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Cd19 bv605 6d5

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CD19-BV605 (6D5) is a fluorochrome-conjugated antibody that recognizes the CD19 antigen. CD19 is a cell surface molecule expressed on B cells and some B cell malignancies. The BV605 fluorochrome can be used in multicolor flow cytometry applications.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Fortessa instrument, by BD (1 mentions) Cd11b bv650, by BioLegend (1 mentions) Cd4 pe cy7 rm4 4, by BioLegend (1 mentions) Ly6g apc cy7, by BioLegend (1 mentions) Cd8 buv395, by BD (1 mentions) Cd11c bv510, by BioLegend (1 mentions) Cd64 percpcy5, by BioLegend (1 mentions) Siglecf af647, by BD (1 mentions) Cd3 bv785, by BD (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Streptavidin bv605, by BioLegend (1 mentions) Protein labeling kit, by Thermo Fisher Scientific (1 mentions) Cd3 apc cy7 17a2, by BioLegend (1 mentions) Cd19 pe 6d5, by BioLegend (1 mentions) Cd4 apc cy7 gk1.5, by BioLegend (1 mentions) B220 pe ra3 6b2, by BioLegend (1 mentions) Cd8 percpcy5.5 53 6 7, by BioLegend (1 mentions) Cd138 apc 281 2, by BD (1 mentions)

Spelling variants of this product

CD19 (6D5; (32 citations) CD19-BV605 (19 citations)

2 protocols using cd19 bv605 6d5

1

Flow Cytometry Analysis of Immune Cells

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Single cell suspensions of blood (by cardiac puncture) were collected from NRL-CV, NRL and wild-type adult male and female mice and stained for flow cytometry. Antibodies used: CD3-BV785 (17A2, BD Biosciences, cat 564010), CD45-BV711 (30-F11, Biolegend, cat 103147), CD4-PE-Cy7 (RM4–4, Biolegend, cat 116016), CD8-BUV395 (53–6.7, BD Bioscience, cat 563786), CD19-BV605 (6D5, Biolegend, cat 115540), NKp46-BUV737 (29A1.4, BD Bioscience, cat 612805), LY6G-APC-Cy7 (1A8, Biolegend, cat 127623), CD11b-BV650 (M1/70, Biolegend, cat 101259), SiglecF-AF647 (E50–2440, BD Bioscience, cat 562680), CD64-PerCPCy5.5 (X54–5/7.1, Biolegend, cat 139308), TCRγδ-FITC (GL3, Biolegend, cat 118105), CD11c-BV510 (N418, Biolegend, cat 117353) and counterstained with DAPI (ThermoFisher, cat D1306). Samples were acquired on a BD Fortessa instrument. Data were analysed using FlowJo v10.8.1. For cell gating, RFP+ cells were subgated out of CD45+DAPI− single and the immune subsets were subgated out of the RFP+ gate (see Table 1 and Supplementary Figure 4a).
Dzien P., Raffo Iraolagoitia X., May S., Stevenson D., McGarry L., Soloviev D., Brown G., Nixon C., Kapeni C., De La Roche M., Blyth K., Lyons S., Bird T., Strathdee D., Fruhwirth G., Carlin L, & Lewis D. (2024). Multi-scale in vivo imaging of tumour development using a germline conditional triple-reporter system. Research Square.
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2

Comprehensive Multiparameter Flow Cytometry

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The following anti-mouse antibodies were used for FACS: CD19-PE (6D5), CD19-BV605 (6D5), CD3-AlexaFluor700 (17A2), CD3-APCCY7 (17A2), CD4-APC-Cy7 (GK1.5), CD8-PerCP-Cy5.5 (53-6.7), CD8-FITC (5H10-1), Streptavidin BV605, PD-1-BV421 (29F.1A12), PDL-1-Pecy7 (10F.9G2), Bcl6-PE-Dazzle594 (7D1), ICOS-AlexaFluor488 (C398.4A), CD45.1- BV605 (A20), B220-APCCy7 (RA3-6B2), Live Dead Aqua 430 were from Biolegend; CD19-AlexaFluor700 (1D3), CD19-FITC (1D3), IgD-FITC (11-26c), CD8-PECy7 (53-6.7), TNF-APC (MP6-XT22), PD-L1-PE (MIH5) and were from eBioscience; CD138-BV605 (281-2), CD138-APC (281-2), CD138-PE (281-2), CD138- Biotin, CD38-BV421 (90/CD38), CXCR5-Biotin (2G8), IFN-γ-FITC (XMG1.2), Ki-67-AlexaFluor647 (B56), Blimp-1-PE-CF594 (5E7), and CD8-BUV805 (53-6.7) were from BD Biosciences.
The following anti-mouse antibodies were used for tissue immunofluorescence: B220-PE (RA3-6B2) and CD3 FITC anti-CD3 (145-2C11) from Biolegend and CD138-APC (281-2) from BD Biosciences.
HEL was conjugated with AlexaFluor647 with a protein labeling kit (Invitrogen).
Serrán M.G., Vernengo F.F., Almada L., Beccaria C.G., Gazzoni Y., Canete P.F., Roco J.A., Boari J.T., Ramello M.C., Wehrens E., Cai Y., Zuniga E.I., Montes C.L., Cockburn I.A., Rodriguez E.V., Vinuesa C.G, & Gruppi A. (2022). Extrafollicular Plasmablasts Present in the Acute Phase of Infections Express High Levels of PD-L1 and Are Able to Limit T Cell Response. Frontiers in Immunology, 13, 828734.
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