2′-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic acid (MUNANA, Sigma, M8639) is a fluorescent substrate for the neuraminidase (NA) of influenza viruses and is used to detect the replication levels of influenza viruses; therefore, it can also be used to measure the inhibitory effect of agents against influenza virus infection (An et al.2014 (link)). Briefly, the virus-containing culture supernatant was transferred to a black opaque 96 or 384 well plate (PerkinElmer, 6005270 or 6007270) and mixed with 20 μmol/L of MUNANA dissolved in MES solution (33 mmol/L 2-[N-morpholino] ethanesulfonic acid and 4 mmol/L CaCl2, pH = 6.5), followed by incubation at 37 °C for 1 h. The reaction was terminated by the addition of stop solution (0.14 mol/L NaOH in 83% ethanol). Fluorescence intensity was measured at an excitation wavelength of 355 nm and an emission wavelength of 485 nm using multi-label plate readers (Envision2103, PerkinElmer, USA).
Munana
Manufactured by PerkinElmer
MUNANA is a fluorogenic substrate used in fluorometric assays for the detection and quantification of enzymatic activity. It is commonly used in the analysis of neuraminidase (sialidase) enzymes. The core function of MUNANA is to serve as a reagent for fluorescence-based enzymatic activity measurements.
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2 protocols using munana
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Influenza virus neuraminidase activity assay
2
Neuraminidase Activity Assay for Influenza Viruses
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The fluorescent substrate, 2′-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (MUNANA, Sigma, M8639), for the neuraminidase (NA) of influenza viruses was used to detect the levels of NA. Briefly, the virus-containing culture supernatant was transferred to a black opaque 96 or 384 well plate (PerkinElmer, 6005270 or 6007270) and mixed with 20 µmol/L of MUNANA dissolved in MES solution (33 mmol/L 2-[N-morpholino] ethanesulfonic acid and 4 mmol/L CaCl2, pH = 6.5), followed by incubation at 37 °C for 1 h. The reaction was terminated by the addition of stop solution (0.14 mol/L NaOH in 83% ethanol). Fluorescence intensity was measured at an excitation wavelength of 355 nm and an emission wavelength of 485 nm using multi-label plate readers (Envision2103, PerkinElmer, Waltham, MA, USA).
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