A Maxis II type Qq-TOF MS instrument (Bruker Daltoniks, Bremen, Germany) equipped with an Apollo II electrospray ion source was used. The spray voltage was 4.5 kV. The resolution of the instrument was 40,000 at m/z 400 (FWHM), and the mass accuracy was <2 ppm (external calibration). N2 was utilized as the drying gas (200 °C, 4.0 L/min), nebulizer gas (0.5 bar) and collision gas. The collision voltage was varied in the range of 20–90 V. The mass spectra were recorded by means of a digitizer at a sampling rate of 2 GHz. The spectra were calibrated externally by ESI tune mix, from Bruker. The spectra were evaluated with the Compass Data Analysis 4.4 software from Bruker (Bremen, Germany). The sample solutions were introduced directly into the ESI source with a syringe pump (Cole-Parmer Ins. Co., Vernon Hills, IL, USA) at a flow rate of 3 μL/min. The concentration of the samples was 0.01 mg/mL.
Esi tune mix
Manufactured by Bruker
Sourced in Germany
The ESI Tune Mix is a liquid calibration solution designed for use with electrospray ionization (ESI) mass spectrometry instruments. It provides a range of known compounds and their corresponding mass-to-charge (m/z) values, allowing for accurate mass calibration and tuning of the instrument.
Automatically generated - may contain errors
Lab products found in correlation
Compass dataanalysis 4, by Bruker (2 mentions)
Maxis 2 type qq tof ms instrument, by Bruker (2 mentions)
Elute uhplc, by Bruker (1 mentions)
T1500, by Merck Group (1 mentions)
Compact qtof, by Bruker (1 mentions)
Reprosil gold 120 c18, by Dr. Maisch (1 mentions)
Impact 2 q tof spectrometer, by Bruker (1 mentions)
4 protocols using esi tune mix
1
High-Resolution Qq-TOF Mass Spectrometry Protocol
2
Testosterone Analysis by LC-MS/MS
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MS/MS analysis was performed on an Elute UHPLC interfaced with a Compact Q-TOF (Bruker Daltonics). The nonpolar extract was prepared in MeOH + 0.1% FA (Formic acid) at 10 μg/mL, and a commercial standard of testosterone (T1500, Sigma-Aldrich, St. Louis, MO, USA) was prepared in MeOH + 0.1% FA to 10 mM. Samples (5 µL) were analyzed on a C18 column (C18 Poroshell UPLC 1.9 µm (2.1 × 1.5 mm)) using an isocratic gradient at 60% B (A: H20 + 0.1% FA, B: MeOH + 0.1% FA) over 10 min. The instrument was internally calibrated using ESI Tune Mix (Bruker Daltonics) and set to the following parameters: Mass range: m/z 50–2000, Positive mode, Auto MS/MS with 9 precursor ions per cycle. Testosterone was fragmented at 35 eV.
3
Mass Spectrometric Characterization of Oxidized Peptides
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The molecular weight of the purified and oxidized peptides was confirmed by ESI mass spectrometry on a micrOTOF-Q III or TOF-Q impact II spectrometer (Bruker Daltonik GmbH, Bremen, Germany) and calibrated using Bruker’s ESI-Tune-Mix. LC/MS analysis was performed on a TOF-Q impact II spectrometer (Bruker Daltonik GmbH, Bremen, Germany) equipped with a Agilent 1200 HPLC system using a C18 column (Reprosil Gold 120, C18, 3.0 µm particle size, 120 Å pore size, 100 × 2 mm, Dr. Maisch GmbH, Ammerbuch-Entringen, Germany). The oxidized peptides were eluted with a gradient of 5–95% eluent B in 20 min at a flow rate of 0.2 mL/min. Chromatograms were extracted at 210–220 nm.
4
Qq-TOF MS Analysis of Samples
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A Maxis II type Qq-TOF MS instrument (Bruker Daltonik, Bremen, Germany) equipped with an Apollo II electrospray ion-source was used in positive ion mode. The spray voltage was 4.0 kV. The resolution of the instrument was 40 000 at m/z 400 (FWHM), and the mass accuracy was better than 1 ppm (external calibration). N2 was utilized as drying gas (200 °C, 4.0 L/min), nebulizer gas (0.5 bar) and collision gas. The collision energy was varied in the range of 50–130 eV in the laboratory frame. All precursor ions were selected with an isolation width of m/z 2.00. The mass spectra were recorded with a digitizer at a sampling rate of 2 GHz. The spectra were calibrated externally by ESI tune mix provided by Bruker Daltonik (Bremen, Germany). The spectra were evaluated with Compass Data Analysis 4.4 software (Bruker, Bremen, Germany). The sample solutions were introduced directly into the ESI source with a syringe pump (Cole-Parmer Ins. Co., Vernon Hills, IL, USA) at a flow rate of 3 μL/min.
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