Sh-LINC00504 and its control (sh-NC) were transfected into SKBR3 and MDA-BM-231 cells for 48 h. Then, the cells were lysed in precooled ethanol (75%) and incubated at 4°C for 4 h. Next, cold PBS was used to wash the cells, followed by staining with BD PharmingenTM PI/RNase for 30 mins at room temperature. Finally, the cells in different cell cycle phases (G0/G1, S, and G2) were analysed by flow cytometry. A total of 10,000 cells were measured for each sample.
Pharmingen pi rnase
Manufactured by BD
The BD Pharmingen™ PI/RNase is a laboratory instrument designed for the detection and analysis of apoptosis, cell proliferation, and cell cycle progression. The product provides a standardized method for the simultaneous measurement of propidium iodide (PI) and ribonuclease (RNase) levels in cellular samples.
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Lab products found in correlation
5 protocols using pharmingen pi rnase
1
Cell Cycle Analysis of LINC00504 Knockdown
2
Cell Cycle Analysis by Flow Cytometry
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After transfection, cells were harvested and adjusted to the concentration of 1×106 cell/mL. Then pre-cold ethanol (75%) was used to dissolve the cells, followed by incubation at 4°C for 4h. After that, cells were washed with cold PBS again. Next, cells were stained with BD Pharmingen TM PI/RNase for 30 min at room temperature, followed by flow cytometer at different cell cycle phase (G1, S, and G2). 10000 cells were measured for each sample.
3
Cell Cycle Analysis by Flow Cytometry
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After transfection, cells were harvested and adjusted to the concentration of 1×106 cell/mL. Then pre-cold ethanol (75%) was used to dissolve the cells, followed by incubation at 4°C for 4h. After that, cells were washed with cold PBS again. Next, cells were stained with BD Pharmingen TM PI/RNase for 30 min at room temperature, followed by flow cytometer at different cell cycle phase (G1, S, and G2). 10000 cells were measured for each sample.
4
Cell Cycle Distribution Analysis Protocol
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Cell cycle distribution was assessed as previously described (23 (link)). Briefly, OSCC cells (5×105) were harvested, fixed with 75% ethanol on ice for 20 min, permeabilized with 0.25% Triton X-100 and stained with Pharmingen PI/RNase (BD Biosciences). After incubation for 15 min at 4°C, cells were analyzed using a flow cytometer (BD FACSAria III; BD Biosciences) and ModFit (version 3.0; Verity Software House, Inc.). The data were quantified using FlowJo software (version 3.0; FlowJo, LLC).
5
Cell Cycle Analysis of NSCLC Cells
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Cell cycle distribution was investigated according to a recent report (10 (link)). NSCLC cells were harvested, fixed, permeabilized, and stained with Pharmingen PI/RNase (BD). After incubation for 15 min, cells were analyzed using ModFit and the data were quantified by FlowJo software (BD).
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