The Folin-Ciocalteu phenol reagent (FCR), gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), ferrozine, caffeic acid, quercetin, p-coumaric acid, ferulic acid, rutin, and kaempferol-3-O-rutinoside were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol, acetonitrile, sodium carbonate, ferric chloride, ferrous chloride, ferrous sulfate, and potassium persulfate were acquired from Avantor Performance Materials (Gliwice, Poland). The ACL kit was purchased from Analytik Jena (Jena, Germany).
Potassium persulfate
Manufactured by Avantor
Sourced in Poland, Germany, United States
Potassium persulfate is an inorganic chemical compound with the formula K2S2O8. It is a white crystalline solid that is soluble in water. Potassium persulfate is commonly used as an oxidizing agent, initiator, and polymerization catalyst in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Acetone, by Avantor (3 mentions)
Gallic acid, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Methanol, by Avantor (2 mentions)
Acetonitrile, by Avantor (2 mentions)
Sodium carbonate, by Avantor (2 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid abts, by Merck Group (2 mentions)
Ethanol, by Avantor (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
Potassium carbonate, by Thermo Fisher Scientific (2 mentions)
Dichloromethane, by Avantor (2 mentions)
Chloroform, by Avantor (2 mentions)
Potassium hydroxide, by Avantor (2 mentions)
Triallylamine, by Merck Group (2 mentions)
Sodium metabisulphite, by Avantor (2 mentions)
Sodium hydroxide (naoh), by Avantor (2 mentions)
Allyl bromide, by Thermo Fisher Scientific (2 mentions)
Diallyldimethylammonium chloride, by Merck Group (2 mentions)
1 3 bis 4 piperidyl propane, by Thermo Fisher Scientific (2 mentions)
N n methylenebisacrylamide, by Avantor (2 mentions)
9 protocols using potassium persulfate
1
Antioxidant Capacity Assays and Reagents
2
Antioxidant-rich Tomato Powder Production
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Tomato fruits (S. lycopersicum var. Saladette) were obtained from greenhouses located in Tulancingo, Hidalgo, Mexico. Spray-dried gum arabic (GA) (Cedrosa, S.A. de C.V., Naucalpan, Mexico, Mexico) and 28-32 DE maltodextrin (MD) (Reasol S.A. de C.V., CDMX, Mexico) were used as wall materials. Potassium persulfate, hexane, ethanol, and acetone were purchased from J.T. Baker S.A. de C.V. (Avantor Performance Materials, Ecatepec, Estado de México, Mexico). 2,2'-diphenyl-1-picrylhydrazyl (DPPH•), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), and ABTS [2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] were purchased from Sigma-Aldrich (Sigma-Aldrich Química S.A. de C.V., Toluca, Estado de México, Mexico).
3
Polymerization of Ionic Hydrogels
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Diallyldimethylammonium chloride (65 wt % in H2O) and triallylamine (99%) were purchased from Sigma Aldrich, and piperazine and D2O (99.9%) were obtained from Merck KGaA (Darmstadt, Germany). Allyl bromide (99%), potassium carbonate (99%), methanol (99%), and 1,3-bis(4-piperidyl)propane (97+%) were purchased from Alfa Aesar (Kandel, Germany). The anion exchange-resin Lewatit Monoplus MP 800 was provided by Lanxess (Leverkusen, Germany). Chloroform (≥99%), acetone (≥99%), bidistilled water, potassium hydroxide, dichloromethane (≥99%), potassium persulfate, sodium metabisulphite, N,N′-methylenebisacrylamide and sodium hydroxide (97%) were obtained from VWR International GmbH (Darmstadt, Germany). All chemicals were used as received. Polyester filter bags with a maximum mesh size of 90 µm were purchased from Rosin Tech Products (Bethpage, NY, USA).
4
Synthesis of Anion Exchange Resins
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The anion exchange-resin Lewatit Monoplus MP 800 was provided by Lanxess (Leverkusen, Germany). Double-distilled water, potassium hydroxide, chloroform (≥99%), acetone (≥99%), dichloromethane (≥99%), potassium persulfate, sodium metabisulphite, sodium hydroxide (97%) and N,N’-methylenebisacrylamide were obtained from VWR International GmbH (Darmstadt, Germany). Potassium carbonate (99%), allyl bromide (99%), methanol (99%) and 1,3-bis(4-piperidyl)propane (97+%) were obtained from Alfa Aesar (Kandel, Germany), diallyldimethylammonium chloride (65 wt% in H2O) and triallylamine (99%) were obtained from Sigma Aldrich. Piperazine, diallylamine (97%) and D2O (99.9%) were purchased from Merck KGaA (Darmstadt, Germany). The polyester filter bags with a maximum mesh size of 90 µm were bought from Rosin Tech Products (Bethpage, NY, USA).
