Primary antibodies used included rat anti-human Ki-67 (DakoCytomation, Carpinteria, CA), D2-40 anti-podoplanin (clone D2-40, Dako, catalog number M3619) and anti-CD31 (rabbit polyclonal, Spring Bioscience, catalog number E11114). The appropriate secondary antibodies were linked to an identifying chromogen.
D2 40 anti podoplanin clone d2 40
Manufactured by Agilent Technologies
The D2-40 anti-podoplanin (clone D2-40) is a laboratory reagent used for the detection of podoplanin, a transmembrane glycoprotein, in various tissue samples. This monoclonal antibody can be used in immunohistochemical procedures to identify cells expressing podoplanin.
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Lab products found in correlation
2 protocols using d2 40 anti podoplanin clone d2 40
1
Immunohistochemical Staining of Ki-67, Podoplanin, and CD31
2
Quantifying Tumor Microenvironment Features
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Primary antibodies used included rat anti-mouse Ki-67 (DakoCytomation, Carpinteria, CA), D2-40 antipodoplanin (clone D2-40, Dako, catalog number M3619) and anti-CD31 (rabbit polyclonal, Spring Bioscience, catalog number E11114). The appropriate secondary antibodies were linked to an identifying chromogen.
Imaging Analysis and Quantitation of Ki-67 Index, Mitotic Count and Lymphovascular Tumor Emboli (Embolic Density, Shape and Size) ERAs applied to each TMA core were successful in recognizing tumor emboli based on the Gaussian kernel and other imaging techniques. SRAs imaged and quantitated nuclear Ki-67 immunoreactivity. 22 Mitotic algorithms recognized and identi ed the various stages of mitosis including prophase, metaphase and telophase. 26 Speci c circumferential lymphovascular immunoreactivities were detected and analyzed by the appropriate SRAs which included membrane recognition and cytoplasmic recognition algorithms whose e cacy had been demonstrated previously. 25 ImageJ was used to measure both the shape (elongation ratio) as well as the size (perimeter) of the emboli. 28 Experimental Studies
Imaging Analysis and Quantitation of Ki-67 Index, Mitotic Count and Lymphovascular Tumor Emboli (Embolic Density, Shape and Size) ERAs applied to each TMA core were successful in recognizing tumor emboli based on the Gaussian kernel and other imaging techniques. SRAs imaged and quantitated nuclear Ki-67 immunoreactivity. 22 Mitotic algorithms recognized and identi ed the various stages of mitosis including prophase, metaphase and telophase. 26 Speci c circumferential lymphovascular immunoreactivities were detected and analyzed by the appropriate SRAs which included membrane recognition and cytoplasmic recognition algorithms whose e cacy had been demonstrated previously. 25 ImageJ was used to measure both the shape (elongation ratio) as well as the size (perimeter) of the emboli. 28 Experimental Studies
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