N-Acetyl-D-penicillamine (NAP), sodium nitrite, L-cysteine, sodium chloride, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, copper (II) chloride, ethylenediaminetetraacetic acid (EDTA), and tetrahydrofuran (THF) were purchased from Sigma-Aldrich (St. Louis, MO). Methanol, hydrochloric acid, sulfuric acid, Luria Bertani (LB) broth and LB agar were obtained from Fisher Scientific (Hampton, NH). CarboSil 20 80A was obtained from DSM Biomedical Inc. (Berkeley, CA). All aqueous solutions were prepared with 18.2 MΩ-deionized water using a Milli-Q filter from EMD Millipore (Billerica, MA). Phosphate buffered saline (PBS), pH 7.4, containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, and 100 μM EDTA was used for all in vitro experiments. P. aeruginosa ATCC 27853 and P. mirabilis ATCC 29906 was obtained from the American Type Culture Collection (ATCC) (Manassas, VA).
Milli q filter
Manufactured by Merck Group
Sourced in United States, United Kingdom
The Milli-Q filter is a water purification system designed to produce high-quality, ultrapure water for laboratory and research applications. It utilizes a multi-stage filtration process to remove various impurities, including organic compounds, inorganic ions, and particulates, from the water supply.
Automatically generated - may contain errors
Lab products found in correlation
Sodium phosphate dibasic, by Merck Group (6 mentions)
Potassium phosphate monobasic, by Merck Group (6 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (5 mentions)
Sulfuric acid, by Thermo Fisher Scientific (5 mentions)
N acetyl d penicillamine, by Merck Group (4 mentions)
Methanol, by Thermo Fisher Scientific (4 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (4 mentions)
Sodium nitrite, by Merck Group (3 mentions)
Potassium chloride (kcl), by Merck Group (3 mentions)
L cysteine, by Merck Group (3 mentions)
N n dimethylacetamide dmac, by Merck Group (3 mentions)
Sulfuric acid, by Merck Group (3 mentions)
Hplc grade acetonitrile, by Merck Group (3 mentions)
Tetrahydrofuran, by Merck Group (3 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Tetrahydrofuran thf, by Merck Group (2 mentions)
P aeruginosa atcc 27853, by American Type Culture Collection (2 mentions)
Trypsin edta, by Corning (2 mentions)
Dulbecco s modification of eagle s medium dmem, by Corning (2 mentions)
Formic acid, by Merck Group (2 mentions)
17 protocols using milli q filter
1
Antimicrobial Efficacy of NAP-Based Compounds
2
Synthesis and Characterization of Antimicrobial Nanocomposites
3
Synthesis and Characterization of N-Acetyl-D-penicillamine Derivative
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N-Acetyl-D-penicillamine
(NAP), sodium nitrite,l -cysteine, sodium chloride, potassium
chloride, sodium phosphate dibasic, potassium phosphate monobasic,
copper(II) chloride, ethylenediaminetetraacetic acid (EDTA), tetrahydrofuran
(THF) and N,N-dimethylacetamide
(DMAc) were purchased from Sigma-Aldrich (St. Louis, MO). N-Acetyl-d ,l -penicillamine disulfide (NAP
disulfide) was obtained from Enzo Life Science, Inc. (New York, NY).
Methanol (MeOH), methyl ethyl ketone (MEK), hydrochloric acid, sulfuric
acid, Luria–Bertani (LB) broth and LB agar were products of
Fisher Scientific (Hampton, NH). CarboSil 20 80A was obtained from
DSM Biomedical Inc. (Berkeley, CA). An Agilent ZORBAX rapid resolution
high definition (RRHD) Eclipse Plus C18 column (2.1 × 50 mm,
1.8 μm particle size) was purchased from Altmann Analytik GmbH
& Co.KG (Munich, Germany). All aqueous solutions were prepared
with 18.2 MΩ-deionized water using a Milli-Q filter from EMD
Millipore (Billerica, MA). Phosphate buffered saline (PBS), pH 7.4,
containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, and 100
μM EDTA was used for all in vitro experiments. S. epidermidis ATCC 14990 and P. aeruginosa ATCC 27853 were obtained
from the American Type Culture Collection (ATCC) (Manassas, VA).
