Electron microscope film 4489

Samples used for histological analysis were further processed for electron microscopy. Resin blocks were cut in 90-nm sections on an ultramicrotome (Reichert Ultracut E) with a diamond knife (Diatome) and visualized using a transmission electron microscope (Hitachi H600). Random images (saline n=10 and TTX n=12) from the lesions were taken and developed on Electron Microscope Film 4489 (Kodak), then scanned at high resolution (12,800 dpi × 12,800 dpi). The number of axons remyelinated and their g-ratios (the ratio of axon diameter to the outside diameter of the myelin) were analysed with ImageJ software (version 1.45 s) by blinded analysis. A total of 302 (TTX) and 312 (saline) axons were analysed.

An inclusion body fraction of semi-purified Nipah N antigen was prepared by negative contrast electron microscopy. Specifically, 20 μl of sample was absorbed to formvar-coated carbon-stabilized copper grids and stained with 2% phosphotungstic acid, pH 7.2 (wt/vol). The specimen grids were examined in a Philips CM 120 transmission electron microscope, operating at an accelerating voltage of 80 kV. Micrographs were taken between 28,000X -45,000X using Kodak Electron Microscope Film 4489. The negatives were scanned using an Epson Perfection 3200 photo scanner and enlarged 2.5X.