Hp 1100 hplc

Manufactured by Agilent Technologies

Sourced in United States

The HP 1100 HPLC is a high-performance liquid chromatography system designed for analytical and preparative applications. It features a quaternary pump, an autosampler, a diode-array detector, and a thermostatted column compartment. The system is capable of performing a variety of chromatographic separations and is suitable for a range of sample types and analyses.

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Lab products found in correlation

C di amp, by Biolog (1 mentions) C di gmp, by Biolog (1 mentions) Chiralcel oj h column, by Daicel (1 mentions) Hp 9000, by Agilent Technologies (1 mentions) Quercetin, by Extrasynthese (1 mentions) Kaempferol, by Extrasynthese (1 mentions) Mini protease inhibitor cocktail tablet, by Roche (1 mentions) Galangin, by Extrasynthese (1 mentions) Gemini c18, by Phenomenex (1 mentions) Uridine 5 diphosphoglucuronic acid, by Merck Group (1 mentions) Millicell ers voltohmmeter, by Merck Group (1 mentions)

Close spelling variants of this product

HP 1100 Series HPLC Agilent HP-1100 HPLC system 1100 HPLC

8 protocols using hp 1100 hplc

Characterization of MNPs-Apt Conjugates

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The
MNPs-SA with the size of 300 nm was provided by BioMag Scientific
(2 mg/mL. Wuxi, China). The FL signal was measured by an FS5 FL spectrophotometer
(Edinburgh Instruments, UK). A Nanodrop 2000 ultrafine ultraviolet
spectrophotometer (Thermo Fisher Scientific) was used to identify
the nucleic acid aptamers’ reaction. A Zeta sizer Nano-ZS 90
(laser particle size and zeta potential analyzer, Malvern, UK) was
applied to explore the hydrodynamic diameter and zeta potential of
MNPs-Apt conjugates, which were prepared from bio-Apt with MNPs-SA
by particular affinity. In addition, A JEM2100 transmission electron
microscope (Japan) was used to depict the morphology characterization
of the conjugates. An electrophoresis apparatus was applied to perform
the electrophoresis experiment to ensure the smooth progress of the
strand displacement amplification reaction. Another instrument used
in this paper for comparative analysis was HP 1100 HPLC (Agilent Technologies,
USA).

Wu R., Guo J., Wang M., Liu H., Ding L., Yang R., Liu L.E, & Liu Z. (2023). Fluorescent Sensor Based on Magnetic Separation and Strand Displacement Amplification for the Sensitive Detection of Ochratoxin A. ACS Omega, 8(17), 15741-15750.

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HPLC-UV Quantification of Intracellular Nucleotides

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Separation and quantification of intracellular nucleotides by HPLC-UV were performed essentially by the method of Strauch et al. (48 (link)) using an Agilent HP1100 HPLC. Briefly, aliquots of nucleotide extracts were separated at 20°C by anion-exchange chromatography using a Partisil 10SAX column (10-μm particle size; 25 cm by 4.6 mm internal diameter). Gradient elution was performed by the following gradient elution program using mobile phase A (MPA) (7 mM potassium hydrogen phosphate [pH 4.0]) and mobile phase B (MPB) (0.5 M potassium dihydrogen phosphate–0.5 M sodium sulfate pH 5.4) and a flow rate of 1.5 ml/min: 0 to 5 min, 100% MPA; 5 to 10 min, 100% to 85% MPA; 10 to 15 min, 85% to 81% MPA; 15 to 20 min, 81% to 50% MPA; 20 to 25 min, 50% to 30% MPA; 25 to 30 min, 30% to 25% MPA; 30 to 40 min, 30% to 0% MPA (postrun equilibration, 100% MPA for 15 min). Eluting nucleotides were detected using a UV diode-array detector and quantified by their absorbance at 254 nm in comparison to known standards. Quantified values in picomoles were normalized to the cells present in 1 ml of a culture with an OD₆₀₀ value of 1. All nucleotide standards were purchased from Sigma, with the exception of cdiAMP, cdiGMP, pApA, and pGpG which came from Biolog.

Hesketh A., Vergnano M, & Oliver S.G. (2019). Determination of the Global Pattern of Gene Expression in Yeast Cells by Intracellular Levels of Guanine Nucleotides. mBio, 10(1), e02500-18.

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HPLC Analysis of Organic Compounds

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HPLC analysis was carried out on an Agilent HP 1100 HPLC. Elution was performed using an isocratic mobile phase of acetonitrile and water (55:45, v/v) at a flow rate of 1.2 ml/min. The column temperature was 25 °C, and the injection volume was 20 µl. A reversed-phase C₁₈ column (4.6 mm × 150 mm, 5 µm, Bischoff Chromatography, Leonberg, Germany) was used, and the chromatograms were monitored by an ultraviolet detector set at 240 nm.

Pham C.V., Baek J.S., Park J.H., Jung S.H., Kang J.S, & Cho C.W. (2018). A thorough analysis of the effect of surfactant/s on the solubility and pharmacokinetics of (S)-zaltoprofen. Asian Journal of Pharmaceutical Sciences, 14(4), 435-444.

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Chiral HPLC Analysis of ZPF Stereoisomers

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Chiral HPLC analysis was carried out on an Agilent HP 1100 HPLC using a Chiralcel OJ-H column (4.6 mm × 150 mm, 5 µm, Daicel Corp., Tokyo, Japan). The mobile phase, hexane-isopropanol-triflouroacetic acid (90:10:0.1, v/v/v), was filtered (0.45 µm), degassed, and delivered at a flow rate of 0.8 ml/min. The column temperature was maintained at 25 °C, and the injection volume was 20 µl. (RS)-ZPF, (R)-ZPF, and (S)-ZPF were detected at 240 nm.

