All reagents were used as received unless otherwise stated. GEO5MA monomer was synthesized by Dr C. P. Jesson at GEO Specialty Chemicals (Hythe, UK) as previously described. 59 2-Hydroxypropyl methacrylate (HPMA, 97%) was kindly donated by GEO Specialty Chemicals (Hythe, UK). 4,4'-Azobis(4cyanopentanoic acid) (ACVA; >98%), glycine (≥98%), histidine (≥98%), ethylene glycol dimethacrylate (EGDMA, 98%), sodium periodate (NaIO 4 , ≥99.8%), sodium cyanoborohydride (NaCNBH 3 , 95%), bovine serum albumin (BSA) and deuterium oxide (D 2 O) were purchased from Sigma-Aldrich, UK. 2-Cyano-2propyl dithiobenzoate (CPDB, >97%) was purchased from Strem Chemicals Ltd (Cambridge, UK).
Ethanol, dichloromethane and diethyl ether were purchased from Fisher Scientific (UK). Aqueous electrophoresis. Zeta potentials for diblock copolymer nanoparticles were analyzed using a Malvern Zetasizer Nano ZS instrument equipped with a 4 mW He-Ne laser (λ = 633 nm) operating at a fixed scattering angle of 173°. Samples were diluted to 0.1% w/w using 1 mM KCl, with either dilute NaOH or HCl being used for pH adjustment as required. Zeta potentials were calculated from the Henry equation using the Smoluchowski approximation.
Centrifugation. Centrifugation of BSA functionalized vesicles was conducted using a Thermo Heraeus Biofuge Pico centrifuge.
Ethanol, dichloromethane and diethyl ether were purchased from Fisher Scientific (UK). Aqueous electrophoresis. Zeta potentials for diblock copolymer nanoparticles were analyzed using a Malvern Zetasizer Nano ZS instrument equipped with a 4 mW He-Ne laser (λ = 633 nm) operating at a fixed scattering angle of 173°. Samples were diluted to 0.1% w/w using 1 mM KCl, with either dilute NaOH or HCl being used for pH adjustment as required. Zeta potentials were calculated from the Henry equation using the Smoluchowski approximation.
Centrifugation. Centrifugation of BSA functionalized vesicles was conducted using a Thermo Heraeus Biofuge Pico centrifuge.