Anti phospho c fos

Manufactured by Cell Signaling Technology

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Anti-phospho-c-Fos is a laboratory reagent used to detect the phosphorylation of the c-Fos protein. c-Fos is a transcription factor that plays a role in cellular processes such as proliferation and differentiation. The antibody can be used in various immunoassays to measure the levels of phosphorylated c-Fos in biological samples.

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C-Fos (167 citations) Anti-c-Fos (37 citations) Phospho-c-Fos (16 citations) Anti-FOS (7 citations) Anti-phospho-c-Fos (Ser32) (3 citations)

8 protocols using anti phospho c fos

Western Blot Analysis of Phosphorylated Proteins

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OKF6/TERT-2 cells in 24-well tissue culture plates were infected with 1
× 10⁶C. albicans for various times as described previously
¹⁶. Next, the cells
were rinsed with cold HBSS containing protease and phosphatase inhibitors and
removed from the plate with a cell scraper. After collected the cells by
centrifugation, they were boiled in sample buffer. The lysates were separated by
SDS/PAGE, and the phosphorylated proteins were detected by immunoblotting with
phospho-specific antibodies, including anti-phospho-EphA2 (Cell signaling;
#6347), anti-phospho-Stat3 (Cell signaling; #9134),
anti-phospho-c-Fos (Cell signaling; #5348), anti-phospho-MEK1/2 (Cell
signaling; #9154), anti-phospho-p65 (Cell signaling; # 3033).
The blots were then stripped, and total protein levels and β-actin were
detected by immunoblotting with appropriate antibodies against EphA2 (Cell
signaling; #6997), EphB2 (Cell signaling; #83029), EphA4 (Santa
Cruz; sc-365503), Stat3 (Cell signaling; # 12640), c-Fos (Cell
signaling; # 4384), MEK1/2 (Cell signaling; # 9122), p65 (Cell
signaling; # 8242), and β-actin (Cell signaling # 3700).
The blots were developed using enhanced chemiluminescence and imaged with either
a FluorChem 8900 (Alpha Innotech) or C400 (Azure biosystems) digital imager.

Swidergall M., Solis N.V., Lionakis M.S, & Filler S.G. (2017). EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans. Nature microbiology, 3(1), 53-61.

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Western Blot Analysis of Signaling Pathways

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Protein extraction solution PRO-PREP^TM was obtained from iNtRON Biotechnology (Jungwon, Gyeonggi, Korea). Polyvinylidene fluoride membranes and western chemiluminescent HRPV substrate were obtained from Millipore Corporation (Billerica, MA, USA). The above reagents were used according to the manufacturer’s protocol. The following antibodies from Cell Signaling Technology (Danvers, MA, USA) were used: EGFR antibody, anti-phospho-Src family (Tyr416), anti-phospho-AKT (Ser473), anti-phospho-Erk1/2, anti-p47^phox, anti-phospho-NF-κB p65, anti-phospho-c-Fos, anti-phospho-c-Jun, anti-p44/42 MAPK (ERK1/2), anti-NF-κB p65, and anti-β-actin monoclonal antibodies. The detailed western blot steps were described previously [13 (link)].

Li S., Nguyen T.T., Ung T.T., Sah D.K., Park S.Y., Lakshmanan V.K, & Jung Y.D. (2022). Piperine Attenuates Lithocholic Acid-Stimulated Interleukin-8 by Suppressing Src/EGFR and Reactive Oxygen Species in Human Colorectal Cancer Cells. Antioxidants, 11(3), 530.

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Immunohistochemical Analysis of Neuronal Activation

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Mice were deeply anesthetized with a 2/1 combination of ketamine (body weight, Vedco, Missouri, USA) and xylaxine (Akorn, Illinois, USA), perfused with phosphate-buffered saline followed by 3.7% formaldehyde (Fisher Scientific, NH, USA), and brains were post-fixed for 24 h. Brains were sliced in ¼ series of 50 μm sections using a cryostat, allowing the use of alternate slices for multiple IHC experiments. Free-floating sections were rinsed in PBS-Triton (0.25%) and incubated in normal goat serum and primary antibodies (Anti-phospho-cFos, Cell Signaling Technology, #5348, 1:1000, MA, USA; Anti-NeuN, #MAB377, 1:1000, Millipore Sigma, MA, USA) overnight at 4 °C. After multiple PBS-Triton rinses, sections were incubated in Alexa-Fluor conjugated secondary antibodies (Life Technologies, 1:1000).

Bobadilla A.C., Dereschewitz E., Vaccaro L., Heinsbroek J.A., Scofield M.D, & Kalivas P.W. (2020). Cocaine and sucrose rewards recruit different seeking ensembles in the nucleus accumbens core. Molecular psychiatry, 25(12), 3150-3163.

