At the jointing-booting stage, the second internode stems of DPW and HPW treated with different photoperiods, under growing seasons and sprayed with phytohormones were fixed in Carnoy's fixative I (3 parts of 95% ethanol to 1 part of glacial acetic acid). Sections (cross and longitudinal) with 10-μm-thick were cut on a cryostat microtome (Leica, CM1850, UV) with 1% agarose gel. After removing agarose gel using QIAquick Gel Extraction Kit (Qiagen, Cat. #: 28706, USA), the sections were stained with 1% safranin O (Solarbio) in water for 3 hours, rinsed three times in 85% ethanol for 10 minutes, and then counterstained with an alcoholic solution of fast green FCF (Solarbio, Cat. #: 907A035) (1 mg fast green FCF in 200 ml 50% ethanol) for 20 seconds. Images were captured using a Photometrics SenSys CCD camera on an Olympus BX-51 microscope (Olympus, Tokyo, Japan).
Fast green fcf
Manufactured by Solarbio
Sourced in China, United States
Fast Green FCF is a synthetic dye commonly used as a coloring agent in various laboratory and industrial applications. It is a water-soluble, green-colored powder that is widely used for staining and visualization purposes in various scientific and technical fields.
Automatically generated - may contain errors
Lab products found in correlation
Safranin o, by Solarbio (2 mentions)
Rotary microtome, by Leica (1 mentions)
Sensys ccd camera, by Olympus (1 mentions)
Cm1850, by Leica (1 mentions)
Qiaquick gel extraction kit, by Qiagen (1 mentions)
Bx51 microscope, by Olympus (1 mentions)
Aggrecan, by Abcam (1 mentions)
Mmp13, by Abcam (1 mentions)
Anti collagen 2, by Abcam (1 mentions)
Anti p65, by Cell Signaling Technology (1 mentions)
Lamin b1, by Cell Signaling Technology (1 mentions)
Anti inos, by Proteintech (1 mentions)
Type 2 collagenase, by Solarbio (1 mentions)
Adamts5, by Abcam (1 mentions)
Gapdh, by Proteintech (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Hematoxylin, by Solarbio (1 mentions)
Cox 2, by Proteintech (1 mentions)
3 protocols using fast green fcf
1
Microscopic Analysis of Internode Development
2
Localization of Starch Grains in Plant Shoots
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After fixing in the FAA solution, the samples were cut into 7-μm-thick paraffin sections with a rotary microtome (Leica, Wetzlar, Germany). Starch grains in the shoots were localized with a periodic acid–Schiff (PAS) reaction (Chu, 1963 (link)). The paraffin sections were soaked in 0.5% KIO4 for 10min, followed by 30min in a Schiff reagent, then dehydrated in a graded series of ethanol, and stained with Fast Green FCF (Ameresco 0689; Solarbio Life Science, Beijing, China). The sections were observed under a light microscope (E400; Nikon, Tokyo, Japan).
3
Chondrocyte Inflammatory Signaling Pathway
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Limonin (purity ≥ 98%), DMSO, Safranin O, collagenase type-II, Fast Green FCF, and hematoxylin were provided by Solarbio (Beijing, China). Recombinant rat IL-1β was purchased from Multi Sciences Biotech, Co., Ltd. (Hangzhou, China). Anti-Collagen II, Aggrecan, ADAMTS5, and MMP13 were purchased from Abcam (Cambridge, UK). Anti-p65, IκBα, and Lamin-B1 were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-INOS, COX-2, HO-1, NQO1, SOD1, Nrf2, and GAPDH were purchased from Proteintech (Wuhan, China). Reagents for cell culturing were purchased from Gibco (Grand Island, USA).
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