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Mouse antibody against flag tag

Manufactured by Merck Group

The mouse antibody against Flag tag is a laboratory reagent used to detect and purify proteins that have been engineered to contain a Flag tag sequence. The Flag tag is a short peptide sequence that can be added to proteins to facilitate their identification and isolation. The mouse antibody binds specifically to the Flag tag, allowing the tagged protein to be detected or isolated from a complex mixture.

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2 protocols using mouse antibody against flag tag

1

Antibody Reagents for STING Signaling

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The rabbit polyclonal antibodies against human STING were described previously23 (link). Rabbit antibodies against mouse STING, p-IRF3(Ser396), p-TBK1(Ser172), p-IKKβ(Ser177), ATG5, ATG9, Beclin1, calreticulin, and GAPDH were from Cell Signaling. Mouse antibody against STING was purchased from R&D Systems; rabbit antibodies against human IRF3, TGN38, and ARF1 and mouse antibody against CD63 were from Santa Cruz Biotechnology; rabbit antibody against LC3 was from Novus Biologicals; mouse antibodies against P62 and GGA3 were from BD Transduction Laboratories; mouse antibody against ERGIC-53 was from Axxora; rabbit antibody against Sec22b was from Synaptic Systems; mouse antibody against Flag tag, rabbit antibodies against ERGIC53, and β-tubulin, anti-Flag (M2)-conjugated agarose and anti-HA–conjugated agarose were from Sigma; HA antibody were from Covance; rabbit antibodies against GBF1, LAMP2, and Giantin were from Abcam; rabbit antibody against beta-COP was from Thermo Fisher; rabbit antibodies against ARFGEF1, ARFGEF2, and SEC24C were from Bethyl Laboratories.
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2

Antibody Reagents for STING Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The rabbit polyclonal antibodies against human STING were described previously23 (link). Rabbit antibodies against mouse STING, p-IRF3(Ser396), p-TBK1(Ser172), p-IKKβ(Ser177), ATG5, ATG9, Beclin1, calreticulin, and GAPDH were from Cell Signaling. Mouse antibody against STING was purchased from R&D Systems; rabbit antibodies against human IRF3, TGN38, and ARF1 and mouse antibody against CD63 were from Santa Cruz Biotechnology; rabbit antibody against LC3 was from Novus Biologicals; mouse antibodies against P62 and GGA3 were from BD Transduction Laboratories; mouse antibody against ERGIC-53 was from Axxora; rabbit antibody against Sec22b was from Synaptic Systems; mouse antibody against Flag tag, rabbit antibodies against ERGIC53, and β-tubulin, anti-Flag (M2)-conjugated agarose and anti-HA–conjugated agarose were from Sigma; HA antibody were from Covance; rabbit antibodies against GBF1, LAMP2, and Giantin were from Abcam; rabbit antibody against beta-COP was from Thermo Fisher; rabbit antibodies against ARFGEF1, ARFGEF2, and SEC24C were from Bethyl Laboratories.
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