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6 0 nylon monofilament

Manufactured by Johnson & Johnson
Sourced in United States

6-0 nylon monofilament is a surgical suture material made of nylon. It is a single, continuous thread without any braiding or twisting. The '6-0' designation refers to the suture size, which indicates the diameter of the thread.

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7 protocols using 6 0 nylon monofilament

1

Wireless Optoelectronic Implants for Mice

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The devices consisted of wireless, battery-free miniaturized optoelectronic systems based on NFC technology (~ 10 mm × 8 mm × 1 mm - at the thickest regions). Mice were anesthetized with isoflurane (induction value: 3 %; maintenance value: 1.5 – 2 %), received ketoprofen (5 mg/kg, i.p.) for analgesia, and were placed on a small animal stereotaxic frame (David Kopf Instruments, CA, USA) for the surgery. Puralube vet ointment (Dechra Veterinary Products, KS, USA) was applied to cover the eyes during this procedure. A 1-cm-long incision was made over the shaved back to expose the subcutaneous tissues. The device was then placed on the subcutaneous tissue, followed by a PDMS mold to control the spread of the liquid precursor mixture. UV exposure followed the application of primers and precursors into this mold, and directly onto the device and adjacent regions of the tissue. After implantation, nylon 6-0 monofilament (Ethicon Inc., NJ, USA) sutures were applied to close the wounds, with standard post-surgical recovery procedures.
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2

Wireless Optoelectronic Implants for Mice

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The devices consisted of wireless, battery-free miniaturized optoelectronic systems based on NFC technology (~ 10 mm × 8 mm × 1 mm - at the thickest regions). Mice were anesthetized with isoflurane (induction value: 3 %; maintenance value: 1.5 – 2 %), received ketoprofen (5 mg/kg, i.p.) for analgesia, and were placed on a small animal stereotaxic frame (David Kopf Instruments, CA, USA) for the surgery. Puralube vet ointment (Dechra Veterinary Products, KS, USA) was applied to cover the eyes during this procedure. A 1-cm-long incision was made over the shaved back to expose the subcutaneous tissues. The device was then placed on the subcutaneous tissue, followed by a PDMS mold to control the spread of the liquid precursor mixture. UV exposure followed the application of primers and precursors into this mold, and directly onto the device and adjacent regions of the tissue. After implantation, nylon 6-0 monofilament (Ethicon Inc., NJ, USA) sutures were applied to close the wounds, with standard post-surgical recovery procedures.
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3

Preconditioning Induces Focal Ischemia in Mice

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During the procedures to induce IPC and focal ischemia, mice were placed under anesthesia with ketamine (100 mg/kg) administered intraperitoneally. For IPC, both common carotid arteries were exposed and ligated with 6-0 silk sutures three times for 1 min each. Between each ligation, the arteries were reopened for 5 min [28] (link). Sham-operated mice underwent the same procedure without ligation. Permanent focal ischemia was produced by intraluminal middle cerebral artery (MCA) occlusion (MCAO) for 24 h with a 6-0 nylon monofilament (Ethicon, Somerville, NJ, USA). Successful occlusion of the MCA was verified and recorded by laser-Doppler flowmetry (Moor VMS-LDF, Devon, UK). Groups of 9 mice underwent MCAO 24 h after preconditioning or the sham operation.
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4

