The lens opacity was first documented by a slit lamp (SL-D4; Topcon, Livermore, CA, USA) with 40× magnification every day for the first 12 days after UVB irradiation and then every 2 weeks. The mydriatic eye drop (0.5% tropicamide ophthalmic solution; NDC 1748-101-12; Akorn, Lake Forest, IL, USA) was given 5 minutes before the slit-lamp recording. Mice were anesthetized by isoflurane (46066-755-04; Aspen Veterinary Resources, Liberty, MO, USA) during the procedure. At the end of each experimental time point, the mice lenses were dissected and a darkfield image was taken with a Leica M80 microscope (Leica, Buffalo Grove, IL). Lastly, the lens capsule was dissected, and the capsular opacity was documented by a Leica M80 microscope and a Leica DMi1 phase-contrast microscope (Leica Microsystems, Wetzlar, Germany).
M80 microscope
Manufactured by Leica
Sourced in Germany
The Leica M80 microscope is a high-quality optical instrument designed for laboratory and research applications. It features a sturdy and ergonomic design, providing a stable platform for precise observations and measurements. The M80 offers a range of magnification options, allowing users to examine samples with clarity and detail. This microscope is a reliable and versatile tool for various scientific and technical disciplines.
Automatically generated - may contain errors
Lab products found in correlation
Ic80 hd camera, by Leica (4 mentions)
Sl d4, by Topcon (3 mentions)
Application suite v4, by Leica (2 mentions)
Sp8 laser scanning confocal microscope, by Leica (2 mentions)
Mc170 hd camera, by Leica (2 mentions)
Dmi1 phase contrast microscope, by Leica (2 mentions)
Na2hpo4, by Carl Roth (1 mentions)
Evans blue dye, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Nah2po4, by Merck Group (1 mentions)
Axio imager z2, by Zeiss (1 mentions)
Sp8 confocal microscope, by Leica (1 mentions)
Xylene, by Thermo Fisher Scientific (1 mentions)
Kl 1500 lcd, by Schott (1 mentions)
Histomount mounting medium, by National Diagnostics (1 mentions)
Axio zoom v16 microscope, by Zeiss (1 mentions)
Orca flash 4.0 c1140 22c camera, by Hamamatsu Photonics (1 mentions)
Tcs sp8 lightning, by Leica (1 mentions)
Las x software, by Leica (1 mentions)
Orbit 1000, by Labnet (1 mentions)
18 protocols using m80 microscope
1
Quantifying UVB-induced Lens Opacity
2
Quantitative Image Analysis Protocol
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Tracking images were acquired from videos using Imaris snapshot function. Brightfield images were acquired using a Leica M80 microscope equipped with a Leica MC170 HD camera. Images were enhanced in brightness, contrast and saturation using GIMP to improve the visual quality. Quantifications were not affected by imaging quality enhancing modifications.
Graphics and drawings were realized using Paint, GIMP and Power point.
Graphics and drawings were realized using Paint, GIMP and Power point.
3
Quantifying Reperfused Acute Myocardial Infarction
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TTC and Evan’s blue staining were used to quantify the size of reperfused acute myocardial infarction (repAMI) as described previously [23] , [26] (link). After removal, hearts were flushed blood-free with PBS (Sigma-Aldrich, St. Louis, MO, USA) via cannulation of the ascending aorta. Then LCA was re-occluded by suture followed by injection of 1 mL 1 % Evan’s blue dye (Sigma-Aldrich, St. Louis, MO, USA) to visualize the area at risk (AAR). Thereafter, the heart was seperated into 1–2 mm transversal cross-sections and incubated in 1 % TTC in 0.0774 M Na2HPO4 (Carl Roth, Karlsruhe, Germany) and 0.0226 M NaH2PO4 (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 5 min. Images were obtained with a M80 microscope and IC80 HD camera (Leica, Wetzlar, Germany) following analysis by a blinded investigator using ImageJ 1.53 (NIH, New York City, NY, USA) [27] (link).
4
Synchronized Worm Defecation Cycles
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Wild-type and mutant animals were synchronized by placing 20 gravid adult worms on NGM plates seeded with E. coli OP50 and allowing them to lay eggs for 45 min at 25°C. The gravid adult worms were then removed, and the eggs were allowed to hatch and grow at 20°C until they reached the young adult stage. The synchronized worms were transferred to NGM plates fully seeded with P. aeruginosa for 24 hours at 25°C. Worms were observed under a Leica M80 microscope with a 60× magnification at room temperature. For each worm, an average of 10 intervals between two defecation cycles were measured. The defecation cycle was identified as a peristaltic contraction beginning at the posterior body of the animal and propagating anteriorly followed by feces expulsion.
5
Multispecies Neuroscience Imaging
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C. elegans and mouse spinal cord imaging were performed
on a Leica SP8 laser scanning confocal microscope. Fly imaging was performed on
a Leica M80 microscope with the IC80 HD camera, and images were taken and
analyzed with Leica application suite V4 software.
on a Leica SP8 laser scanning confocal microscope. Fly imaging was performed on
a Leica M80 microscope with the IC80 HD camera, and images were taken and
analyzed with Leica application suite V4 software.
6
Planarian Regeneration Via RNAi
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Injection of double-stranded RNA (dsRNA) was performed as described previously (Sandmann et al., 2011 (link)). In double-knockdown experiments, 1.5 μg/ml of each dsRNA (3 μg/ml total) was injected. Control animals (ctrl) were injected with dsRNA against green fluorescent protein (gfp). dsRNAs were synthesized according to Boutros et al. (2004) (link). For regeneration experiments, planarians were either amputated pre- and post-pharyngeally or sagittally and observed at the time indicated. Live images were taken with a Leica M80 microscope. Primers for dsRNAs are listed in Table S2 .
7
Pharyngeal Pumping Rate Assay
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Wild-type and npr-8(ok1439) animals were synchronized by placing 20 gravid adult worms on NGM plates seeded with E. coli OP50 and allowing them to lay eggs for 45 min at 25°C. The gravid adult worms were then removed, and the eggs were allowed to hatch and grow at 20°C until they reached the young adult stage. The synchronized worms were transferred to NGM plates fully seeded with P. aeruginosa for 24 hours at 25°C. Worms were observed under the Leica M80 microscope with focus on the pharynx. The number of contractions of the pharyngeal bulb was counted over 30 s. Counting was conducted in triplicate and averaged to give a pumping rate.
8
Multispecies Neuroscience Imaging
9
Automated Worm Volumetric Measurement
10
Dose-Dependent Effects of E. coli on Worm Growth
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