Plant samples (0.5 g) were treated with a 4:1 nitric acid: perchloric acid solution (v:v) for 12 h, digested to a clear solution, filtered, and diluted to a volume of 50 mL. The Cd content was then determined using an iCAP 6300 ICP spectrometer (Thermo Scientific, Waltham, MA, USA) (Tang et al., 2020 (link)). The translocation factor (TF) was calculated as the Cd content of shoots divided by the Cd content of roots (Rastmanesh et al., 2010 (link)).
Icap 6300 icp spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ICAP 6300 ICP spectrometer is an inductively coupled plasma optical emission spectrometer (ICP-OES) designed for elemental analysis. It utilizes a plasma source to atomize and ionize samples, and an optical system to detect and measure the intensity of specific wavelengths emitted by the excited elements. The ICAP 6300 can be used to quantify a wide range of elements in various sample matrices.
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4 protocols using icap 6300 icp spectrometer
1
Quantifying Cadmium in Plant Samples
2
Characterization of Transition Metal Oxide Nanoparticles
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Pt NPs, Pd NPs, PdCu nano-alloy, Ag-Cu2O nanocomposite, Ag, Cu2O, transition metal oxides (TMO) NPs and the catalyst samples were characterized by powder X-ray diffraction (Bruker D8-advance X-ray powder diffractometer (Cu Kα, λ=1.5406 Å)), transmission electron microscope (TEM, Hitachi model H-800), HRTEM (recorded by a FEI Tecnai G2 F20 S-Twin high-resolution transmission electron microscope working at 200 kV and a FEI Titan 80–300 transmission electron microscope equipped with a spherical aberration (Cs) corrector for the objective lens working at 300 kV), and scanning electron microscope (JSM-6301F). XPS were recorded on a VG EscaLab 220i-XL spectrometer using a standard Al Kα X-ray source (300 W) and an analyzer pass energy of 20 eV. All binding energies were referenced to the adventitious C1s line at
284.9 eV. Specific surface area was determined from N2 adsorption isotherm at −196 °C using standard Brunauer–Emmett–Teller (BET) theory. Before the measurement of N2 adsorption, degassing was conducted at 300 °C for 4 h. The catalyst loadings were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) on a Thermo Scientific iCAP 6300 ICP spectrometer.
284.9 eV. Specific surface area was determined from N2 adsorption isotherm at −196 °C using standard Brunauer–Emmett–Teller (BET) theory. Before the measurement of N2 adsorption, degassing was conducted at 300 °C for 4 h. The catalyst loadings were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES) on a Thermo Scientific iCAP 6300 ICP spectrometer.
3
Determination of Berry Potassium Content
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The berry skin and pulp samples of the 42nd days after the first application of potassium-containing fertilizers were used to determine the total potassium content with three biological repetitions. The samples were dried using an oven. The finely ground dry samples were digested with sulfuric acid and hydrogen peroxide (5:1, v/v), and the digestion solution was used to determine the total potassium content using an ICAP6300 ICP spectrometer (Thermo Scientific, Waltham, MA, USA) [58 ].
4
Phytoremediation Assessment of Nasturtium officinale
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Thirty days after the first SA spray (October 2020), the fourth and fifth mature leaves of N. officinale were collected to determine the photosynthetic pigment (chlorophyll a, chlorophyll b, and carotenoid) contents, antioxidant enzyme [peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)] activities, and soluble protein content as reported by Hao et al. (2004) ; Lin et al. (2020) (link)and Tang et al. (2022) (link) and . After that, the plants were uprooted, cleaned, and dried as described by Lin et al. (2020) (link). The root and shoot (dry weight) were weighed using an electronic balance. The dried samples were finely ground and digested (Lin et al., 2020 (link)), and the root and shoot Cd contents were determined using an ICAP6300 ICP spectrometer (Thermo Scientific, Waltham, MA, USA). The root Cd extraction and the shoot Cd extraction were calculated as follows: root Cd extraction = root Cd content × root biomass, and shoot Cd extraction = shoot Cd content × shoot biomass (Zhang et al., 2010 (link)). Finally, the pot soil was collected, air-dried, and sieved to determine the soil pH and exchangeable Cd concentration using a pH meter and an ICAP6300 ICP spectrometer, respectively (Bao, 2000 ; Tang et al., 2022 (link)).
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