XueSaiTong (XST) was obtained from TeAnNa (Yunnan, China). Trizol was obtained from Invitrogen (Wuhan, China). Analytical-grade methanol, acetonitrile, and formic acid were purchased from Merck (Darmstadt, Germany).
10
Electrochemical Sensing of Biomolecules
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Au target (99.99% purity, size 10 mm × 10 mm × 1.0 mm) was obtained from BAS. Co., Ltd. AA, UA, AP, ethanol, Na2HPO4•12H2O, NaH2PO4•3H2O, concentrated sulfuric acid (H2SO4) and glucose were obtained from Sinopharm Chemical Regent Co., Ltd. All of the chemicals were of analytical grade. Phosphate buffer solution (PBS, 0.1 M, pH 7.4) was made up from Na2HPO4•12H2O and NaH2PO4•3H2O.
Solutions of glucose, AA, UA and AP were prepared using PBS before the electrochemical test. Glassy carbon plates (size 10 mm × 10 mm × 1.0 mm) and gold bulk electrodes (diameter, 2.0 mm) were ordered from BAS Co., Ltd. The GC plates were polished sequentially with alumina slurries of 0.3 and 0.05 μm to create a mirror finish; then, the electrodes were washed sequentially in acetone, ethanol and pure water with sonication each for 10 min and dried with nitrogen gas. In all of the procedures, we used pure water prepared with a Kertone Ultrapure Water System P60-CY (Kertone Water Treatment Co., Ltd; resistivity, > 18 MΩ•cm).
Solutions of glucose, AA, UA and AP were prepared using PBS before the electrochemical test. Glassy carbon plates (size 10 mm × 10 mm × 1.0 mm) and gold bulk electrodes (diameter, 2.0 mm) were ordered from BAS Co., Ltd. The GC plates were polished sequentially with alumina slurries of 0.3 and 0.05 μm to create a mirror finish; then, the electrodes were washed sequentially in acetone, ethanol and pure water with sonication each for 10 min and dried with nitrogen gas. In all of the procedures, we used pure water prepared with a Kertone Ultrapure Water System P60-CY (Kertone Water Treatment Co., Ltd; resistivity, > 18 MΩ•cm).
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