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Perma hand silk suture

Manufactured by Johnson & Johnson
Sourced in Canada, Germany

Perma-hand silk suture is a sterile, non-absorbable surgical suture made from high-quality silk fibers. It is designed for use in general soft tissue approximation and/or ligation.

Automatically generated - may contain errors

2 protocols using perma hand silk suture

1

Adipocyte AMPK Deletion and FGF21 Effects

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All experiments were approved by the McMaster University Animals Ethics Committee and conducted under appropriate Canadian guidelines for animal research. Mice lacking adipocyte AMPK β1β2 (iβ1β2AKO) along with littermate controls were generated as previously described [23] (link). Tamoxifen (Cayman Chemical; MI, USA), dissolved in sunflower oil, was given via oral gavage for five consecutive days in adult males to induce the deletion of adipocyte AMPK β1β2 after 10 weeks of a high fat diet (HFD, 45% kcal fat) (D12451, Research Diets; New Brunswick, New Jersey). At week 14 of HFD, control and iβ1β2AKO mice were anesthetized with Avertin (2,2,2-tribromoethanol dissolved in methyl-2-butanol, Sigma-Aldrich; ON, Canada; 0.5 mg/g body) and implanted dorsally with two week mini-osmotic pumps (1002, Alzet; CA, USA) delivering 0.35 mg/kg/day of native FGF21 or 0.9% sodium chloride (saline) and incisions closed with perma-hand silk suture (Ethicon; ON, Canada). Food and water were provided ad libitum and mice were housed at 23 °C with a 12-hour light/dark cycle.
Male ACC1-S79A and ACC2-S212A knock-in mutation mice (ACC DKI) were as previously described and were put on a 45% HFD at 3–4 months of age [31] . At week 10 of HFD, wildtype and ACC DKI mice received subcutaneous injections of long-acting FGF21 (PF-05231023) at 10 mg/kg twice a week for 2 weeks.
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2

Viral Injections in Mouse Hippocampus

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Viral injections were performed under aseptic conditions in Prox1::cre or C57BL/6 mice up to 10 months old. The mice were anesthetized with a mixture of Fentanyl (Rotexmedica, Germany), Midazolam (Rotexmedica, Germany), and Domitor (Orion Pharma, Finland) via intraperitoneal injection ( 0.05/5.0/0.5  mg/kg ). Analgesia ( 0.05  mg/kg of buprenorphine; Buprenovet, Bayer, Germany) was administered intraperitoneally prior to the injection, and Xylocain (AstraZeneca, Germany) was used for local anesthesia. Stereotaxic injections were performed using an injection frame (WPI Benchmark/Kopf) and a microprocessor-controlled minipump (World Precision Instruments, Sarasota, Florida), 1000 nl of the viral solution was injected bilaterally into the hippocampus (rAAV-DIO-eGFP; rAAV-syn-GFP; coordinates: rostrocaudal: 2.1  mm from the Bregma; mediolateral: ±1.2  mm from the midline; dorsoventral: 2.1  mm ). After the injection, the scalp was sutured with PERMA-HAND Silk Suture (Ethicon), and an antibacterial ointment (Refobacin®, Almirall, Germany) was applied, followed by the intraperitoneal injection of a mixture of Naloxon (B. Braun, Germany), Flumazenil (B. Braun, Germany), and Antisedan (Orion Pharma, Finland) ( 1.2/0.5/2.5  mg/kg ). To prevent the wound pain, analgesia was administered on the 3 following days.
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