Equivalent amounts of whole cell lysate were separated by 10% SDS-PAGE, and then transferred to a 0.45 μm nitrocellulose membrane. Membranes were blocked with 5% skim milk, and incubated overnight at 4 °C with the following antibodies: E-cadherin (BD Biosciences, San Jose, CA), cytokeratin-18 and cellular fibronectin (both from Sigma-Aldrich, Oakville, ON, Canada), vimentin, phospho-ERK1/2 mitogen activated protein kinase (MAPK), total-ERK1/2 MAPK, phospho-p38 MAPK, total-p38 MAPK, SLUG, SNAIL, or phospho-SMAD 2/3 (all from Cell Signaling, Danvers, MA). Membranes were then stripped and re-probed for the housekeeping protein, β-tubulin (Sigma-Aldrich, Oakville, ON, Canada).
Cytokeratin 18
Manufactured by Merck Group
Sourced in Canada
Cytokeratin-18 is a protein that is a member of the cytokeratin family. It is a structural component of the cytoskeleton in epithelial cells. Cytokeratin-18 is commonly used as a biomarker in various laboratory and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Vimentin, by Cell Signaling Technology (2 mentions)
E cadherin, by BD (2 mentions)
Cellular fibronectin, by Merck Group (1 mentions)
Phospho smad2 3, by Cell Signaling Technology (1 mentions)
Total p38 mapk, by Cell Signaling Technology (1 mentions)
Phospho erk1 2 mitogen activated protein kinase, by Cell Signaling Technology (1 mentions)
β tubulin, by Merck Group (1 mentions)
Snail, by Cell Signaling Technology (1 mentions)
Phospho p38 mapk, by Cell Signaling Technology (1 mentions)
Plakoglobin, by BD (1 mentions)
Iressa, by AstraZeneca (1 mentions)
Fibronectin, by Merck Group (1 mentions)
Fgfr1, by Santa Cruz Biotechnology (1 mentions)
Pd173074, by Pfizer (1 mentions)
Zd1839, by AstraZeneca (1 mentions)
Mk2206, by Bio-Techne (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
2 protocols using cytokeratin 18
1
Western Blot Analysis of Epithelial-Mesenchymal Markers
2
Epithelial-Mesenchymal Transition Signaling
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