On day 1, mice were habituated to the shock chamber (Coulbourn Instruments) for 5 min. On day 2, a mouse spent 3 min in the chamber before it received foot shocks (0.8 mA) at 3-, 4-, and 5-min time points, and freezing levels during 3–6 min were quantified. On day 3, mice were re-exposed to the chamber without foot shock for 5 min, and freezing levels during this 5 min were quantified. Freezing behavior was analyzed using FreezeFrame 3 software (Coulbourn Instruments). For contextual fear extinction, mice were exposed to the same shock chamber for 5 min for 10 consecutive days.
Shock chamber
Manufactured by Harvard Apparatus
The Shock Chamber is a piece of lab equipment designed to deliver controlled electrical shocks to experimental subjects. It is used to study the physiological responses of organisms to electrical stimuli. The device allows researchers to precisely control the voltage, duration, and frequency of the electrical pulses delivered to the subject.
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Lab products found in correlation
2 protocols using shock chamber
1
Fear Conditioning and Extinction in Mice
2
Investigating the Effects of IGFBP2 and IGF1 on Contextual Fear Extinction
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Testing was conducted as previously described (Burgdorf et al., 2015b (link)), and the first extinction tests occurred 1 hour postdosing. On the contextual fear training day (D0), animals were placed in a Coulbourn Instruments shock chamber (40 × 40 × 40 cm) for 400 seconds and received three 0.5-mA 1-s footshocks delivered to the floor bars at 90-, 210-, and 330-second timepoints. During extinction, rats were subjected to daily 5-minute nonreinforced (no shock) extinction trials for the first 6 days after training and on day 14 posttraining (consolidation trial). Freezing was quantified via FreezeFrame software (Actimetrics) during the last 3 minutes of each extinction trial. Animals were dosed with a single optimal dose of IGFBP2 (1 µg/kg i.v.), IGF1 (100 µg/kg i.v.), or sterile saline vehicle (1 mL/kg i.v.) 1 hour before the first extinction session (n = 9–11/group).
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