Cucl2

A PVC waste document bag was obtained from the living garbage. Dimethylformide (DMF), dimethylsulfoxide (DMSO), thiourea, potassium hydroxide (KOH), hydrochloric acid (HCl), and various metal chlorides, including FeCl₂, CoCl₂, NiCl₂, CuCl₂, PbCl₂, or CdCl₂ were purchased from Beijing Chemical Works and Aladdin Chemistry Co., Ltd. All chemical reagents were used without further purification.

AD5 and cyclin D1 were purified as previously described (13 (link),21 ). A spectroscopic sample of AD5 was prepared in phosphate-buffered solution (PBS) at pH 7.4. The concentration of purified AD5 and cyclin D1 was determined using a Bradford assay (Sangon Biotech Co., Ltd., Shanghai, China), according to the manufacturer's protocol. The anti-V5-tag antibody (cat. no. M1008-2) was purchased from Hangzhou HuaAn Biotechnology Co., Ltd. (Hangzhou, China). Horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (cat. no. SA00001-1) was purchased from the ProteinTech Group, Inc. (Chicago, IL, USA). Bovine serum albumin (BSA) and o-phenylenediamine (OPD) were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). CuCl₂ and FeCl₃ were purchased from Beijing Chemical Works (Beijing, China). All other chemicals were of analytical grade.