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Dual pam 100 spectrometer

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The Dual-PAM-100 is a spectrometer designed for analyzing and measuring the fluorescence of samples. It is capable of simultaneously recording two independent fluorescence signals. The device provides accurate data on the photosynthetic parameters of the analyzed samples.

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Lab products found in correlation

P515 535 accessory module, by Walz (1 mentions)

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Dual-PAM-100 (172 citations) Dual-PAM (16 citations)

4 protocols using dual pam 100 spectrometer

1

Plant Growth and Photosynthesis Analysis

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The plants were first grown under LD-GL conditions (16-h light/8-h dark cycle, 60 μmol photons m−2·s−1, 22 °C) for 7 d and then transferred to the experimental conditions described in each figure legend. FW was determined using plants above ground. Chlorophyll was extracted from frozen leaves with 80% (vol/vol) acetone, and chlorophyll content was determined through spectrophotometry in accordance with previous research (6 (link)). Photosynthetic parameters, such as Fv/Fm, Y(I), Y(NA), Y(ND), Y(II), Y(NPQ), and Y(NO), were determined by measuring the chlorophyll-a fluorescence and P700 state with a Dual-PAM-100 spectrometer (Walz).
Yokochi Y., Yoshida K., Hahn F., Miyagi A., Wakabayashi K.I., Kawai-Yamada M., Weber A.P, & Hisabori T. (2021). Redox regulation of NADP-malate dehydrogenase is vital for land plants under fluctuating light environment. Proceedings of the National Academy of Sciences of the United States of America, 118(6), e2016903118.
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2

Evaluating Photosynthetic Responses in Plants

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Using plants grown for 4 wk before being dark-adapted for 8 h, Fv/Fm and NPQ were measured with a Dual-PAM-100 spectrometer (Walz). NPQ was determined after illumination for 3 min with 70 μmol photons m−2·s−1 light intensity. After the measurement of these parameters, the FW of the plant above ground and chlorophyll content in rosette leaves were measured. Leaves were frozen in liquid nitrogen and then chlorophyll was extracted with 80% (vol/vol) acetone. Using spectrophotometry, chlorophyll content was determined as the sum of the contents of chlorophyll a and b according to previously published methods (22 (link), 47 ).
Yokochi Y., Fukushi Y., Wakabayashi K.I., Yoshida K, & Hisabori T. (2021). Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 118(51), e2114952118.
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3

Chlorophyll Fluorescence Dynamics in Plants

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Plants grown at a light intensity of 60 µmol photons m−2 s−1 were dark-adapted for 8 h, after which Fv/Fm, Y(II), and NPQ were measured using a Dual-PAM-100 spectrometer (Walz, Heinz, Germany). The time courses of Y(II) and NPQ were measured with actinic red light at 60 µmol photons m−2 s−1 for 8 min, while recovery in darkness was recorded for 8 min. Saturating pulses of red light were applied at 6000 µmol photons m−2 s−1 at 0.4-s durations. Y(II) and NPQ were calculated on the DUAL-PAM-100 software using previously applied equations (Kramer et al. 2004 (link)).
Fukushi Y., Yokochi Y., Hisabori T, & Yoshida K. (2024). Overexpression of thioredoxin-like protein ACHT2 leads to negative feedback control of photosynthesis in Arabidopsis thaliana. Journal of Plant Research, 137(3), 445-453.
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4

Measuring Proton Motive Force and ATP Synthase Conductivity

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To measure the magnitude and kinetics of pmf formation and to determine the conductivity of the ATP synthase to protons, changes in the ECS (P515) signal were recorded with a Dual‐PAM‐100 spectrometer and its P515/535 accessory module (Walz, Effeltrich, Germany) (Schreiber and Klughammer, 2008; Klughammer et al., 2013). A measuring light at a 2000‐Hz pulse frequency was used in all ECS measurements as described in Nikkanen et al. (2018). Dark intervals of 250 msec were applied at time‐points specified in Figure S5 to determine the magnitude of light‐induced pmf (ECST), and to calculate the conductivity parameters of the ATP synthase (gH+) as well as the proton flux parameter (vH+), as described previously (Cruz et al., 2001; Cruz et al., 2005; Nikkanen et al., 2018).
Guinea Diaz M., Nikkanen L., Himanen K., Toivola J, & Rintamäki E. (2020). Two chloroplast thioredoxin systems differentially modulate photosynthesis in Arabidopsis depending on light intensity and leaf age. The Plant Journal, 104(3), 718-734.
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