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Cholesterol cho

Manufactured by Merck Group
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Cholesterol (CHO) is a laboratory equipment product designed to measure and analyze cholesterol levels. It is a core component in various biochemical and clinical testing procedures. The device provides accurate and reliable cholesterol measurements, which are essential for assessing an individual's cardiovascular health and risk factors.

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Lab products found in correlation

3 3 dioctadecyloxacarbo cyanine perchlorate, by Thermo Fisher Scientific (1 mentions) 1 1 dioctadecyl 3 3 3 3 tetramethylindotricarbocyanineiodide, by Thermo Fisher Scientific (1 mentions) Dspe mpeg2000, by Avanti Polar Lipids (1 mentions) Mpeg2000, by Avanti Polar Lipids (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Cell culture media, by Corning (1 mentions) Disodium hydrogen phosphate na2hpo4, by Merck Group (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Milli q millipore, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions) Glucose, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Vitamin d3 cholecalciferol, by Merck Group (1 mentions) 3 palmitoyl sn glycerol, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Glacial acetic acid, by Merck Group (1 mentions) Chitosan, by Merck Group (1 mentions)

9 protocols using cholesterol cho

1

Lipid-based Drug Delivery Formulation

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Lipids,
including egg phosphatidylcholine
(EPC) and distearylphosphatidylethanolamine-mPEG-2000 (mPEG-2000-DSPE),
were purchased from Avanti Polar Lipids. Cholesterol (CHO) was obtained
from Sigma-Aldrich. Melphalan was purchased from Dalian Meilun Biotechnology
Co., Ltd. Cell culture media and penicillin–streptomycin were
obtained from Corning Corporation (Corning). The hydrophobic fluorescent
dyes 3,3′-dioctadecyloxacarbo-cyanine perchlorate (DiO) and
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine
iodide (DiR) were purchased from Thermo Fisher Corporation.
Lin Z., Chu B., Qu Y., Wei X., Huang J., Wang F., Feng Y., Wang X., Luo H., Zhai X., Xu J., Liu X., Zhang L., Chen F., Wu Y, & Zheng Y. (2022). Liposome-Encapsulated Melphalan Exhibits Potent Antimyeloma Activity and Reduced Toxicity. ACS Omega, 8(1), 1693-1701.
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2

Formulation and Characterization of MEL Delivery

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MEL (4-hydroxy-2-methyl-n-(5-methyl-2-thiazolyl)-2H-benzothiazine-3-carboxamide-1,1-dioxide) as a model drug was obtained from EGIS Ltd. (Budapest, Hungary). HSA (lyophilized powder, purity >97%), Tween 80 (Tween), phosphate buffered saline (PBS) powder, disodium hydrogen phosphate (Na2HPO4), sodium dihydrogen phosphate (Na2HPO4), l-α-phosphatidylcholine (PC), and cholesterol (CHO) were purchased from Sigma Aldrich Co. Ltd. (Budapest, Hungary). The analytical grade solvents acetonitrile (MeCN), dimethyl sulfoxide (DMSO), dodecane, formic acid, and trifluoroacetic acid (TFA) were purchased from Merck KGaA (Darmstadt, Germany). Piroxicam, the internal standard for the HPLC method, was purchased from the Alfa Aeasar Co. (Alfa Aeasar GmbH & Co. KG, Karlsruhe, Germany). In all experiments, distilled water was purified using the Millipore Milli-Q® (Merck Ltd., Budapest, Hungary) 140 Gradient Water Purification System.
Katona G., Balogh G.T., Dargó G., Gáspár R., Márki Á., Ducza E., Sztojkov-Ivanov A., Tömösi F., Kecskeméti G., Janáky T., Kiss T., Ambrus R., Pallagi E., Szabó-Révész P, & Csóka I. (2020). Development of Meloxicam-Human Serum Albumin Nanoparticles for Nose-to-Brain Delivery via Application of a Quality by Design Approach. Pharmaceutics, 12(2), 97.
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3

Lipid and Sterol Extraction Protocol

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l-α-Phosphatidylcholine (PC)
from soybean (≥90%) was provided by Alfa Aesar (Ward Hill,
Massachusetts, USA). Cholesterol (CHO) (≥99%), 3-palmitoyl-sn-glycerol (≥99%), vitamin D3 (cholecalciferol)
(≥98%), sucrose (≥99.5%), glucose (≥99.5%), sodium
chloride, and calcium chloride (≥99.5%) were purchased from
Sigma-Aldrich (St. Louis, MI, USA) as dried powders and used without
further purification. 4-Palmitamido-2,2,6,6-tetramethylpiperidine-1-oxyl
(4-palmitamido-TEMPO) was also obtained from Sigma-Aldrich (St. Louis,
MI, USA). All the other chemicals and reagents such as chloroform,
ethanol, and methanol used were of analytical grade.
Ghiasi F., Eskandari M.H., Golmakani M.T., Rubio R.G, & Ortega F. (2021). Build-Up of a 3D Organogel Network within the Bilayer Shell of Nanoliposomes. A Novel Delivery System for Vitamin D3: Preparation, Characterization, and Physicochemical Stability. Journal of Agricultural and Food Chemistry, 69(8), 2585-2594.
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4

Liposomal Formulation Development

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l-α-Phosphatidylcholine (PC) from soybean, type II-S, 14–23% choline basis (CAS number 8002-43-5), cholesterol (CHO) (CAS number 57-88-5), chitosan (CAS number 9012-76-4; medium molecular weight and 75–85% deacetylated), ethanol of analytical grade (CAS number 64-17-5), glacial acetic acid (CAS number 64-19-7) and Triton X-100 (CAS number 9002-93-1) were purchased from Sigma–Aldrich (Milan, Italy). All the materials were used as acquired.
Bochicchio S., Dalmoro A., Bertoncin P., Lamberti G., Moustafine R.I, & Barba A.A. (2018). Design and production of hybrid nanoparticles with polymeric-lipid shell–core structures: conventional and next-generation approaches. RSC Advances, 8(60), 34614-34624.
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5

