E faecalis

Manufactured by China General Microbiological Culture Collection Center

Sourced in China

E. faecalis is a strain of bacteria maintained in the China General Microbiological Culture Collection Center. It serves as a standard reference material for microbiological research and testing applications.

Automatically generated - may contain errors

Lab products found in correlation

6 protocols using e faecalis

Microbial Culture and Plasmid Acquisition

Check if the same lab product or an alternative is used in the 5 most similar protocols

EcN, E. faecalis, S. aureus, and Salmonella typhimurium were purchased from China general microbiological culture collection center (GMCC, China), cell lines (CT26 and 4T1) were obtained from American Type Culture Collections (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (Sigma, USA) supplemented with 10% (v/v) inactivated FBS (Sigma, USA) and 1% (v/v) antibiotic/anti-mycotic solution (Sigma, USA) at 37 °C incubator with 5% CO₂. Regular mycoplasma evaluations were performed of the cell culture environment to ensure the absence of mycoplasma contamination. Plasmids pBBR1MCS2-Tac-mCherry, pBBR1MCS2-Tac-GFP, pMD18-luxCDABE and all other reagents were purchased from domestic suppliers and used as received.

Cao Z., Cheng S., Wang X., Pang Y, & Liu J. (2019). Camouflaging bacteria by wrapping with cell membranes. Nature Communications, 10, 3452.

+ Open protocol

+ Expand

Antimicrobial Potency Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The strains were purchased from China General Microbiological Culture Collection Center (CGMCC), Beijing, China, including S. aureus (CGMCC 1.2465), L. monocytogenes (CGMCC 1.10753), E. faecalis (CGMCC 1.2135), Enterococcus faecium (CGMCC 1.1310), Staphylococcus epidermidis (CGMCC 1.4260), P. aeruginosa (CGMCC 1.2421), E. coli (CGMCC 1.2389), A. baumannii (CGMCC 1.6769), C. neoformans (CGMCC 2.1563), F. graminearum (CGMCC 3.4521), F. solani (CGMCC 3.5840), and A. niger (CGMCC 3.3160). The bacterial and fungal strains were cultured in nutrient broth (OXOID, UK) agar at 37 °C and yeast extract peptone dextrose (OXOID, UK) agar at 28 °C.
Cells (including murine macrophage RAW 264.7, human embryonic kidney 293 cell (HEK-293T), and hepatocellular carcinoma cell line HepG2) were obtained from the Stem Cell Bank at the Chinese Academy of Sciences (Shanghai, China).
LL-37 was synthesized from GL Biochem (Shanghai, China), and the antibiotic polymyxin B (PMB) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

Jiang M., Chen R., Zhang J., Chen F, & Wang K.J. (2022). A Novel Antimicrobial Peptide Spampcin56–86 from Scylla paramamosain Exerting Rapid Bactericidal and Anti-Biofilm Activity In Vitro and Anti-Infection In Vivo. International Journal of Molecular Sciences, 23(21), 13316.

+ Open protocol

+ Expand

Probiotic Strains Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Enterococcus faecalis (E. faecalis, CGMCC1.2135) and Saccharomyces cerevisiae (S. cerevisiae, CGMCC 2.1542) used in the experiment were purchased from China General Microbiological Culture Collection Center (CGMCC), Beijing, China. E. faecalis and S. cerevisiae were incubated in MRS and YPD liquid media according to the previous report, respectively (Liu et al. 2019 (link)). The fermentation liquid of above probiotics were harvested after 36 h culture and determined by plating serial dilutions and measured as colony forming units (CFU), and then centrifuged at 8000 r/min for 5 min, the supernatant was absorbed, sterilized by 0.22 μm Minisart high-flow filter and stored at 4 °C for further use. The centrifuged cells were resuspended in equal volume using High-glucose DMEM medium without serum and antibiotics. The fermentation liquid, supernatant and cells were diluted to the different concentrations (viable counts of 1 × 10², 1 × 10³, 1 × 10⁴, 1 × 10⁵ and 1 × 10⁶ CFU/mL) with High-glucose DMEM without serum and antibiotics.

Xu X., Chang J., Wang P., Liu C., Zhou T., Yin Q, & Yan G. (2023). Glycyrrhinic acid and probiotics alleviate deoxynivalenol-induced cytotoxicity in intestinal epithelial cells. AMB Express, 13, 52.

