Day 1 adult worms, unless otherwise indicated, were collected and sonicated in RIPA buffer (100 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100, 1% deoxycholic acid, 0.1% SDS, 5 mM EDTA, 10 mM NaF) containing 1 mM DTT and proteinase inhibitor (Sigma) before boiling and loading. Antibodies against acetyl-histone H4 (Millipore), phospho-p38 (Promega) and tubulin (Sigma) were used.
Phospho p38
Manufactured by Promega
Phospho-p38 is a lab equipment product that measures the phosphorylation of the p38 MAPK protein. It provides a quantitative assessment of the activation state of the p38 MAPK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Proteinase inhibitor, by Merck Group (1 mentions)
Acetyl histone h4, by Merck Group (1 mentions)
Total cdk2, by Cell Signaling Technology (1 mentions)
Total cdc2, by Cell Signaling Technology (1 mentions)
Total erk1 2, by Promega (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Phosphor cdc2, by Cell Signaling Technology (1 mentions)
Phosphor jnk1 2, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
2 protocols using phospho p38
1
Protein Extraction and Western Blot
2
Western Blot Analysis of Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Western blot analysis of whole-cell lysates was conducted as previously described [17 (link)]. The blots were probed with the following primary antibodies: phosphor-CDK2, total CDK2, CDK3, phosphor-CDC2, total CDC2, phospho-ERK1/2, phosphor-JNK1/2 (all from Cell Signaling), CDK4 (Santa Cruz Biotechnology), total p38, total ERK1/2, total JNK1/2, phospho-p38, total p38 MAPK (all from Promega), anti α-tubulin (Sigma) and anti-LC3 (MBL). Immunoreactive proteins were detected as previously described [17 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!