Soybean Lecithin Phosphatidylcholine Formulation

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Soybean lecithin (SBL) phosphatidylcholine was purchased from a local market (Nuevo León, México). Figure 8 illustrates the structural representation of SBL phosphatidylcholine along with its structural backbone entities. Cholesterol (Cho) was obtained from Sigma Aldrich (Darmsdat, Germany). Oregano essential oil (OEO) was purchased from PACALI (Nuevo León, México). Tween 80® was obtained from Sigma Aldrich (Darmsdat, Germany). Chloroform was purchased from J.T.Baker™ (Bridgewater, NJ, USA) and phosphate-buffered saline (PBS) solution was obtained from ThermoFisher (Waltham, MA, USA).
Aguilar-Pérez K.M., Medina D.I., Narayanan J., Parra-Saldívar R, & Iqbal H.M. (2021). Synthesis and Nano-Sized Characterization of Bioactive Oregano Essential Oil Molecule-Loaded Small Unilamellar Nanoliposomes with Antifungal Potentialities. Molecules, 26(10), 2880.
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6

Lipid Nanoparticle Formulation Development

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Soy phosphatidylcholine (PC; Chemical Abstracts Service [CAS] number 97281-47-5; Phospholipon® 90G) was purchased from Lipoid, Ludwigshafen, Germany. MJ (CAS number 24851-98-7), cholesterol (CHO; CAS number 57-88-5), and sodium oleate (SO; CAS number 143-19-1), were purchased from Sigma-Aldrich, St Louis, MO, USA. Tris(hydroxymethyl) aminomethane (CAS number 77-86-1) was purchased from Merck, Darmstadt, Germany. Polyoxyethylene glycerol tri-hydroxystearate 40 (Eumulgin® HRE 40 [EU], CAS number 61788-85-0) was purchased from Pharma Special, São Paulo, Brazil. Water was purified in a Milli-Q Plus purification system (Millipore, Billerica, MA, USA), and its resistivity was 18.2 MΩ-cm. All other chemicals and solvents used in this work were of analytical grade or better.
da Silva G.B., Scarpa M.V., Rossanezi G., do Egito E.S, & de Oliveira A.G. (2014). Development and characterization of biocompatible isotropic and anisotropic oil-in-water colloidal dispersions as a new delivery system for methyl dihydrojasmonate antitumor drug. International Journal of Nanomedicine, 9, 867-876.
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7

Liposomal Formulation of Green Tea Antioxidant

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The substances used for liposomal preparation were: Epigallocatechin-gallate (EGCG) derived from green tea (Sigma-Aldrich, Steinheim, Germany); 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC): N-(carbonyl-methoxypolyethylenglycol-2000)-1,2-distearoylsn-glycero-3-phosphoethanolamine Na-salt (MPEG-2000-DSPE) (Lipoid GmbH, Ludwigshafen am Rhein, Germany); and cholesterol (CHO) obtained from sheep wool (Sigma-Aldrich, Steinheim, Germany). All other solvents and reactive substances were obtained from Sigma-Aldrich, Steinheim, Germany, and had an analytical degree of purity.
Bulboaca A.E., Boarescu P.M., Porfire A.S., Dogaru G., Barbalata C., Valeanu M., Munteanu C., Râjnoveanu R.M., Nicula C.A, & Stanescu I.C. (2020). The Effect of Nano-Epigallocatechin-Gallate on Oxidative Stress and Matrix Metalloproteinases in Experimental Diabetes Mellitus. Antioxidants, 9(2), 172.
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8

Optimized Lipid-Based Drug Delivery

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VRD was purchased from Jinlan Pharm-Drugs Technology Co., Ltd. (Hangzhou, People’s Republic of China). l-α-Phosphatidylcholine (soya 95%) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Hydroxypropyl methylcellulose (HPMC) and cholesterol (CHO) were purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). Potassium dihydrogen phosphate was procured from BDH Chemicals Ltd. (Poole, UK). All other chemicals and reagents used were of analytical grade.
, & Fahmy U.A. (2015). Nanoethosomal transdermal delivery of vardenafil for treatment of erectile dysfunction: optimization, characterization, and in vivo evaluation. Drug Design, Development and Therapy, 9, 6129-6137.
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9

Lipid-Based Nanocarrier Preparation

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L-a-Phosphatidylcholine (PC) from egg yolk (CAS n. 8002-43-5), Cholesterol (CHO) (CAS n. 57-88-5),L-aPhosphatidyl -DL glycerol sodium salt (PG) from egg yolk lecithin (over 99% pure), vitamin B12, B12, (CAS n. 68-19-9), a-tocopherol, TC (CAS n. 10191-41-0), ergocalciferol, D2, (CAS n. 50-14-6), potassium phosphate monobasic (CAS n.7778-77-0) and sodium hydroxide (CAS n. 1310-73-2) were purchased from Sigma Aldrich (Milan, Italy) as dried powders and used without further purification. All the other chemicals and reagents such as chloroform (CAS n. 67-66-3), ethanol (CAS n. 64-17-5), methanol (CAS n. 67-56-1) (Sigma Aldrich, Milan, Italy) used were of analytical grade.
Bochicchio S., Barba A.A., Grassi G, & Lamberti G. (2016). Vitamin delivery: Carriers based on nanoliposomes produced via ultrasonic irradiation. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 69.
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