Transient Focal Ischemia in Mice

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Transient focal ischemia was induced in male and female mice for 60 min by reversible middle cerebral artery occlusion (MCAO) under isoflurane anesthesia followed by 96 h of reperfusion, as previously described [3 (link), 13 (link)], Briefly, mice were anesthetized with isoflurane, 5% induction and 2% maintenance, then the right common carotid artery (CCA) and external carotid artery (ECA) were exposed and the ECA was ligated. A 6–0 nylon monofilament (ETHICON, Inc., Somerville, NJ) was advanced into the ECA and up the internal carotid artery (ICA) to the base of the middle cerebral artery (MCA) occluding blood flow to the MCA territory. The filament was left in place for 60 min and then withdrawn to allow reperfusion of the MCA. After reperfusion the mice were survived for 96 h. To ensure adequate drops in blood flow during occlusion and increases in flow during reperfusion, cerebral blood flow was monitored by Laser Doppler Flowmetry (LDF) (Model DRT4, Moor Instruments Ltd., Wilmington, DE) with a probe fixed to the skull during the surgery. Mice were excluded from the study if the mean intra-ischemic LDF was greater than 30% of the pre-ischemic baseline LDF. No mice were excluded from the study. During the surgery mouse body and head temperature was maintained at 37±1.0°C with a warm water blanket and a heating lamp.
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5

Wireless Optogenetic Neural Control

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The devices consisted of wireless optogenetic platforms based on NFC technology for untethered neuronal control and discrete spatiotemporal targeting of neural circuits. The constituent components included a penetrating probe with a microscale inorganic light-emitting diode (μ-ILED) at its tip end for insertion into the deep brain and a receiver coil for wireless power harvesting above the skull in the subcutaneous tissues. The fabrication process followed procedures described previously21 (link)43 (link),45 (link). Standard anesthesia and eye protection to mice proceeded as described above. A 5-mm-long incision made on the scalp exposed the skull allowed for drilling a hole for inserting the probe into the targeted brain regions. Coordinates for medial prefrontal cortex (mPFC) probe placement were + 2.0 mm (AP), + 0.4 mm (ML), and +1.5 mm (DV). The BTIM encapsulated the receiver coil and anchored the platform onto the skull. A 6-0 nylon monofilament (Ethicon Inc., NJ, USA) suture closed the wound after implantation.
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6

Surgical Injury to Trigeminal Nerve

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Mice were anesthetized with isoflurane inhalation. Surgery was performed under an Omano surgical microscope (OM2300S-V7, 7-40X). A 1–1.5 cm midline neck incision was used. The superficial tissues were bluntly dissected and lateralized with a mini retractor. The neck muscles were gently dissected to locate the mouse’s right auditory bulla and the auditory capsule on the right side of the head, which are the landmarks to locate the foramen lacerum. A prepared piece of Surgifoam (Ethicon) at approximately 1–1.5 mg was gently delivered into the foramen lacerum using curved forceps. The Surgifoam was positioned between the trigeminal nerve root and the cochlea bulla. After removing the retractor and replacing the tissues, the skin was closed with 6-0 nylon monofilament (Ethicon) sutures. Mice in the sham group underwent the same surgical procedure including neck shaving, skin incision, muscle dissection, and foramen lacerum exposure without nerve root compression. The duration of the surgery ranged from 8 to 12 minutes per mouse. Tamoxifen was intraperitoneally administrated to Fos-iCre-ERT2 (Fos2A-iCreER-knockin) mice immediately after the FLIT procedure.
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7

Wireless Optogenetic Neural Control

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The devices consisted of wireless optogenetic platforms based on NFC technology for untethered neuronal control and discrete spatiotemporal targeting of neural circuits. The constituent components included a penetrating probe with a microscale inorganic light-emitting diode (μ-ILED) at its tip end for insertion into the deep brain and a receiver coil for wireless power harvesting above the skull in the subcutaneous tissues. The fabrication process followed procedures described previously21 (link)43 (link),45 (link). Standard anesthesia and eye protection to mice proceeded as described above. A 5-mm-long incision made on the scalp exposed the skull allowed for drilling a hole for inserting the probe into the targeted brain regions. Coordinates for medial prefrontal cortex (mPFC) probe placement were + 2.0 mm (AP), + 0.4 mm (ML), and +1.5 mm (DV). The BTIM encapsulated the receiver coil and anchored the platform onto the skull. A 6-0 nylon monofilament (Ethicon Inc., NJ, USA) suture closed the wound after implantation.
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