5
Antioxidant Compound Sourcing and Characterization
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Caftaric acid, 2,2-azino-bis-3-ethylbenzthioazolin-6-sulfonic acid, Trolox, butylated hydroxytoluene, 2,2-diphenyl-1-picrylhydrazyl, fluorescein, 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH), disodium phosphate, and sodium chloride were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Potassium chloride and potassium persulfate were purchased from VWR (Fontenay-sous-Bois, France), and potassium dihydrogen phosphate was purchased from Merck (Darmstadt, Germany). Isoquercitrin, rutin, luteolin-7-O-glucoside, isorhamnetin-3-O-rutinoside, and apigenin-7-O-glucoside were purchased from Extrasynthese (Genay, France).
Ultrapure water (18.2 MΩ‧cm) was used for extraction and chromatography and was prepared using a Millipore Synergy system (Molsheim, France). Hydrochloric acid, acetonitrile (ACN), ethyl acetate, and ethanol (EtOH) were purchased from Fisher Scientific (Illkirch-Graffenstaden, France). Methanol (MeOH) and chloroform were obtained from Carlo Erba (Val-de-Reuil, France). Formic acid was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
Ultrapure water (18.2 MΩ‧cm) was used for extraction and chromatography and was prepared using a Millipore Synergy system (Molsheim, France). Hydrochloric acid, acetonitrile (ACN), ethyl acetate, and ethanol (EtOH) were purchased from Fisher Scientific (Illkirch-Graffenstaden, France). Methanol (MeOH) and chloroform were obtained from Carlo Erba (Val-de-Reuil, France). Formic acid was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France).
6
Evaluation of Antioxidant and Digestive Enzyme Inhibition Activities
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ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), Trolox (6-hydroxyl-2,5,7,8-tetramethylchroman-2-carboxylic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), TFA (trifluoroacetic acid), gallic acid, Folin–Ciocalteu reagent (FC), pepsin from porcine gastric mucosa-lyophilized powder, α-amylase and pancreatin from porcine pancreas, heat-stable α-amylase solution, protease, amyloglucosidase solution, 2-(N-Morpholino) ethanesulfonic acid (MES), and Tris (hydroxymethyl) aminomethane (TRIS) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Technical grades of methanol (MeOH) (>98.5%), ethanol (C2H5OH) (93%), sodium hydroxide (NaOH) (97%), sodium chloride (NaCl) (97%), potassium persulfate (K2S2O8) (100%), sodium carbonate (Na2CO3) (>99%), nitric acid (HNO3) (>99%), sulfuric acid (H2SO4) (>97%), hydrochloric acid (HCl) (37.2% w/w), petroleum ether (C6H14) (30–60 °C, A.R), Kjeldahl tablet (CuH10O9S), potassium chloride (KCl) (> 99%), sodium hydrogen carbonate (NaHCO3) (>99%), sodium chloride (NaCl) (97%), magnesium chloride (MgCl2) (99%), ammonium chloride (NH4Cl) (100%), calcium chloride (CaCl2) (>93%), monopotassium phosphate (KH2PO4), and indicators (tashiro and phenolphthalein) were purchased from VWR International (Leuven, Belgium). Inductively coupled plasma (ICP) multi-element standard solution IV was procured from Merck KGak (Darmstadt, Germany).
7
Comprehensive Polyphenol Extract Characterization
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SP4TM is a patent pending formulation (PCT/US2022/021087) composed by a mixture of plant extracts containing a high content of type-A proanthocyanidins and other polyphenols. The origin of the plants is included in the relevant patent (PCT/US2022/021087).
Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Thermo Fisher (Waltham, MA USA), cell lysis solution and luciferin were purchased from BPS Bioscience (San Diego, CA, USA), HepG2 human hepatocyte carcinoma, 85,011,430 cell lines, Fetal Calf Serum (FCS), ethanol (95%), Folin–Ciocolteu reagent, sodium carbonate, sodium nitrite, aluminum chloride, sodium hydroxide, potassium chloride, sodium acetate, potassium ferricyanide (99.8%), 1,1-diphenyl-2-picryl-hydrazil stable radical (DPPH•) (>98%), ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid) (98%), and phenolic compounds standards (including PAC-A2) were purchased from Sigma-Aldrich (Milan, Italy). The c-PAC standard was provided by Complete Phytochemical Solutions (Cambridge, USA). The 4-(dimethylamino)-cinnamaldehyde (DMAC), hydrochloric acid, acetic acid, acetone, acetonitrile, formic acid, potassium persulfate, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), and iron(III) chloride were purchased from VWR International (Radnor, PA, USA).