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HPLC Analysis of Saponin Compounds

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The analysis of saponin was run on HP 1100 HPLC (Agilent Company, USA) in the following operating conditions: column: Hypersil ODS (250 × 4.6 mm, 5 μm); flow phase: methanol/water (80/20); injection volume: 10 μl; flow rate: 1 ml/min; temperature: 25 °C; wavelength: 209 nm [31 ].

Ye Y., Yang Q., Fang F, & Li Y. (2015). The camelliagenin from defatted seeds of Camellia oleifera as antibiotic substitute to treat chicken against infection of Escherichia coli and Staphylococcus aureus. BMC Veterinary Research, 11, 214.

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HPLC Analysis of Polyphenol Extracts

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POE analyses were performed with an HP 1100 HPLC (Agilent Technologies, Santa Clara, CA, USA) equipped with DAD detector and a Luna Omega Polar (150 × 3 mm, 5 µm) (all from Agilent Technologies) RP-C18 analytical column. The software was HP 9000 (Agilent Technologies). The mobile phase consisted of (A) formic acid/water pH 3.2 and (B) acetonitrile. The following gradient profile was applied: 0–2 min 95% A and 5% B; 2–8 min 75% A and 25% B; 8–10 min 70% A and 30% B; 10–12 min 70% A and 30% B; 12–16 min 60% A and 40% B; 16–18 min 40% A and 60% B; 18–22 min 5% A and 95% B; 22–23 min 5% A and 95% B; 23–25 min 95% A and 5% B. The flow rate was 0.5 mL/min, injection volume 10 μL, and the temperature 25 °C. The calibration curve was prepared using a standard solution of catechin (0.435 mg/mL) diluted 10, 20, 50, 100, 200, and 500 times. The concentration absorption relationship was above than 0.9996. The chromatographic profile of POE polyphenols was acquired at 260 nm and the total polyphenol content was expressed as catechin, using the external standard method.

Piazzini V., Vasarri M., Degl’Innocenti D., Guastini A., Barletta E., Salvatici M.C, & Bergonzi M.C. (2019). Comparison of Chitosan Nanoparticles and Soluplus Micelles to Optimize the Bioactivity of Posidonia oceanica Extract on Human Neuroblastoma Cell Migration. Pharmaceutics, 11(12), 655.

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Caco-2/TC7 Cell Culture Protocol

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All cell culture supplies were from Invitrogen, Paisley, UK, unless otherwise stated. Caco-2/TC7 cells were kindly donated by Dr M. Rousset, U178 INSERM, Villejuif, France. Millicell-ERS volt ohmmeter was obtained from Millipore corporation, Massachusetts, USA. Transwell inserts and 12 well plates were Costar brand obtained from Fisher Scientific, Loughborough, UK. The MK571 was purchased from Biomol Research Laboratories, Exeter, UK. Mini protease inhibitor cocktail tablets containing EDTA and perfabloc were purchased from Roche, Welwyn Garden City, UK. Galangin, quercetin and kaempferol were purchased from Extrasynthese, 69727 Genay Cedex, France. Alamethacin from Trichoderma viride, uridine 5′-diphosphoglucuronic acid and uridine 5′-diphospho-N-acetyl-glucosamine were purchased from Sigma–Aldrich, Poole, UK. GraFit software was version 4.0 and purchased from Erithacus Software Ltd., East Grinstead, UK. Gemini C18 (150 mm × 2.0 mm i.d., 5 μm) narrow bore HPLC column was purchased from Phenomenex, Macclesfield, UK. Analytical chemistry was performed using ChemStation software on an HP1100 HPLC and single quadrupole MSD SL mass spectrometer from Agilent, Manchester, UK. All solvents and other reagents of analytical grade were obtained from Sigma–Aldrich, Poole, UK.

Barrington R.D., Needs P.W., Williamson G, & Kroon P.A. (2015). MK571 inhibits phase-2 conjugation of flavonols by Caco-2/TC7 cells, but does not specifically inhibit their apical efflux. Biochemical Pharmacology, 95(3), 193-200.

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Quantification of Rosmarinic Acid in Lemon Balm Extract

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This study used commercially available lemon balm (Melissa officinalis L., Lamiaceae) leaf extract, standardized at a minimum of 7% RA. This extract is formulated as capsules and registered as a dietary supplement. In order to validate the content of RA, a previously described analytical method was applied [47 ]. In brief, high-performance liquid chromatography (HPLC-DAD) was performed (Agilent HP 1100 HPLC coupled with diode array detector - DAD); separation of the components was achieved on a Nucleosil C18 (5 μm × 4.6 mm × 250 mm) column heated to 30 °C. A gradient elution was applied, with 1% formic acid being mobile phase A and methanol being mobile phase B; the program of elution was as follows: 0–10 min, 10–25% B; 10–20 min, 25–45% B; 20–30 min, 45% B; 30–35 min, 45–70% B; 35–40 min, 70–100% B; 40–43 min, 100% B. The chromatogram of RA was monitored at 330 nm. The result is expressed as a percentage of the declared content.

Hitl M., Pavlović N., Brkić S., Dragović G., Srđenović-Čonić B, & Kladar N. (2024). Plasma Concentrations of Rosmarinic Acid in Patients on Antiretroviral Therapy: In Silico Exploration Based on Clinical Data. International Journal of Molecular Sciences, 25(4), 2230.

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