Tris acetate edta buffer
Tris-acetate EDTA Buffer is a laboratory buffer solution used to maintain a specific pH range in various biochemical and molecular biology applications. It is a commonly used buffer in procedures such as gel electrophoresis, DNA and RNA purification, and enzymatic reactions. The buffer helps to stabilize the pH environment, which is crucial for the proper function and integrity of biomolecules.
Lab products found in correlation
9 protocols using tris acetate edta buffer
Agarose Gel Electrophoresis Protocol
Quantifying Poly(I:C) in Engineered Nanoparticles
For the release of poly(I:C), ENCPs were incubated in cell culture media, in a 1:1 (v/v) ratio, for 4 or 24 h, prior to been processed as described above. Free poly(I:C) exposed to the same conditions was used as the control.
Lateral Flow Biosensor for Multiplex Detection
α-Amp-short: 5′-SH-(C6H12)-CAC GGC ATG GTG GGC GTC GTG-3′
α-Amp-pol(T): 5′-SH-(C6H12)-TTT TTT TTT TTT TTT CAC GGC ATG GTG GGC GTC GTG-3′
α-CRP: 5′-SH-(C6H12)-ACA CGA TGG GGG GGT ATG ATT TGA TGT GGT TGT TGC ATG ATC GTG G-3′
Molecular Biology Toolkit: Reagents Inventory
Amplification of spa Gene Region
RFLP-based Genotypic Classification of PsHV
The resulting DNA fragments were separated by electrophoresis on a 0.5% agarose gel in 1× Tris–acetate–EDTA buffer (Sigma–Aldrich, St. Louis, MO, USA) containing 5 μL of ethidium bromide (10 mg/mL) (Life Technologies, Grand Island, NE, USA) at room temperature under constant voltage of 20 V for about 15 h. The BenchTop 1 kb DNA Ladder (Promega, Madison, WI, USA) was used as a DNA size marker. The gels were observed at 254 nm using a MacroVue UV transilluminator (GE Healthcare, Cleveland, OH, USA) and photographed.
PCR Screening of B. cereus Virulence Factors
For several B. cereus strains, the presence of Nhe and HBL toxins in the supernatant was also assessed using the immunodetection kit Duopath Cereus Enterotoxins (Merck, Darmstadt, Germany).
Molecular Detection of MRSA Isolates
Rapid DNA Extraction from Zebrafish Embryos
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