Ti eclipse confocal microscopy system

Manufactured by Nikon

The Nikon Ti Eclipse confocal microscopy system is a versatile laboratory equipment designed for high-resolution imaging and analysis. It features a modular design that allows for the integration of various detection and illumination modules to meet the specific requirements of different research applications. The system utilizes confocal optics to provide optical sectioning and improved signal-to-noise ratio, enabling detailed visualization of complex samples.

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Lab products found in correlation

Glass coverslip dishes, by MatTek (3 mentions) Nis elements software, by Nikon (3 mentions)

Close spelling variants of this product

Eclipse Ti confocal microscopy Eclipse Ti microscopy system Eclipse Ti confocal system Eclipse Ti System Confocal microscopy system Eclipse Ti Confocal microscopy

3 protocols using ti eclipse confocal microscopy system

Calcium Signaling in RBL2H3 Cells

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RBL2H3 were plated on glass coverslip dishes (MatTek, Ashland, MA) and incubated with 1 µM Fluo-4 for 30 min at 37°C in modified Ringer’s solutions as described above. After washing, cells were stimulated as indicated on a 37°C heated stage. Calcium signals were acquired using a Nikon Ti Eclipse confocal microscopy system, using EZ C1 software for acquisition and NIS Elements software (Nikon) for analysis. Where indicated, nominally calcium-free external conditions were achieved by the preparation of 0 mM added CaCl₂ Ringer solution containing 1 mM EGTA.

Jansen C., Shimoda L.M., Starkus J., Lange I., Rysavy N., Maaetoft-Udsen K., Tobita C., Stokes A.J, & Turner H. (2019). In vitro exposure to Hymenoptera venom and constituents activates discrete ionotropic pathways in mast cells. Channels, 13(1), 264-286.

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Calcium Signaling in RBL2H3 Cells

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RBL2H3 were plated on glass coverslip dishes (MatTek, Ashland, MA) and incubated with 1μM Fluo-4 for 30 minutes at 37°C in a standard modified Ringer's solution as described above. After washing, cells were stimulated as indicated on a 37°C heated stage. Calcium signals were acquired using a Nikon Ti Eclipse confocal microscopy system, using EZ C1 software for acquisition and NIS Elements software (Nikon) for analysis. Where indicated, nominally calcium-free external conditions (indicated as ~0 mM) were achieved by the preparation of 0mM added CaCl2 Ringer solution containing 1mM EGTA.

Shimoda L.M., Showman A., Baker J.D., Lange I., Koomoa D.L., Stokes A.J., Borris R.P, & Turner H. (2015). Differential regulation of calcium signalling pathways by components of Piper methysticum (‘Awa). Phytotherapy research : PTR, 29(4), 582-590.

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RBL2H3 Calcium Signaling Assay

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RBL2H3 were plated on glass coverslip dishes (MatTek, Ashland, MA) and incubated with 1μM Fluo-4 for 30 minutes at 37°C in a standard modified Ringer's solution as described above. After washing, cells were stimulated as indicated on a 37° C heated stage. Calcium signals were acquired using a Nikon Ti Eclipse confocal microscopy system, using EZ C1 software for acquisition and NIS Elements software (Nikon) for analysis. Where indicated, nominally calcium-free external conditions were achieved by the preparation of 0mM added CaCl₂ Ringer solution containing 1mM EGTA.

Greineisen W.E., Speck M., Shimoda L.M., Sung C., Phan N., Maaetoft-Udsen K., Stokes A.J, & Turner H. (2014). Lipid body accumulation alters calcium signaling dynamics in immune cells. Cell calcium, 56(3), 169-180.

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