Balb cbyj balb c

NOD.CB17-Prkdcscid/J (NOD SCID), CBA/J, BALB/cByJ (BALB/c) and transgenic C57/B6-GFP [C57BL/6-Tg (UBC-GFP) 30Scha/J] mice were obtained from the Jackson Laboratory, USA. Animals were maintained as per ethical guidelines (CPCSEA). Breeding strategy to obtain double homozygous mutant phenotype is described in detail in supplementary Materials and Methods.

Seven- to 8-week-old WT C57BL/6J (C57BL/6 or WT, The Jackson Laboratory, stock no. 00064), B6(c)-H2-Ab1^bm12/KhEgJ (BM12, The Jackson Laboratory, stock no. 001162), B6.129S2-Cd28^tm1Mak/J (CD28KO, The Jackson Laboratory, stock no. 002666), BALB/cByJ (BALB/c, The Jackson Laboratory, stock no. 001026), B6.129S7-Rag1^tm1Mom/J (RAGKO, The Jackson Laboratory, stock no. 002216), B6N.Cg-Tg(ACTFLPe)9205Dym/CjDswJ (FLPe B6N, The Jackson Laboratory, stock no. 019100), and B6.Cg-Tg(Cd4-Cre)1Cwi/BfluJ (CD4-Cre, The Jackson Laboratory, stock no. 022071) mice were used. PI3Kγ^−/− C57BL/6 mice (PI3KγKO, backcrossed 11 generations) were received from Bao Lu (Boston Children’s Hospital/Harvard Medical School, Boston, Massachusetts, USA) and maintained in our animal facility (126 (link)).
Pik3cg^{tm1a(EUCOMM)Wtsi} (Pik3cg-tm1a) mice were purchased from Wellcome Trust Sanger Institute (Hinxton, United Kingdom). Male and female mice were housed in sterilized and ventilated cages in a specific pathogen–free animal facility under a standard 12-hour light/12-hour dark cycle. Mice were fed irradiated food and water ad libitum. All experiments were performed with age- and sex-matched, 8- to 12-week-old mice in accordance with the relevant guidelines and regulations approved by the IACUC of Brigham and Women’s Hospital.

All the mice required during different experiments (NOD.CB17-Prkdcscid/J (NOD SCID), CBA/J and BALB/cByJ (BALB/c) were procured from the Jackson Laboratory, USA while the NOD-SCID-rd1 mouse model was developed in-house as reported previously [20 (link)]. The animals received ad libitum access to acidified autoclaved water and food. The temperature and humidity of the housing room was maintained at 21–23 °C and 40–60% respectively. Animals were kept at a 14-h light to 10-h dark cycle. Animals were randomly assigned in each group, and experiments were repeated at least twice. All animal experiments and reporting adhere to the ARRIVE guidelines [21 (link)].