Dulbecco’s Modified Eagle Medium (DMEM) was purchased from Thermo Fisher (Waltham, MA USA), cell lysis solution and luciferin were purchased from BPS Bioscience (San Diego, CA, USA), HepG2 human hepatocyte carcinoma, 85,011,430 cell lines, Fetal Calf Serum (FCS), ethanol (95%), Folin–Ciocolteu reagent, sodium carbonate, sodium nitrite, aluminum chloride, sodium hydroxide, potassium chloride, sodium acetate, potassium ferricyanide (99.8%), 1,1-diphenyl-2-picryl-hydrazil stable radical (DPPH•) (>98%), ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid) (98%), and phenolic compounds standards (including PAC-A2) were purchased from Sigma-Aldrich (Milan, Italy). The c-PAC standard was provided by Complete Phytochemical Solutions (Cambridge, USA). The 4-(dimethylamino)-cinnamaldehyde (DMAC), hydrochloric acid, acetic acid, acetone, acetonitrile, formic acid, potassium persulfate, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), and iron(III) chloride were purchased from VWR International (Radnor, PA, USA).
8
DNA-Functionalized Surface Characterization
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Sodium acetate (NaAc; p.a., POCH, Poland), magnesium acetate (Mg(Ac)2; p.a., POCH, Poland), potassium persulfate (VWR Chemicals), potassium hydroxide (POCH, Poland), trisodium phosphate (Chempur, Poland), 1× PBS buffer (pH 7.4; Sigma), absolute ethanol (99.8%; POCH, Poland), EDTA (Sigma), tris–EDTA buffer (TE; Sigma), tris(2-carboxyethyl)phosphine hydrochloride (TCEP; Sigma), 2-amino-2-(hydroxymethyl)propane-1,3-diol (Tris; Sigma), 6-mercaptohexan-1-ol (MCH; Sigma) were all of high purity, and were used as received. For all the experiments, we used distilled and deionized water with a conductivity of 0.056 μS cm−1 produced by the Hydrolab system. The following oligonucleotides, purchased from MWG-Operon (Eurofins), were used:
• Capture DNA (5′ → 3′): thiol-C6-GCCTTCACAGGGTCCTTTATGT.
• Complementary target DNA (5′ → 3′): ACATAAAGGACCCTGTGAAGGC.
• Capture DNA (5′ → 3′): thiol-C6-GCCTTCACAGGGTCCTTTATGT.
• Complementary target DNA (5′ → 3′): ACATAAAGGACCCTGTGAAGGC.
9
Analytical Reagents Characterization Protocol
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Chemical reagents : Silver nitrate (AgNO3,
ACS reagent grade, ≥99.0%),
nitric acid (HNO3, ACS reagent grade, 68–70%), potassium
metaperiodate (KIO4, ACS reagent grade, ≥99.8%),
periodic acid (H5IO6, ACS reagent grade, 99%),
sodium carbonate (Na2CO3, 99.95–100.05%
ACS primary standard), potassium hydrogen phthalate (KHP, C8H5KO4, 99.99%, acidimetric standard), and acetone
((CH3)2CO, ACS reagent grade, 99.5%) were obtained
from Sigma-Aldrich, St. Louis, Missouri, United States; potassium
persulfate (K2S2O8, ACS reagent grade,
≥99.0%) was obtained from VWR, Mississauga, Ontario, Canada.
All reagents were used without further purification. Unless otherwise
mentioned, reverse osmosis (RO) water was used for all experimental
procedures.
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ACS reagent grade, ≥99.0%),
nitric acid (HNO3, ACS reagent grade, 68–70%), potassium
metaperiodate (KIO4, ACS reagent grade, ≥99.8%),
periodic acid (H5IO6, ACS reagent grade, 99%),
sodium carbonate (Na2CO3, 99.95–100.05%
ACS primary standard), potassium hydrogen phthalate (KHP, C8H5KO4, 99.99%, acidimetric standard), and acetone
((CH3)2CO, ACS reagent grade, 99.5%) were obtained
from Sigma-Aldrich, St. Louis, Missouri, United States; potassium
persulfate (K2S2O8, ACS reagent grade,
≥99.0%) was obtained from VWR, Mississauga, Ontario, Canada.
All reagents were used without further purification. Unless otherwise
mentioned, reverse osmosis (RO) water was used for all experimental
procedures.
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