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Negative Ion Generator Effects on Immune Signaling

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The negative ion generator Dermio Care® (Weyergans High Care AG, Western Rhineland, Germany) generating 4×10⁵ negative ions/cm³ was used in this study. The standard reference materials (SRMs) 1648a (pmA) and 1649b (pmB) were acquired from the National Institute of Standards and Technology (NIST; Gaithersburg, MD, USA) and dispersed in distilled water. Specific antibodies used for Western blot analysis, including anti-phospho-p38 MAPK, anti-p38 MAPK, anti-phospho-JNK MAPK, anti-JNK MAPK, anti-phospho-ERK1/2 MAPK, anti-ERK1/2 MAPK, anti-phospho-c-Fos, anti-phospho-c-Jun, anti-phospho-p65, and anti-p65 antibodies, were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Kim M., Jeong G.J., Hong J.Y., Park K.Y., Lee M.K, & Seo S.J. (2021). Negative Air Ions Alleviate Particulate Matter-Induced Inflammation and Oxidative Stress in the Human Keratinocyte Cell Line HaCaT. Annals of Dermatology, 33(2), 116-121.

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Comprehensive Protein Expression Analysis

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Primary antibodies used for Western blot analysis and immunohistochemistry: mouse monoclonal anti-Ki67 (clone MIB-1) (Dako, Glostrup, Denmark), mouse monoclonal anti-cyclin A and anti-cyclin E (Invitrogen, Carlsbad, CA), mouse monoclonal anti-p27 and anti-p21 (Santa Cruz Biotechnology, Heidelberg, Germany), mouse monoclonal anti-tubulin (Sigma-Aldrich, St Louis, MO), rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2), anti-p44/42 MAPK (Erk1/2), anti-phospho-Akt, anti-Akt, anti-phospho-c-Jun, anti-c-Jun, anti-phospho-c-Fos, anti-c-Fos, anti-phospho-CREB, anti-CREB (Cell Signaling Technology, Danvers, MA). Secondary antibodies coupled to horseradish peroxidase were obtained from Amersham Pharmacia Biotech (Diegem, Belgium). Palbociclib (PD-0332991) was purchased at Selleck Chemicals (Munich, Germany).

Lore L., An H., Evelyne L., Mieke V.B., Jo V., Dawn M., Geert B., Rudy V.D., Cathérine M., Marc B., Véronique C., Hannelore D, & Olivier D.W. (2017). Secretome analysis of breast cancer-associated adipose tissue to identify paracrine regulators of breast cancer growth. Oncotarget, 8(29), 47239-47249.

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Inflammatory Signaling Pathway Analysis

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Ficoll-Paque was purchased from GE Healthcare (Little Halfont, Buckinghamshire, UK). Ultrapure LPS was purchased from InvivoGen (California, USA). Anti-IL-1β, anti-p65, anti-phospho-p65, anti-p38, anti-phospho-p38, anti-ERK, anti-phospho-ERK, anti-JNK, anti-phospho-JNK, anti-c-Jun, anti-phospho-c-Jun, anti-phospho-c-Fos and anti-c-Fos were all purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-G6PD was purchased from Genesis Biotech (Taiwan). ATP, nigericin, PMA, hydrogen peroxide, DPI, NADPH, and anti-β-Actin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-NLRP3 was purchased from AdipoGen Life Sciences (San Diego, CA, USA), and anti-ASC was purchased from Medical & Biological Laboratories (Japan). The anti-caspase-1, anti-pro-IL-1β, anti-mouse and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-α-tubulin was purchased from Merck Millipore (Burlington, USA). Anti-lamin B1 was purchased from Proteintech (Illinois, USA). Polyvinylidene difluoride (PVDF) membranes and Immobilon Western Chemiluminescent HRP Substrate (ECL) were purchased from Millipore Corporation (Billerica, MA, USA).

Yen W.C., Wu Y.H., Wu C.C., Lin H.R., Stern A., Chen S.H., Shu J.C, & Tsun-Yee Chiu D. (2019). Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling. Redox Biology, 28, 101363.

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Gallic Acid-Mediated Gold Nanoparticle Synthesis

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Gallic acid, hydrogen tetrachloroaurate (HAuCl₄), and collagen I were obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA). The CellTiter 96^® aqueous one-solution proliferation assay kit was obtained from Promega Corporation Inc. (Madison, WI, USA). H₂DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Anti-MMP-1 and anti-MMP-3 were purchased from Abcam (San Francisco, CA, USA). Anti-β-actin and peroxidase conjugated secondary antibodies were obtained from EMD Millipore Inc. (Burlington, MA, USA). Anti-phospho-NFκB p65 (Ser536), anti-NFκB p65, anti-phospho-ERK, anti-ERK, anti-phospho-JNK, anti-JNK, anti-phospho-p38 MAPK, anti-p38 MAPK, anti-phospho-ASK1, anti-ASK1, anti-phospho-c-Jun, anti-c-Jun, anti-phospho-c-Fos, anti-c-Fos, anti-phospho-ATF-2, and anti-ATF-2 antibodies were the products of Cell Signaling Technology (Beverly, MA, USA). Cell culture supplies were purchased from GIBCO/Life Technologies Inc. (Carlsbad, CA, USA).

Wu Y.Z., Tsai Y.Y., Chang L.S, & Chen Y.J. (2021). Evaluation of Gallic Acid-Coated Gold Nanoparticles as an Anti-Aging Ingredient. Pharmaceuticals, 14(11), 1071.

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Western Blot Analysis of Phosphorylated Proteins

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Swidergall M., Solis N.V., Lionakis M.S, & Filler S.G. (2017). EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans. Nature microbiology, 3(1), 53-61.

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