Images were obtained with a Nikon SMZ1500 microscope or a Nikon inverted A1r spectral confocal microscope. Z stacks were made through the entire CHT during confocal imaging in multiple fluorescent channels. Immunofluorescence double staining was carried out and analyzed as described previously (Gao et al., 2015 (link)), with chicken anti-GFP (1:400, Life Technologies, catalog no. A10262) and rabbit anti-phospho-histone 3 (pH3) antibodies (1:250, abcam, catalog no. ab5176). AlexaFluor488-conjugated anti-chicken secondary antibody (1:1,000, Life Technologies, catalog no A11039) and AlexaFluor594-conjugated anti-rabbit secondary antibody (1:1,000, Life Technologies, catalog no. A11012) were used to reveal primary antibodies.
Alexafluor488 conjugated anti chicken secondary antibody
Manufactured by Thermo Fisher Scientific
The AlexaFluor488-conjugated anti-chicken secondary antibody is a fluorescently-labeled antibody that binds to primary antibodies raised against chicken antigens. It is designed for use in immunodetection and imaging applications.
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Lab products found in correlation
Alexa fluor 594 conjugated anti rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions)
Chicken anti gfp, by Thermo Fisher Scientific (2 mentions)
Inverted a1r spectral confocal microscope, by Nikon (1 mentions)
Smz1500 microscope, by Nikon (1 mentions)
Rabbit anti phospho histone3 ph3 antibodies, by Abcam (1 mentions)
Inverted a1r spectral microscope, by Nikon (1 mentions)
2 protocols using alexafluor488 conjugated anti chicken secondary antibody
1
Fluorescent Imaging of Cell Cycle Markers
2
Fluorescent Embryo Imaging Protocol
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Transgenic fluorescent embryos were embedded in 1% agarose in a glass-bottom dish. Immunofluorescence double staining was performed as described previously57 (link), with chicken anti-GFP (1:400; Life Technologies) and rabbit anti-phospho-histone 3 (pH3) antibodies (1:250; Abcam). AlexaFluor488-conjugated anti-chicken secondary antibody (1:1000; Life Technologies) and AlexaFluor594-conjugated anti-rabbit secondary antibody (1:1000; Life Technologies) were subsequently utilized to reveal primary antibodies. Confocal imaging was performed using a Nikon inverted A1r spectral microscope.
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