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Ec plan neofluar 40x 1.3 oil

Manufactured by Zeiss
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The Zeiss EC Plan-Neofluar 40x/1.3 Oil is a high-performance microscope objective lens. It has a magnification of 40x and a numerical aperture of 1.3, which allows for high-resolution imaging. The lens is designed for use with oil immersion to enhance its optical performance.

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Lab products found in correlation

Mounting medium, by Agilent Technologies (2 mentions) H 140, by Santa Cruz Biotechnology (2 mentions) Sc 393325, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 or alexa fluor 594 labelled secondary antibody, by Thermo Fisher Scientific (2 mentions)

2 protocols using ec plan neofluar 40x 1.3 oil

1

Immunofluorescence Staining of Tfr2 and Osterix

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence staining, cells were grown on glass slides. At the desired time point, cells were fixed with 100% methanol for 15 min, permeabilized with 0.5% Triton X-100 for 10 min and after washing for three times, blocked with 1% BSA in PBS for 30 min. Afterwards, cells were incubated with an anti-mouse Tfr2 antibody (H-140, Santa Cruz) over night at 4 °C. After washing, cells were stained with an anti-mouse osterix antibody (sc-393325, Santa Cruz) or phalloidin at RT for 1 h. Subsequently, cells were washed and incubated for 1 h with an Alexa Fluor 488 or Alexa Fluor 594-labelled secondary antibody (Life Technologies), washed, and stained with DAPI for 5 min. After washing again, glass slides were embedded in a small droplet of mounting medium (Dako). Slides were examined using a Zeiss LSM 510 confocal microscope (Zeiss EC Plan-Neofluar 40x/1.3 Oil), and photographs were taken and processed with the Zen 2009 software.
Rauner M., Baschant U., Roetto A., Pellegrino R.M., Rother S., Salbach-Hirsch J., Weidner H., Hintze V., Campbell G., Petzold A., Lemaitre R., Henry I., Bellido T., Theurl I., Altamura S., Colucci S., Muckenthaler M.U., Schett G., Komla Ebri D., Bassett J.H., Williams G.R., Platzbecker U, & Hofbauer L.C. (2019). Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nature metabolism, 1(1), 111-124.
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2

Immunofluorescence Staining of Tfr2 and Osterix

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence staining, cells were grown on glass slides. At the desired time point, cells were fixed with 100% methanol for 15 min, permeabilized with 0.5% Triton X-100 for 10 min and after washing for three times, blocked with 1% BSA in PBS for 30 min. Afterwards, cells were incubated with an anti-mouse Tfr2 antibody (H-140, Santa Cruz) over night at 4 °C. After washing, cells were stained with an anti-mouse osterix antibody (sc-393325, Santa Cruz) or phalloidin at RT for 1 h. Subsequently, cells were washed and incubated for 1 h with an Alexa Fluor 488 or Alexa Fluor 594-labelled secondary antibody (Life Technologies), washed, and stained with DAPI for 5 min. After washing again, glass slides were embedded in a small droplet of mounting medium (Dako). Slides were examined using a Zeiss LSM 510 confocal microscope (Zeiss EC Plan-Neofluar 40x/1.3 Oil), and photographs were taken and processed with the Zen 2009 software.
Rauner M., Baschant U., Roetto A., Pellegrino R.M., Rother S., Salbach-Hirsch J., Weidner H., Hintze V., Campbell G., Petzold A., Lemaitre R., Henry I., Bellido T., Theurl I., Altamura S., Colucci S., Muckenthaler M.U., Schett G., Komla Ebri D., Bassett J.H., Williams G.R., Platzbecker U, & Hofbauer L.C. (2019). Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nature metabolism, 1(1), 111-124.
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