The extracts of primary cultures taken at 36 h or 8 days after plating were subjected to standard procedures. The protein amount was determined using BioRad protein assay. Equal amounts of protein (30 µg) were electrophoresed on 10% acrylamide gel and electrotransferred to polyvinylidene fluoride membrane, followed by immunoblotting with antibodies for myosin heavy chain (MyHC; 1:1000, GTX73432, GeneTex, Taiwan), phospho-TAK1 (1:1000, Cell signaling, #4508), p-p38 (1:1000, Cell signaling, #4511), p-JNK (1:1000, GTX52328, GeneTex, Taiwan), p-ERK (1:1000, Cell signaling, #4370), and p-NF-kB (1:1000, Cell signaling, #3033). Goat anti-actin-HRP (1:5000, Santa Cruz Biotechnology) was used for protein quantification. The membrane was washed and incubated with secondary anti-mouse IgG-HRP (1:3000, sc-2005, Santa Cruz, California, USA), anti-goat IgG-HRP (1:3000, sc-2020, Santa Cruz, California, USA), and anti-rabbit IgG-HRP (1:3000, GTX213110-01, GeneTex, Taiwan). Detection was performed using the T-Pro LumiLong Plus Chemiluminescence Detection Kit (JT96-K004M, T-Pro Biotechnology, Taiwan) and the blots were directly processed on an Amersham Imager 600 (GE, Boston, USA). Densitometry was performed using ImageJ.
Gtx73432
Manufactured by GeneTex
GTX73432 is a laboratory equipment product. It is designed for general laboratory use. The core function of this product is to facilitate various experimental tasks and procedures in a research setting.
Automatically generated - may contain errors
Lab products found in correlation
Protein assay, by Bio-Rad (3 mentions)
Gtx52328, by GeneTex (3 mentions)
Goat anti actin hrp, by Santa Cruz Biotechnology (3 mentions)
Anti mouse igg hrp, by Santa Cruz Biotechnology (3 mentions)
Anti goat igg hrp, by Santa Cruz Biotechnology (3 mentions)
Anti rabbit igg hrp, by GeneTex (3 mentions)
Amersham imager 600, by GE Healthcare (3 mentions)
P erk, by Cell Signaling Technology (2 mentions)
3 protocols using gtx73432
1
Protein Analysis of Myogenic Markers
2
Protein Expression Analysis in Primary Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extracts of primary cultures taken at 36 h or 8 days after plating were subjected to standard procedures. The protein amount was determined using BioRad protein assay. Equal amounts of protein (30 µg) were electrophoresed on 10% acrylamide gel and electrotransferred to polyvinylidene uoride membrane, followed by immunoblotting with antibodies for myosin heavy chain (MyHC; 1:1000, GTX73432, GeneTex, Taiwan), phospho-TAK1 (1:1000, Cell signaling, #4508), p-p38 (1:1000, Cell signaling, #4511), p-JNK (1:1000, GTX52328, GeneTex, Taiwan), p-ERK (1:1000, Cell signaling, #4370), and p-NF-kB (1:1000, Cell signaling, #3033). Goat anti-actin-HRP (1:5000, Santa Cruz Biotechnology) was used for protein quanti cation. The membrane was washed and incubated with secondary anti-mouse IgG-HRP (1:3000, sc-2005, Santa Cruz, California, USA), anti-goat IgG-HRP (1:3000, sc-2020, Santa Cruz, California, USA), and anti-rabbit IgG-HRP (1:3000, GTX213110-01, GeneTex, Taiwan). Detection was performed using the T-Pro LumiLong Plus Chemiluminescence Detection Kit (JT96-K004M, T-Pro Biotechnology, Taiwan) and the blots were directly processed on an Amersham Imager 600 (GE, Boston, USA). Densitometry was performed using ImageJ.
3
Protein Expression Analysis in Primary Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extracts of primary cultures taken at 36 h or 8 days after plating were subjected to standard procedures. The protein amount was determined using BioRad protein assay. Equal amounts of protein (30 µg) were electrophoresed on 10% acrylamide gel and electrotransferred to polyvinylidene uoride membrane, followed by immunoblotting with antibodies for myosin heavy chain (MyHC; 1:1000, GTX73432, GeneTex, Taiwan), phospho-TAK1 (1:1000, Cell signaling, #4508), p-p38 (1:1000, Cell signaling, #4511), p-JNK (1:1000, GTX52328, GeneTex, Taiwan), p-ERK (1:1000, Cell signaling, #4370), and p-NF-kB (1:1000, Cell signaling, #3033). Goat anti-actin-HRP (1:5000, Santa Cruz Biotechnology) was used for protein quanti cation. The membrane was washed and incubated with secondary anti-mouse IgG-HRP (1:3000, sc-2005, Santa Cruz, California, USA), anti-goat IgG-HRP (1:3000, sc-2020, Santa Cruz, California, USA), and anti-rabbit IgG-HRP (1:3000, GTX213110-01, GeneTex, Taiwan). Detection was performed using the T-Pro LumiLong Plus Chemiluminescence Detection Kit (JT96-K004M, T-Pro Biotechnology, Taiwan) and the blots were directly processed on an Amersham Imager 600 (GE, Boston, USA). Densitometry was performed using ImageJ.
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