Images of brain sections stained with anti-Egr1 and DAPI were collected using a fluorescence microscope, Model IX70 (Olympus), coupled to a cooled CCD camera, C4742-95-12ERG (Hamamatsu Photonics). Brain structures were identified microscopically and in digital photos according to the Paxinos and Franklin’s mouse brain atlas33 . To count the number of Egr1+ neurons, Egr1-positive signals were distinguished from background by binarizing the images using a software, Adobe Photoshop.
C4742 95 12erg
Manufactured by Hamamatsu Photonics
The C4742-95-12ERG is a high-performance CCD camera from Hamamatsu Photonics. It features a 1344 x 1024 pixel image sensor with a 6.45 x 6.45 μm pixel size. The camera can capture images at a maximum frame rate of 12 frames per second. The camera has a USB 2.0 interface for data transfer and control.
Automatically generated - may contain errors
9 protocols using c4742 95 12erg
1
Quantifying Egr1+ Neurons in Mouse Brain
2
Immunostaining and Glomerular Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For fluorescent signals of immunostaining, digital images were captures with a digital CCD camera, C4742-95-12ERG (Hamamatsu Photonics). Tone was reversed and monochrome image was used for measurement. To quantitate the staining level of each glomerulus, the mean pixel intensity within the region surrounded by the periglomerular cell nuclei was measured using Scion Image (Scion Corp.). Expression levels of Pcdh21, Syn, and PSD95 in the glomeruli, were measured as average fluorescent intensities in the glomeruli and used for the normalization.
3
FRET Biosensors for Lyn-FAK and H3K9 Dynamics
4
Optical and Fluorescence Microscopy Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical images were photographed with an Olympus Optical AX70 microscope. Sections were also analysed by a fluorescence microscope, Olympus model IX70 equipped with a cooled CCD camera, C4742-95-12ERG (Hamamatsu Photonics). For quantification of signals, the tone was reversed and monochrome images were used. Staining intensities were measured with ImageJ. Statistical analyses were performed with Excel 2010 (Microsoft).
5
Quantifying Immunofluorescence Signals in the Olfactory Bulb
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For fluorescent signals of immunostaining, digital images were taken with a digital CCD camera, C4742-95-12ERG (Hamamatsu Photonics), or with two-photon microscopy (Olympus FV1000). Tone was reversed and a monochrome image was used for the measurement. For staining of the OB sections, digital images were taken with a digital CCD camera, Model DP70 (Olympus). To quantify the staining level of each glomerulus, the mean pixel intensity within the region surrounded by the periglomerular-cell nuclei was measured using Scion Image (Scion Corp.).
6
Immunohistochemical Analysis of Olfactory Bulb
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunohistochemistry, OB sections were perfused and treated with the primary and secondary antibodies9 (link). Sections were photographed with a fluorescence microscope, Model IX70 (Olympus), coupled to a cooled CCD camera, C4742-95-12ERG (Hamamatsu Photonics).
7
Fluorescent Imaging and Quantification of Olfactory Bulb
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For fluorescent signals of immunostaining, digital images were captured with a digital CCD camera, C4742-95-12ERG (Hamamatsu Photonics), and two-photon microscopy (Olympus). Tone was reversed and a monochrome image was used for the measurement. For staining of the OB sections, digital images were captured with a digital CCD camera, Model DP70 (Olympus). To quantitate the staining level of each glomerulus, the mean pixel intensity within the region surrounded by the periglomerular cell nuclei was measured using Scion Image (Scion Corp.).
8
Immunohistochemistry of OB Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunohistochemistry, OB sections were fixed with 4% paraformaldehyde in PBS and treated with the primary and secondary antibodies. The sections were then photographed with a fluorescence microscope, Model IX70 (Olympus), coupled to a cooled CCD camera, C4742-95-12ERG (Hamamatsu Photonics).
9
Characterization of PLOT Columns Using Coumarin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The LC measurements were carried out using an LPG-3400M pump (Thermo Fisher Scientific, Germering, DE), a Rheodyne 7125 manual injector with an 5 μL sample loop (IDEX Health & Science GmbH) with home-build T-split injection flow system, a fluorescence microscope IX-71 equipped with the U-RFT-T lamp power supply (Olympus, Tokyo, JP), and a charge-coupled device camera C4742-95-12ERG (Hamamatsu Photonics, Shizuoka, JP). The detailed measurement conditions were the same as in our previous study on 5 µm i.d.-PLOT columns (c.f. ref. [13] ). Coumarin compounds were utilized to examine the PLOT columns using 70:30% and 100:0% (v/v) methanol/water as mobile phase. The fluorescence microscope images were processed with MatLab 2012b software (Mathworks, MA, USA), to visualize the chromatograms. The residence time of the quasi-unretained marker needed to be determined in order to calculate the retention factors (k) for coumarins using the same procedure as in [13] (the detailed information is described in the SM).
SEM measurements on the fabricated PLOT columns were conducted according to the same procedure as in [13] to measure the capillary diameter and porous-silica layer thickness.
SEM measurements on the fabricated PLOT columns were conducted according to the same procedure as in [13] to measure the capillary diameter and porous-silica layer thickness.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!