Our swept-source OCT system (Figure 1 A) is based on a swept source (HSL-2000, Santec Corp., Japan) with a 1310 nm center wavelength, 110 nm full width at half maximum bandwidth, and 20 kHz scan rate. The input light is split by a 50-50 fiber coupler and then directed to a reference arm and a sample arm by two circulators. Fiber polarization is controlled by two manual fiber polarization controllers. The reference arm is attenuated by a variable density filter. A galvo mirror system (GVS002, Thorlabs) is used to scan the sample arm light beam. The interference signal was detected by a balanced detector (PDB450C, Thorlabs) and sent to a data acquisition board (ATS9462, Alazartec Technologies Inc). Real-time OCT B-scan images are displayed on the monitor. The lateral resolution of the system in air was 10 µm, and the axial resolution was 6 µm by the FWHM definition.
Pdb450c
Manufactured by Thorlabs
The PDB450C is a photodiode detector module produced by Thorlabs. It features a high-speed silicon photodiode, a transimpedance amplifier, and integrated power supply circuitry.
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Lab products found in correlation
6 protocols using pdb450c
1
Swept-Source OCT Imaging System
2
Fiber-based Particle Position Detection
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Fiber-based position detection of trapped particles can be used to calibrate the spring constant of the optical trap. According to our previous work36 (link), the differential signals (scattering light) collected by two fibers in AFMOTs scale with y-displacement (Fig. 1 c) relative to the trap center when the motion of the trapped particle is small (< 100 nm). Once a particle is trapped, no objective lens or microscope is required to detect the particle position. In the experiment, the back-scattered light by the trapped particle was collected by the two prefixed optical fibers and measured by two inputs (PD1 and PD2) of a balanced photodiode (PDB450C, THORLABS). AFMOTs can achieve 2 nm spatial resolution and hundreds of MHz bandwidth.
3
High-Resolution Swept-Source OCT Imaging
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Fiber-Based Position Detection in Optical Tweezers
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The fiber based position detection mechanism on the inclined fiber optical tweezers platform is designed based on the dual fiber optical tweezers, which can be found in our previous work38 (link),41 ,42 (link). Briefly, the fiber based position detection mechanism, as well as the inclined fiber optical tweezers, was set up on a microscope platform. Light from a fiber-coupled 974 nm laser diode (AC 1405-0400-0974-SM-500, Eques) was split into two lensed fibers (TLF SM1060, Nanonics Imaging) through a 3dB coupler (22-12798-50-23162, GouldFiber Optics), as shown in Fig. 1a . All the fibers in the system were single-mode at 974 nm. The two light beams emitted from the lensed fiber tips can three-dimensionally trap microscale particles in water close to the beam intersection. The back-scattered light by the bead was collected by the two lensed optical fibers and measured by two inputs (PD1 and PD2) of a balanced photodiode (PDB450C, THORLABS). The differential output from the photodiode enabled the measurements of bead positions with nanometer resolution. The forward-scattered light collected by an objective lens was recorded by either a PSD (DL100-7-PCBA3, First Sensor) or a high-speed camera (iXon 3 EMCCD, Andor).
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Swept-Source Optical Coherence Tomography System
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The SS-OCT system (details see Supplementary Material) is based on a swept source (HSL-2000, Santec Corp., Japan) with a 1310 nm center wavelength, 110 nm full width at half maximum bandwidth, and 20 kHz scan rate. The interference signal was detected by a balanced detector (Thorlabs PDB450C) and sent to a data acquisition board (ATS9462, Alazartec Technologies Inc). The lateral resolution of the system in air was 10 μm, and the axial resolution was 6 μm. To balance the effects of system signal-to-noise ratio roll-off and Gaussian beam focusing, we performed a calibration test by measuring attenuated mirror signals from different imaging depths.
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High-Resolution Swept-Source OCT Imaging
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