Ps 1145

Manufactured by Santa Cruz Biotechnology

Sourced in United States

PS-1145 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed to perform a core function, but a detailed unbiased and factual description cannot be provided without the risk of extrapolation or interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Human insulin, by Merck Group (2 mentions) Pkc θ sirna, by Santa Cruz Biotechnology (2 mentions) Ikkβ sirna, by Santa Cruz Biotechnology (2 mentions) Hypochlorous sodium solution, by Merck Group (2 mentions) Antibody against phospho irs 1 tyr612, by Merck Group (2 mentions) Jnk2 sirna, by Santa Cruz Biotechnology (2 mentions) Protein a g agarose, by Santa Cruz Biotechnology (2 mentions) Control sirna, by Santa Cruz Biotechnology (2 mentions) Ripa lysis buffer, by Santa Cruz Biotechnology (2 mentions) Mouse 3t3 l1 preadipocytes, by American Type Culture Collection (2 mentions) Epilife medium, by Thermo Fisher Scientific (1 mentions) F 12k nutrient mixture, by Thermo Fisher Scientific (1 mentions) Defined keratinocyte sfm, by Thermo Fisher Scientific (1 mentions) Poly 1 c, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Glut4, by Cell Signaling Technology (1 mentions) Na k atpase, by Santa Cruz Biotechnology (1 mentions) Hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)

3 protocols using ps 1145

Culturing Diverse Human Cell Lines

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Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s minimal essential medium (DMEM, Gibco, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate (P/S). The EBV-positive nasopharyngeal epithelial (NPE) cell lines NP361hTERT-EBV and NP460hTERT-EBV were grown in a 1:1 mixture of Defined Keratinocyte-SFM (Gibco) and Epilife™ medium (Gibco) with 1% P/S. The EBV-positive NPC cell line C666-1 was grown in RPMI-1640 medium (Gibco) supplemented with 10% FBS and 1% P/S, and the EBV-positive gastric carcinoma cell line AGS-BX1 was cultured in F-12K Nutrient mixture (Gibco) supplemented with 10% FBS and 1% P/S. The cell lines NP460hTERT-EBV and AGS-BX1 were stimulated for 16 h with 0.5 µg/mL poly(I:C) (Invitrogen, Waltham, MA, USA), and NP361hTERT-EBV was stimulated for 16 h with 1 µg/mL poly(I:C) (Invitrogen). C666-1 cells were treated for 48 h with 0.2 mM PS-1145 (Santa Cruz Biotechnology, Dallas, TX, USA) to inhibit NF-κB activity. All cells were cultured at 37 °C with 5% CO₂.

Verhoeven R.J., Tong S., Zong J., Chen Y., Tsao S.W., Pan J, & Chen H. (2018). NF-κB Signaling Regulates Epstein–Barr Virus BamHI-Q-Driven EBNA1 Expression. Cancers, 10(4), 119.

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Insulin Signaling in 3T3-L1 Adipocytes

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Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC). [1, 2-³H] 2-deoxy-D-glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA). Hypochlorous sodium solution, human insulin, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), SP600125, and antibody against phospho-IRS-1 (Tyr612) were purchased from Sigma-Aldrich (St. Louis, MO). Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH and Na⁺/K⁺ ATPase were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against phospho-IRS-1 (Ser307), IRS-1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and horseradish peroxidase-linked secondary antibodies (Beverly, MA). ONOO⁻ was obtained from Calbiochem (Billerica, MA). All other chemicals were of the highest commercial grade available.

Zhou J., Wang Q., Ding Y, & Zou M.H. (2015). Hypochlorous acid via peroxynitrite activates protein kinase Cθ and insulin resistance in adipocytes. Journal of Molecular Endocrinology, 54(1), 25-37.

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Insulin Resistance in 3T3-L1 Adipocytes

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Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA, USA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC, USA). [1,2-³H] 2-deoxy-d-glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA, USA). Hypochlorous sodium solution, human insulin, Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME), SP600125, and antibody against phospho-IRS1 (Tyr612) were purchased from Sigma–Aldrich. Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH, and Na⁺/K⁺ ATPase were obtained from Santa Cruz Biotechnology, Inc. Antibodies against phospho-IRS1 (Ser307), IRS1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and HRP-linked secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ONOO⁻ was obtained from Calbiochem (Billerica, MA, USA). All other chemicals were of the highest commercial grade available.

Zhou J., Wang Q., Ding Y, & Zou M.H. (2015). Hypochlorous acid via peroxynitrite activates protein kinase Cθ and insulin resistance in adipocytes. Journal of Molecular Endocrinology, 54(1), 25-37.

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