Anti phospho ser3 cofilin1 antibody 3311s

Manufactured by Cell Signaling Technology

Anti-phospho-(Ser3)-Cofilin1 antibody (3311S) is a lab equipment product from Cell Signaling Technology. It is an antibody that specifically recognizes the phosphorylated form of the Cofilin1 protein at serine 3.

Automatically generated - may contain errors

Lab products found in correlation

Human α thrombin, by Enzyme Research (2 mentions) Diethylaminoethyl deae dextran, by Merck Group (2 mentions) Plasmid maxi kit, by Qiagen (2 mentions) β actin sc 47778, by Santa Cruz Biotechnology (2 mentions) Antibodies to ve cadherin, by Abcam (1 mentions) Anti gapdh antibody sc 32233, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Anti-cofilin1 (2 citations)

2 protocols using anti phospho ser3 cofilin1 antibody 3311s

Thrombin-Induced Signaling Pathway Analysis

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Human α-thrombin was purchased from Enzyme Research Laboratories (South Bend, IN). Anti-ATG7, anti-phospho-(Ser536)-RelA/p65 (3033S), anti-phospho-(Ser3)-Cofilin1 antibody (3311S), and anti-Cofilin1 (clone D3F9, 5175S) antibodies were purchased from Cell Signaling Technology (Beverly, MA). Antibodies to RelA/p65 (SC-8008), IκBα (SC-371), and β-actin (SC-47778) were from Santa Cruz Biotechnology (Santa Cruz, CA). An anti-TBP antibody was purchased from Abcam (AB33168, Cambridge, MA). Plasmid maxi kit was from QIAGEN Inc. (Valencia, CA) and diethylaminoethyl (DEAE)-dextran was obtained from Sigma-Aldrich Chemical Company (St. Louis, MO). All other materials were obtained from Thermo Fisher Scientific (Waltham, MA).

Shadab M., Millar M.W., Slavin S.A., Leonard A., Fazal F, & Rahman A. (2020). Autophagy protein ATG7 is a critical regulator of endothelial cell inflammation and permeability. Scientific Reports, 10, 13708.

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Antibody Characterization for Cell Signaling

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Human α-thrombin was purchased from Enzyme Research Laboratories (South Bend, IN). Diethylaminoethyl (DEAE)-dextran was obtained from Sigma-Aldrich Chemical Company (St. Louis, MO). A rabbit polyclonal anti-Beclin1 antibody (3738S) was purchased from Cell Signaling Technology (Beverly, MA). Polyclonal antibodies to VCAM-1 (SC-8304), RelA/p65 (SC-8008), IκBα (SC-371), and β-actin (SC-47778) were from Santa Cruz Biotechnology (Santa Cruz, CA). An anti-phospho-(Ser⁵³⁶)-RelA/p65 (3033S) was from Cell Signaling Technology (Beverly, MA). Antibodies to VE-cadherin were obtained from Abcam (AB33168, Cambridge, MA) and BD Biosciences (BD555661, San Jose, CA). A rabbit polyclonal anti-Cofilin1 (clone D3F9, 5175S) antibody and a rabbit polyclonal anti-phospho-(Ser³)-Cofilin1 antibody (3311S) were obtained from Cell Signaling Technology (Beverly, MA). An anti-GAPDH antibody (SC-32233) and an anti-Lamin B antibody (SC-6216) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Plasmid maxi kit was from QIAGEN Inc. (Valencia, CA). All other materials were purchased from Thermo Fisher Scientific (Waltham, MA).

Leonard A., Millar M.W., Slavin S.A., Bijli K.M., Santos D.A., Dean D.A., Fazal F, & Rahman A. (2019). Critical Role of Autophagy Regulator Beclin1 in Endothelial Cell Inflammation and Barrier Disruption. Cellular signalling, 61, 120-129.

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