Anti‐HA (sc‐805), Myc (sc‐40), Peli3 (sc‐376466), IRF4 (sc‐130921), PCNA (sc‐56), His (H‐3; sc‐8036), Ub (sc‐8017) and β‐catenin (H‐102; sc‐7199) antibodies were obtained from Santa Cruz (Santa Cruz, CA, USA). Antibodies against total IκBα (sc847), phospho‐IκBα (9246), Erk1/2 (9102), phospho‐Erk1/2 (9101), STAT3 (9132), and phosphor‐STAT3 (9145) were purchased from Cell Signaling (Danvers, MA, USA). Anti‐β‐actin (AC‐15, A1978), Flag (F3165) and LPS were obtained from Sigma‐Aldrich (St. Louis, MO, USA).
β catenin h 102 sc 7199
Manufactured by Santa Cruz Biotechnology
Sourced in United States
β‐catenin (H‐102; sc‐7199) is an antibody produced by Santa Cruz Biotechnology. It is designed to detect β‐catenin, a key regulator of the Wnt signaling pathway, which plays a role in cell-cell adhesion and gene transcription.
Automatically generated - may contain errors
Lab products found in correlation
Phosphor stat3 9145, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
F actin, by Thermo Fisher Scientific (1 mentions)
Ob cadherin, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 labeled anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions)
α catenin, by Abcam (1 mentions)
Slowfade gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)
Ep1793y, by Abcam (1 mentions)
Bz 9000, by Keyence (1 mentions)
Pdvf membrane, by Merck Group (1 mentions)
Bax n 20 sc 493, by Santa Cruz Biotechnology (1 mentions)
Hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
V 630 spectrophotometer, by Jasco (1 mentions)
3 protocols using β catenin h 102 sc 7199
1
Antibody Characterization for Signaling Pathways
2
Immunofluorescence Analysis of Cadherin and Catenin Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells cultured on glass coverslips were rinsed several times in Ca2+- and Mg2+-containing PBS, fixed in 4% paraformaldehyde (PFA) for 10 min at 37°C, and permeabilized in 4% PFA containing 0.1% Triton X-100 for 1 min at room temperature. After blocking in 4% Block Ace solution (Yukijirushi Inc., Sapporo, Japan), the cells were incubated for 1 hr separately with primary antibodies: OB-cadherin (#4442; Cell Signaling Technology, Beverly, MA, USA) at 1:100 dilution; and EPLIN (16639-1-AP; Proteintech, Rosemont, IL, USA), β-catenin (H102 sc-7199; Santa Cruz, Dallas, TX, USA) and α-catenin (anti-α1 catenin antibody EP1793Y; Abcam, Cambridge, UK) all at a dilution of 1:50. After washing, bound antibodies were detected using Alexa Fluor 488-labeled anti-rabbit secondary antibody (Thermo Fisher Scientific) for 30 min. Then, F-actin (Phalloidin, Thermo Scientific) was stained in all slides. Cell nuclei were stained with DAPI (SlowFade Gold Antifade Mountant with DAPI; Thermo Fisher Scientific). As a negative control, normal rabbit IgG was used at the same concentration instead of the primary antibody in each experiment. Microphotos were taken using a fluorescence microscope (BZ-9000; Keyence, Osaka, Japan).
3
Investigating Molecular Markers in UVB-Treated Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After treating the HCT116 cells with UVB1, calcitriol or vehicle, they were lysed in lysis buffer containing 2% TRIS 1M, 1% Triton-X 100, 0.5 M EDTA 0.5 M, 2% sodium chloride 1M for 30 min on ice and the protein concentration was determined by Bradford assay by using a Jasco V-630 spectrophotometer. Lysates were prepared to examine the expression of Bax (N-20, sc-493, 1:100; Santa Cruz Biotechnology), Cyclin D1 (SR4, 1:1,000; Thermo Scientific), Cyclin E (M-20, sc-481, 1:500; Santa Cruz Biotechnology), VDR (1:750; Santa Cruz Biotechnology), E-cadherin (H-108, sc-7870, 1:500; Santa Cruz Biotechnology) and β-catenin (H-102, sc-7199, 1:1,000; Santa Cruz Biotechnology). The same amount of protein was separated by SDS-PAGE and transferred to PDVF membranes (Millipore). Membranes were subsequently blocked for 30 min at room temperature and further incubated at 4°C overnight with primary antibodies. Then, membranes were washed and incubated with the appropriate HRP-conjugated secondary antibodies (Santa Cruz Biotechnology) for 90 min at room temperature. Protein bands were visualized using the enhanced chemiluminescence method. Relative protein levels were calculated by normalization to the amount of actin protein (C-11, sc-1615, 1:1,000; Santa Cruz Biotechnology). Data shown are representative of three independent experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!