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Anti ago2 monoclonal antibodies

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Anti-Ago2 monoclonal antibodies are laboratory reagents used to detect and study the Ago2 protein, which is a key component of the RNA-induced silencing complex (RISC). These antibodies can be used in various immunoassays and research applications to investigate Ago2 expression and function.

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Lab products found in correlation

Anti his monoclonal antibody, by Cell Signaling Technology (2 mentions)

2 protocols using anti ago2 monoclonal antibodies

1

eIF1A Interaction with Ago2 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli expressed GST-tagged human eIF1AX (known as eIF1A), or globular domain (GD, 25 ~114aa), or N-tail deletion (ND, 25~144aa) or C-tail deletion (CD, 1~114aa) were purified by GST-pull down assays (#88822, Thermo Scientific) with glutathione magnetic beads from pre-cleared cell lysates of HEK293 cells stably expressing HA-Ago2 in the presence of 10μg/ml Ribonuclease A treatment. Western blot assays were performed with anti-Ago2 monoclonal antibodies (Abcam, ab57113, 1:1000 dilution). For the Ago2 domain screening assays, E. coli expressed His-tagged L1, PAZ, sumo-MID, PIWI domains and Sumo, which were purified by Ni-NTA resin, were performed GST-pull down assays with E. coli expressed GST-eIF1A. Western blot assays were performed with anti-His monoclonal antibodies (Cell Signaling, #2366S, - 1:1000 dilution).
Yi T., Arthanari H., Akabayov B., Song H., Papadopoulos E., Qi H.H., Jedrychowski M., Güttler T., Guo C., Luna R.E., Gygi S.P., Huang S.A, & Wagner G. (2015). eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nature communications, 6, 7194.
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2

eIF1A Interaction with Ago2 Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
E. coli expressed GST-tagged human eIF1AX (known as eIF1A), or globular domain (GD, 25 ~114aa), or N-tail deletion (ND, 25~144aa) or C-tail deletion (CD, 1~114aa) were purified by GST-pull down assays (#88822, Thermo Scientific) with glutathione magnetic beads from pre-cleared cell lysates of HEK293 cells stably expressing HA-Ago2 in the presence of 10μg/ml Ribonuclease A treatment. Western blot assays were performed with anti-Ago2 monoclonal antibodies (Abcam, ab57113, 1:1000 dilution). For the Ago2 domain screening assays, E. coli expressed His-tagged L1, PAZ, sumo-MID, PIWI domains and Sumo, which were purified by Ni-NTA resin, were performed GST-pull down assays with E. coli expressed GST-eIF1A. Western blot assays were performed with anti-His monoclonal antibodies (Cell Signaling, #2366S, - 1:1000 dilution).
Yi T., Arthanari H., Akabayov B., Song H., Papadopoulos E., Qi H.H., Jedrychowski M., Güttler T., Guo C., Luna R.E., Gygi S.P., Huang S.A, & Wagner G. (2015). eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nature communications, 6, 7194.
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