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Monoclonal anti rac1

Manufactured by BD
Sourced in Japan

Monoclonal anti-Rac1 is a laboratory reagent used for the detection and analysis of the Rac1 protein. Rac1 is a small GTPase that plays a role in various cellular processes. This monoclonal antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to identify and study the Rac1 protein in biological samples.

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2 protocols using monoclonal anti rac1

1

Immunoblotting analysis of BCR signaling

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The following antibodies were used: polyclonal anti-BCR (Santa Cruz Biotechnology, sc-886, 1:1000), polyclonal anti-phospho-BCR Tyr-177 (Cell Signaling Technology, 3901, 1:1000), polyclonal anti-Tiam1 (IP, Santa Cruz Biotechnology, sc-872; Western blot, Bethyl, A300-099A, 1:1000), monoclonal anti-tubulin (Sigma, 1:5000), monoclonal anti-Rac1 (BD Biosciences, 610650, 1:1000), monoclonal anti-NR1 (Millipore, MAB1586, 1:500), monoclonal anti-Myc (Santa Cruz Biotechnology, sc-40, 1:400), and monoclonal anti-GFP (Neuromab, 75-131, 1:400).
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2

Antibody Immunoblotting Protocol

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The following antibodies were used: polyclonal anti-Dock8 from Takara Bio (Kyoto, Japan); monoclonal anti-Nck1, monoclonal anti-Rac1, and monoclonal anti-Cdc42 from BD Biosciences (Franklin Lakes, NJ, USA); monoclonal RhoA and monoclonal anti-GST tag from Santa Cruz Biotechnology (Santa Cruz, CA, USA); polyclonal anti-PDGF receptor alpha from Cell Signaling Technology (Danvers, MA, USA); polyclonal anti-p75NTR from Promega (Fitchburg, WI, USA); and monoclonal FLAG-tag, monoclonal HA-tag, and monoclonal anti-actin from MBL (Nagoya, Japan). Peroxidase-conjugated secondary antibodies were obtained from GE Healthcare (Fairfield, CT, USA) or Nacalai Tesque (Kyoto, Japan). Fluorescence-labeled secondary antibodies were obtained from Abcam (Cambridge, UK) or Life Technologies (Carlsbad, CA, USA).
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