Dimedone

Whey proteins powder was kindly gifted by Asama Chemical Co. Ltd. (Tokyo, Japan). Dimedone and 2,4,6-Trinitrobenzenesulfonic acid (TNBS) were bought from Tokyo Chemical Industry (Tokyo, Japan), and Alexa 680 Fluor C2 Maleimide was from Thermo Scientific (Rockford, IL, USA). Anti-cysteine sulfenic acid antibody was obtained from Millipore (MA, USA). HRP- conjugated anti-rabbit or mouse IgG, anti-β-actin, and anti-caspase-3 antibodies were purchased from Cell Signaling, Inc. (Beverly, MA, USA). A blood urea nitrogen (BUN) kit was purchased from Thermo Fisher Scientific (Frederick, MD, USA). Anti-lipocalin-2 antibody was obtained from Adipogen (MI, USA). E-cadherin were bought from BD Biosciences, (NJ, USA). Cytokines IL-1α, IL-1β, and TNF-α assay kits were purchased from PeproTech (Rocky Hill, NJ, USA). ROS and superoxide were from Enzo life science (NY, USA), NOX-2 antibody was brought from Bioss Antibodies (MA, USA) and NOX-4 antibody was purchased from Novus Biologicals (CO, USA), H₂O₂ detection kit was from Cayman Chemical Company (MI, USA), Calcein-AM and Propidium Iodide (PI) Staining Kit was from Dojindo Molecular Technologies (MD, USA). Pentobarbital sodium was from Kyoritsu Seiyaku Corporation (Tokyo, Japan). Maleimide and all other chemicals were bought from Sigma-Aldrich (St. Louis, MO, USA).