Pinaacle 900t

The measurement of the contents of TMEs and mineral elements was performed as described in the study of Sleimi et al. [15 (link)]; briefly, mineralization was conducted in Teflon bombs for 2 h at 110 °C. An amount of 45 mg of fresh plant material was extracted in a mix of acids (HNO₃/H₂SO₄/HClO₄; at the rate 10:1:0.5; v/v/v). The obtained extracts were diluted in 0.5% nitric acid, and finally filtered to measure the Al, Ba, K, Ca, Mg, Zn and Fe contents in the plant tissues using atomic absorption spectrometry (Perkin Elmer PinAAcle 900T, Waltham, MA, USA).

Atomic absorption spectroscopy (AAS) was used to determine the intracellular accumulation after treatment with the platinum compounds. ciPTECs were seeded in T75 flasks and cultivated as stated before. Matured cells were treated for 2 h with 50 µM of cisplatin, oxaliplatin or PN149 or 300 µM carboplatin. Cells were then harvested via centrifugation and total cell count was determined. Afterwards, cell pellets underwent an acidic digestion using a solution of 30% H₂O₂ and 65% HNO₃ (1:1 (v:v)) (Roth, Karlsruhe, Germany) and evaporation. The residue was dissolved in 0.2% HNO₃ and platinum amount was analyzed at a wavelength of 26,594 nm in a graphite furnace using a PinAAcle 900 T (Perkin Elmer, Waltham, MA, USA). Atomization was performed using a furnace temperature program consisting of two drying steps of 120 °C for 30 s and 140 °C for 45 s followed by a pyrolysis step of 1300 °C for 20 s, an atomization step of 2400 °C for 5 s as well as a heating step for clean out of 2500 °C for 5 s. Intracellular accumulation was determined in three independent experiments and was calculated as ng Pt/10⁶ cells.

The alkali-hydrolyzed nitrogen (Avail.N.), available phosphorus (Avail.P.), and available potassium (Avail.K.) in biogas slurry were determined according to the Agricultural Standard of the People's Republic of China (27 –29 ). pH (30 ) was measured by a Seven Compact pH meter (Mettler Toledo, Switzerland). Organic matter (OM) was measured by the potassium dichromate method (31 ). TN (32 ) in soils and protein (33 ) in peanut kernels was determined by an automatic Kjeldahl nitrogen determination apparatus (KD-310, Opsis, Sweden), whereas the hydrolysis diffusion method was used to determine Avail.N. Total phosphorus (TP) and Avail.P. were determined by a spectrophotometer (UV-2600, Shimadzu, Japan). The soil, biogas slurry, and peanut samples were digested by acids (HNO₃-HClO₄-HF). The contents of Cu, Zn, Pb, Cr, total potassium (TK), Avail.K., and Cd in the samples were determined by atomic absorption spectrophotometry (PinAAcle 900T, PerkinElmer, Waltham, MA, USA). The contents of As and Hg were determined by an atomic fluorescence spectrophotometer (AFS-922, Titan Instruments, Beijing). The main stem height and lateral branch length of peanut plants were directly measured, and the branch number and fruit number per plant were directly counted.

In this study, atomic absorption spectrometer PinAAcle 900T (Perkin Elmer, Norwalk, CT, USA) has been used for determination of metal ion concentrations using air-acetylene flame mode. Concentrations of samples were detected after calibration with the spectrometer, with standards specific for each of the metal ions in the concentration range of 0.1–0.5 mg/L.
Thermal analysis of solid MS mass before and after contact with simulated textile effluent were done on a STA 409 PC Luxx simultaneous thermogravimeter-differential scanning calorimeter TG/DSC (Netzsch, Selb, Wunsiedel, Germany). Additionally, solid phases of MS were quantified using scanning electron microscope (SEM) Quanta FEG 250 Fei, Eindhoven, The Netherlands.
The XT220A Precise Gravimetrics scale, Dietikon, Switzerland was employed to weigh the MS masses.
The pH of supernatant solutions before and after adsorption was monitored with the HI 255 pH meter, Hanna Instruments, Nijverheidslaan, Belgium.
Ultrapure water of 18 MΩ/cm was obtained, with an Ultra-Clear system, Bremen, Germany.

The accumulation of Cd in rice “P” and “N” tissues and grain were determined by atomic absorption spectrophotometer (PerkinElmer PinAAcle 900 T, USA). Tissues were drying in a drying box (105 °C for 30 min and at 80°Cto constant weight). 50 mg powder samples were immersed into 1 mL HNO₃ and digested to transparent solutions. Cooled samples were then diluted into water to a final volume of 13.5 mL. Standard Cd solution was used as quality control samples. Besides, the expression of OsMAPK, OsHMA3, OsZIP4 and OsPCS were detected by qRT-PCR method.

Soil pH was determined using a glass electrode in a soil-water solution (w/v). The moisture content was determined by oven-drying for 48 h at 105°C. The soil organic matter (SOM) content was determined using the potassium dichromate oxidation method (Yu et al., 2020 (link)). Soil total nitrogen (TN) and total carbon (TC) content were determined using an automatic elemental analyzer (PerkinElmer 2400 Series II, United States; Tan et al., 2022 (link)). Soil nitrate-nitrogen (NO₃⁻-N) and ammonium-nitrogen (NH₄⁺-N) were extracted with 2 M KCl and determined using an UV spectrophotometer (Shimadzu UVmini-1285, Japan; Huang et al., 2019 (link)). Total phosphorus (TP) and total potassium (TK) were digested with HNO₃-HF-HClO₄, and the available phosphorus (AP) and available potassium (AK) were extracted with HCl-H₂SO₄ and ammonium acetate, respectively. TP and AP were determined using an atomic absorption spectrometer (PerkinElmer PinAAcle 900T, United States), and TK and AK were determined using an ultraviolet spectrophotometer, respectively (Jiang et al., 2017 (link)).