Ag sybr green pro taqhs premix

The antibody GRK4 (Cat. No. 11808-1-AP) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-cleaved caspase−3 (Cat. No. 9661s) was purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-Caspase-3 (Cat. No. ab184787) was obtained from Abcam (Cambridge, MA, USA). Anti-β-Tubulin (Cat. No. T0023) was purchased from Affinity Biosciences (Cincinnati, OH, USA). The luciferase assay kit was obtained from BioVision (Milpitas, CA, USA). The lncRNA 148400 siRNA, miR−10b−3p mimic, miR−10b−3p inhibitor, GRK4 siRNA, and GRK4 plasmid were purchased from Ribo (Guangzhou, China). Lipofectamine 2000 was obtained from Life Technologies (Carlsbad, CA, USA). TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). The Ag SYBR Green Pro TaqHS premix was obtained from Accurate Biotechnology (China).

Anti-PAK7 and anti-β-tubulin antibodies were procured from Proteintech (USA). Anti-C3 (9662) and CC3 (9664) antibodies were supplied by Cell Signaling Technology (USA). Secondary antibodies were obtained from Affinity (USA). FITC-Annexin-V-Apoptosis-Detection-Kit-I (556,547; BD Pharmingen, USA); luciferase-assay-kit (BioVision, USA); AG-SYBR-Green-Pro-Taqhs-premix (Accurate Biotechnology, China); LPS (L2880; Sigma, USA).