Millipore super purity water system

Medium molecular weight (MMW) chitosan, high molecular weight (HMW) 80/1000 chitosan, and high molecular weight (HMW) 80/3000 chitosan were purchased from Sigma-Aldrich (Steinheim, Germany). Essential oil obtained from Boswellia serrata resin was obtained from “Kvapų namai”, Kaunas, Lithuania. Emulsifiers Tween 20^®, Tween 80^®, Span 20^®, and Span 80^® were purchased from Sigma-Aldrich (Germany). Acetic acid (100%) and sodium hydroxide were obtained from Sigma-Aldrich (Germany). Purified water for experiments was produced with a Millipore Super Purity Water System (Sigma-Aldrich Corp., St. Louis, MO, USA).

Dried Elsholtzia ciliata herb was purchased from “Zolynu namai” (Vilnius, Lithuania). A voucher specimen (L 17710) has been deposited at the Herbarium of the Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences.
HPLC eluents: formic acid, phosphoric acid, and acetonitrile were purchased from Sigma-Aldrich, (Steinheim, Germany), methanol from Carl Roth GmbH, (Karlsruhe, Germany). Standards for HPLC analysis: chlorogenic acid, rosmarinic acid, apigenin, and eugenol were purchased from Extrasynthese, (Genay, France). Wall material compounds: sodium caseinate, skim milk, maltodextrin, gum arabic, beta-cyclodextrin were purchased from Sigma-Aldrich, (Steinheim, Germany), resistant-maltodextrin (Promitor 85 ™) was purchased from Bang & Bonsomer, (Vilnius, Lithuania). Folin-Ciocalteu reagent, gallic acid monohydrate, and sodium carbonate were purchased from Sigma-Aldrich GmbH (Buchs, Switzerland). Extraction solvent ethanol (96%) was purchased from Vilniaus degtine, (Vilnius, Lithuania). Water used in HPLC and for the sample preparation was produced with a Millipore Super Purity Water System, (Sigma-Aldrich Corp., St. Louis, MO, USA).

Dried Origanum onites L. herb was obtained from “İnanTarım ECO DAB’’, Turkey. Voucher specimens (No. L170711) have been deposited at the Herbarium of the Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences. Carvacrol (>98%), HPLC eluent acetic acid (99.8%), as well as wall material gum arabic and β-Cyclodextrin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tween 20 was purchased from Scharlau Chemie S.A. (Barcelona, Spain) and bovine Gelatin bloom value 100 was purschased from Gelita AG. (Eberbach, Germany). HPLC eluent methanol was from Carl Roth GmbH (Karlsruhe, Germany) and rosmarinic acid (>98%) was from ChromaDex (Santa Ana, TX, USA). Extraction solvent ethanol (96% v/v) was purchased from Vilniaus degtinė (Vilnius, Lithuania). The purified water used in HPLC and for sample preparation was produced with a Millipore Super Purity Water System (Sigma-Aldrich Corp., St.Louis, MO, USA).