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4 protocols using fmoc lys boc oh

1

Multifunctional Nanoparticle Formulation

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Gambogic acid (GA) and etoposide (VP16) were purchased from AvaChem Scientific. Oridonin (ORD) was obtained from Xi’an Xuhuang Bio-tech Co., Ltd. Triptolide (TPL) was purchased from A Chemtek Inc. Paclitaxel (PTX) was purchased from AK Scientific Inc. Ltd. Monomethoxyl terminated polyethylene glycol monoamine (MeO-PEG-NH2, MW: 5000 Da) was purchased from JenKem. (Fmoc)lys(Boc)-OH, (Boc)Lys(Boc)-OH, (Fmoc)Lys(Fmoc)-OH and (Fmoc)Ebes-COOH were purchased from AnaSpec Inc. A hydrophobic cyanine dye of DiI, and a hydrophobic near infrared fluorescence dye of 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindodicarbocyanine perchlorate (DiD) were purchased from AAT Bioquest, Inc. CellTiter 96® AQueous MTS reagent powder was purchased from Promega. Cholic acid (CA), D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS, PEG1kVE), D-α-tocopherol succinate, diisopropyl carbodimide (DIC), N-hydroxybenzotriazole (HOBt), and all other chemical reagents were purchased from Sigma-Aldrich. Dialysis membrane with 3500 MW cut off was purchased from Spectrum Laboratories, Inc.
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2

Fluorescence-based Cell Adhesion Assay

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All chemicals and reagents were
of analytical grade and used without further purification. Common
chemicals were obtained from Fischer Scientific (Pittsburgh, PA) or
Sigma-Aldrich (St. Louis, MO) unless specified. Rink amide 4-methylbenzylhydrylamine
(MBHA) resin, amino acids [Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Gly-OH,
Fmoc-Ser(tBu)-OH, Fmoc-Thr(tBu)-OH, Fmoc-Lys(Boc)-OH, and Fmoc-Lys(N3)-OH], Boc-aminooxyacetic acid, Fmoc-ε-Ahx-OH, and HBTU
were purchased from Anaspec (San Jose, CA). Antibodies antipaxillin
and antivinculin and Cy-2 goat anti-mouse IgG were purchased from
BD Biosciences (San Jose, CA) and Jackson ImmunoResearch Laboratories,
Inc. (West Grove, PA), respectively. Fluorescent dyes DAPI and phalloidin
and penicillin/streptomycin were obtained from Invitrogen (Carlsbad,
CA). Fluorescence mounting medium was purchased from Dako (Carpinteria,
CA). Swiss albino 3T3 mouse fibroblasts were obtained from the UNC-CH
Tissue Culture Facility (Chapel Hill, NC). Heparan sulfate, heparinase
I and II, and chondroitinase ABC were obtained from Sigma-Aldrich
(St. Louis, MO).
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3

Synthesis of Multifunctional PEGylated Conjugates

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Monomethylterminated poly(ethylene glycol) monoamine hydrochloride (MeO-PEG-NH2·HCl, MW: 5 kDa) was purchased from Jenkem Technology. (Fmoc)Lys(Fmoc)-OH and (Fmoc)Lys(Boc)-OH were obtained from AnaSpec Inc. Cholesteryl chlorofomate was purchased from Alfa Aesar. CellTiter 96® AQueous MTS reagent powder was purchased from Promega. Diisopropyl carbodimide (DIC), N-hydroxybenzotriazole (HOBt), trifluoroacetic acid (TFA), methyl chlorooxoacetate, fluorescein isothiocyanate isomer I (FITC), bovine serum albumin (BSA, Mw 66.5 kDa), N-hydroxysuccinimide (HOSu), rhodamine B (RB), polyethylenimine (PEI, branched, Mw 25 kDa) and other chemical reagents were purchased from Sigma-Aldrich. Dialysis membrane with 3,500 MW cut off was purchased from Spectrum Laboratories, Inc.
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4

Synthesis and Characterization of Doxorubicin-Conjugated Theranostic Dendrimers

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Doxorubicin hydrochloride (DOX·HCl) was purchased from AvaChem Scientific LLC (San Antonio, TX), and Doxil® (Ben Venue Laboratories Inc., Bedford, OH) was obtained from the Regional Oncology Center Pharmacy, State University of New York (SUNY) Upstate Medical University. Monomethyl-terminated poly(ethylene glycol) monoamine hydrochloride (MeO-PEG-NH2·HCl, Mw 5 kDa) was purchased from JenKem Technology USA Inc. (Fmoc)Lys(Boc)-OH, and (Fmoc)Lys(Fmoc)-OH were purchased from AnaSpec Inc. (San Jose, CA). CellTiter 96® AQueous MTS reagent powder was purchased from Promega (Madison, WI, USA). Rf, CA, glutaric anhydride and all other chemicals were purchased from Sigma-Aldrich (St. Louis). The preparation of CA derivatives (CA NHS ester) was described in the previous publication.[42 (link)]
The nomenclatures of the TDs follow the system used in our previous publications.[42 (link), 43 , 58 (link)] For example, TD named PEG5kRf8 means that the molecular weight of PEG is 5 kDa with eight Rf molecules conjugated on the periphery amino groups of polylysine; PEG5kCA4Rf4 indicates that four CA and four Rf molecules are conjugated on the α-amino and ε-amino groups, respectively.
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