(NAP), sodium nitrite,
chloride, sodium phosphate dibasic, potassium phosphate monobasic,
copper(II) chloride, ethylenediaminetetraacetic acid (EDTA), tetrahydrofuran
(THF) and N,N-dimethylacetamide
(DMAc) were purchased from Sigma-Aldrich (St. Louis, MO). N-Acetyl-
disulfide) was obtained from Enzo Life Science, Inc. (New York, NY).
Methanol (MeOH), methyl ethyl ketone (MEK), hydrochloric acid, sulfuric
acid, Luria–Bertani (LB) broth and LB agar were products of
Fisher Scientific (Hampton, NH). CarboSil 20 80A was obtained from
DSM Biomedical Inc. (Berkeley, CA). An Agilent ZORBAX rapid resolution
high definition (RRHD) Eclipse Plus C18 column (2.1 × 50 mm,
1.8 μm particle size) was purchased from Altmann Analytik GmbH
& Co.KG (Munich, Germany). All aqueous solutions were prepared
with 18.2 MΩ-deionized water using a Milli-Q filter from EMD
Millipore (Billerica, MA). Phosphate buffered saline (PBS), pH 7.4,
containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, and 100
μM EDTA was used for all in vitro experiments. S. epidermidis ATCC 14990 and P. aeruginosa ATCC 27853 were obtained
from the American Type Culture Collection (ATCC) (Manassas, VA).
4
Nitric Oxide Release Measurement
5
NAP-Based Antimicrobial and Cytotoxicity Assay
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N-Acetyl-D-penicillamine (NAP), sodium chloride, copper chloride, L-cysteine, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, ethylenediaminetetraacetic acid (EDTA), tetrahydrofuran (THF), and sulfuric acid were purchased from Sigma-Aldrich (St. Louis, MO). Methanol, hydrochloric acid, silicone oil, and sulfuric acid were obtained from Fisher Scientific (Pittsburgh, PA). Saint-Gobain™ TygonTM Formula 3350 silicone rubber (SR) tubing was purchased from Fisher Scientific (Pittsburgh, PA). All aqueous solutions were prepared with 18.2 MΩ deionized water using a Milli-Q filter (Millipore Corp., Billerica, MA). Phosphate buffered saline (PBS), pH 7.4, containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 100 µM EDTA was used for all in vitro experiments. Trypsin -EDTA and Dulbecco’s modification of Eagle’s medium (DMEM) were obtained from Corning (Manassas, VA 20109). The antibiotic Penicillin-Streptomycin (Pen-Strep) and fetal bovine serum (FBS) were purchased from Gibco-Life Technologies (Grand Island NY 14072). The Cell Counting Kit -8 (CCK-8) was obtained from Sigma-Aldrich (St Louis MO 63103). The bacterial strains of Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 6538) and 3T3 mouse fibroblast cell line (ATCC 1658)were originally obtained from American Type Culture Collection (ATCC).
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N-Acetyl-D-penicillamine (NAP), sodium chloride, copper chloride, L-cysteine, potassium chloride, sodium phosphate dibasic, potassium phosphate monobasic, ethylenediaminetetraacetic acid (EDTA), tetrahydrofuran (THF), and sulfuric acid were purchased from Sigma-Aldrich (St. Louis, MO). Methanol, hydrochloric acid, silicone oil, and sulfuric acid were obtained from Fisher Scientific (Pittsburgh, PA). Saint-Gobain™ TygonTM Formula 3350 silicone rubber (SR) tubing was purchased from Fisher Scientific (Pittsburgh, PA). All aqueous solutions were prepared with 18.2 MΩ deionized water using a Milli-Q filter (Millipore Corp., Billerica, MA). Phosphate buffered saline (PBS), pH 7.4, containing 138 mM NaCl, 2.7 mM KCl, 10 mM sodium phosphate, 100 µM EDTA was used for all in vitro experiments. Trypsin -EDTA and Dulbecco’s modification of Eagle’s medium (DMEM) were obtained from Corning (Manassas, VA 20109). The antibiotic Penicillin-Streptomycin (Pen-Strep) and fetal bovine serum (FBS) were purchased from Gibco-Life Technologies (Grand Island NY 14072). The Cell Counting Kit -8 (CCK-8) was obtained from Sigma-Aldrich (St Louis MO 63103). The bacterial strains of Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 6538) and 3T3 mouse fibroblast cell line (ATCC 1658)were originally obtained from American Type Culture Collection (ATCC).
