The phenotype of effector T cell populations was determined by FACS analysis using antibodies described above, as well as mouse- antisheep or antibovine MHC class I (MCA2444GA; AbD Serotec), B-cell (MCA2443GA; AbD Serotec) and CD8 (MCA2216GA; AbD Serotec). Cells (2 × 105–1 × 106) were mixed with an equal volume of primary antibody (final concentration 1 μg ml-1), incubated at 4°C for 30 min, washed three times using PBS, and resuspended in 50 μl FACS medium (2% horse serum in PBS). FITC-labelled goat anti-mouse IgG (AbD Serotec) was used as secondary antibody. Cells were incubated at 4°C for 30 min, washed as before and resuspended in FACSFlow (Becton Dickinson Biosciences) for data acquisition and analysis using either a FACSCalibur (Becton Dickinson Biosciences) or a FACSAria (Becton Dickinson Biosciences).
Fitc labelled goat anti mouse igg
Manufactured by Bio-Rad
Sourced in United Kingdom
FITC-labelled goat anti-mouse IgG is a secondary antibody reagent designed for the detection of mouse immunoglobulin G (IgG) in various immunoassay applications. The antibody is conjugated with the fluorescent dye FITC (fluorescein isothiocyanate), which allows for the visualization and quantification of mouse IgG targets.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc labelled goat anti mouse igg
1
Phenotyping Effector T Cells by FACS
2
Enumeration of T-cell Subsets in PBMC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Enumeration of CD4+ and CD8+ T-cell subsets in PBMCs of the vaccinated and control groups was performed on FACS Calibur® instrument (BD Bioscience). 10 μL of anti-duck CD4 monoclonal antibody or anti-duck CD8 monoclonal antibody (AbD Serotec Ltd., UK) was added to the PBMC (5x105cells) in duplicates and incubated at 37 °C for 30 min. Fluorescein isothiocyanate (FITC)-labelled goat anti-mouse IgG (AbD Serotec Ltd., UK) was then added and incubated at 37 °C for 30 min. The cells were washed and resuspended with PBS and then subjected to flow cytometry analysis. Ten thousand cells were analysed in ‘Cell quest’ software of FACS Calibur (BD Bioscience).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!