+ Open protocol

+ Expand

Optimizing Probiotic-Glycyrrhizic Acid Formulation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glycyrrhizic acid was provided by Henan Delin Biological Products Co. Ltd., Xinxiang, China. Saccharomyces cerevisiae (S. cerevisiae, CGMCC 2.1542) and Enterococcus faecalis (E. faecalis, CGMCC1.2135) used in this experiment were purchased from China General Microbiological Culture Collection Center (CGMCC), Beijing, China. They were incubated according to the published protocols [40 (link)] and then freeze dried. Based on our preliminary experimental results obtained with orthogonal design in vitro, the optimal ratio of GA and compound probiotics (GAP) were 400 mg/kg GA, 1 × 10⁶ CFU/g S. cerevisiae and 1 × 10⁶ CFU/g E. faecalis.

Xu X., Chang J., Wang P., Liu C., Liu M., Zhou T., Yin Q, & Yan G. (2022). Glycyrrhizic Acid and Compound Probiotics Supplementation Alters the Intestinal Transcriptome and Microbiome of Weaned Piglets Exposed to Deoxynivalenol. Toxins, 14(12), 856.

+ Open protocol

+ Expand

Bacterial Culture and Preparation for Experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols

B. subtilis (CGMCC 1.3358), E. faecium (CGMCC 1.2136), and E. faecalis (CGMCC 1.2135) were obtained from China General Microbiological Culture Collection Center (Beijing, China). These bacteria were respectively inoculated into the corresponding liquid medium and cultured at 37 °C for 24 h (B. subtilis: nutrient broth medium, HB0105, Hope Biotechnology Co., Ltd., Qingdao, China; E. faecium: TSB, HB4114, Hope Biotechnology Co., Ltd., Qingdao, China; E. faecalis: TSB, HB4114, Hope Biotechnology Co., Ltd., Qingdao, China). Then they were diluted in the corresponding media, and continued to be cultured to reach the logarithmic stage with an optical density (OD) of 0.5 detected at a wavelength of 600 nm. The supernatants were collected by centrifugation (5000 g, 10 min) and filtered twice through a 0.22 μm filter.
Campylobacter jejuni 81-176 (BAA-2151; ATCC, Manassas, VA, United States) was grown on Skirrow’s selective medium (Columbia Agar Base, Oxoid CM0331) at 42 °C for 24 h. Bacterial colonies were obtained with an inoculating loop and diluted with culture medium until reaching a concentration of 10¹⁰ CFU/mL. Determination of bacterial concentration was carried out by the conventional plate-counting method.

Chen Y.M., Li Y., Wang X., Wang Z.L., Hou J.J., Su S., Zhong W.L., Xu X., Zhang J., Wang B.M, & Wang Y.M. (2022). Effect of Bacillus subtilis, Enterococcus faecium, and Enterococcus faecalis supernatants on serotonin transporter expression in cells and tissues. World Journal of Gastroenterology, 28(5), 532-546.

+ Open protocol

+ Expand

Microbial Strain Acquisition and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

All commercially available strains used in this study were purchased from China General Microbiological Culture Collection Center (CGMCC), including L. monocytogenes (CGMCC NO. 1.10753), E. faecium (CGMCC NO. 1.131), E. faecalis (CGMCC NO. 1.2135), S. aureus (CGMCC NO. 1.879), S. epidermidis (CGMCC NO. 1.4260), B. subtilis (CGMCC NO. 1.3358), A. baumanii (CGMCC NO. 1.6769), P. aeruginosa (CGMCC NO. 1.2421), P. stutzeri (CGMCC NO. 1.1803), P. fluorescens (CGMCC NO. 1.3202), E. coli (CGMCC NO. 1.2389), S. fiexneri (CGMCC NO. 1.1868), F. oxysporum (CGMCC NO. 3.6785), F. solani (CGMCC NO. 3.5840), F. graminearum (CGMCC NO. 3.3490), A. niger (CGMCC NO. 3.316), A. fumigatus (CGMCC NO. 3.5835) and A. ochraceus (CGMCC NO. 3.5830). Bacterial strains grew at 37 °C in nutrient broth (OXOID, Hampshire, UK) and fungal strains were cultured in potato dextrose agar (Hope Bio, Qingdao, China) at 28 °C, respectively.

Chen Y.C., Qiu W., Zhang W., Zhang J., Chen R., Chen F, & Wang K.J. (2022). A Novel Antimicrobial Peptide Sp-LECin with Broad-Spectrum Antimicrobial Activity and Anti-Pseudomonas aeruginosa Infection in Zebrafish. International Journal of Molecular Sciences, 24(1), 267.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!