6
Lipid Extraction and Analysis Protocol
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PMA,LPS, and formic acid were purchased from Sigma-Aldrich. Mouse IL-4, human IL-4, and human IL-13 were obtained from PeproTech. High-performance liquid chromatography (HPLC)-grade chloroform, methanol, isopropanol (IPA), and hexane were used without further purification. Phosphoricacid, potassium chloride, and ammonium acetate were obtained from Sinopharm Chemical Reagent Co., Ltd. The water was purified using a 0.22-µm Milli-Qfilter (Millipore, USA).
7
Analytical Standards for Phytochemical Research
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Analytical grade solvents (acetone, dichloromethane, ethanol, ethyl acetate, hexane and methanol) and HPLC-grade methanol and acetonitrile were purchased from Tedia (Fairfield, USA). Water was processed by Milli-Q filter (Millipore Corporation, Billerica, USA). Phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharide (LPS) were from Sigma-Aldrich (USA), dexamethasone, dimethyl sulfoxide (DMSO) were from Sigma-Aldrich (USA), Merck (USA), Hospira (Australia) and MP Biomedical Inc. (USA) respectively. Chemical standards for artemetin, casticin and vitexilactone were from ChemFaces (China), while α-amyrin, β-amyrin, butylated hydroxytoluene (BHT), campesterol, 2,4-Di-tert-butylphenol, maslinic acid, phytol, β-sitosterol, and stigmasterol were from Sigma-Aldrich (USA).
8
Quantitation of SF, DC, and Ledipasvir in Plasma
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Drug standards for SF, DC, and Ledipasvir (IS) were kindly supplied by Memphis Co. for Pharmaceutical and Chemical Industries, Cairo, Egypt, with certified purity of 99.98 ± 0.421 for SF, 99.93 ± 0.231 for DC, and 99.87 ± 0.642 for Ledipasvir. Darvoni film-coated tablets (coformualted with 400 mg SF and 60 mg DC) were purchased from Beacon Pharmaceuticals Limited, Bangladesh. Drug-free human plasma was obtained from Kuwait Blood Bank, Al Jabriyah, Kuwait. HPLC grade acetonitrile and other used chemicals in the adopted method were of analytical grade and obtained from Sigma Aldrich, Dor-set, UK. “In house” HPLC grade water was prepared with a MilliQfilter purchased from Millipore, Watford, UK. Syringe membrane filters (13mm) were purchased from kinesis scientific expert, Cambridgeshire, UK. Nylon solvent filters (0.45 um) used for solvent filtration and Water 20-positions Extraction Manifold with SPE cartridges (Sep-Pak® Vac C18) used for sample preparation were purchased from Water Corporation, Milford, USA. SPE eluates were dried using DRI-BLOCK DB-3 evaporator which was purchased from Techne, Stone, UK.
9
Staphylococcus aureus Antimicrobial Assay
10
Analytical-grade Catechol Quantification
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All solutions and electrolytes were of analytical grade (Vetec Química Fina Ltd. (Rio de Janeiro, Brasil)). The water used was purified with a Millipore Milli-Q filter (Millipore S/A, (Molsheim, França)) and had an electrical conductivity of ≤0.1 µS cm−1. The catechol standard was acquired from Sigma-Aldrich (St. Louis, MO, USA). All standard solutions were prepared by dilution of 1 mM stock solutions, leading to a final concentration of 100 µM.
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