7-week old male ICR mice used for the in vivo erythrocyte micronucleus test were procured from BioLASCO Taiwan Co., Ltd and were utilized after a week of acclimatization and quarantine. ICR mice were housed in groups of five in polypropylene cages with absorbent hardwood bedding (Beta Chip; Northeastern Products Corp, USA). The animals were maintained in a well-ventilated room (10–15 air changes/h) under an ambient temperature of 22 ± 3 °C and 55 ± 15% relative humidity on a 12:12 h light regime, and they were provided with standard rodent diet (MF-18; Oriental Yeast Co., Ltd, Tokyo, Japan) as well as purified water ad libitum. All husbandry conditions were conformed to the guidelines set forth by the National Institutes of Health (NIH) for the care and use of laboratory animals, and were carried out under Good Laboratory Practice (21 CFR Part 58). These protocols were approved by the Institutional Animal Care and Use Committee (IACUC 101-11e).
Mf 18
Manufactured by Oriental Yeast
Sourced in Japan
The MF-18 is a laboratory centrifuge designed for general-purpose applications. It is capable of processing samples at high speeds to separate components based on their density differences. The MF-18 features a compact and durable construction, ensuring reliable performance in various laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mf 18
1
ICR Mouse Erythrocyte Micronucleus Assay
2
Sprague-Dawley Rat Husbandry Protocol
3
HFSTZ Mice Model for Metabolic Disorders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HFSTZ Mice were created by feeding a high-fat diet and injecting single-dose STZ [3 (link),4 (link),5 (link)]. Briefly, 10 week-old mice were fed a HFD (60% energy from fat; TestDiet, St. Louis, MO, USA) with water ad libitum. For control, a normal chow diet (NCD; MF-18, Oriental Yeast Co. Ltd., Tokyo, Japan) was used. Mice on HFD also received intraperitoneal injections of STZ (50 mg/kg, in 0.1 M citrate buffer, pH 4.5) 2 weeks after HFD initiated (HFSTZ group). On the contrary, NCD mice were injected with vehicle (0.1 M citrate buffer, pH 4.5). The HFD continued for 11 weeks of HFD manipulation. To evaluate the effect of APS on ameliorating the impairment in HFSTZ mice, APS (500 mg/kg) was orally administrated twice per day for 7 weeks after 4 weeks of HFD administration. The body weight and blood glucose were recorded every week after dietary manipulations except the week for STZ injection.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!