A vascular catheter draining into the left uterine artery was placed in timed pregnant Hsd:Sprague Dawley SD rats (Envigo, Indianapolis, IN) as described (Yao et al. 2010 (link)) , including placement of ligatures on the iliac, superior gluteal and hypogastric trunk arteries. Catheters were secured within a Covance Infusion Harness (CIH95, Instech Lab, Plymouth Meeting PA) and attached to a single channel stainless steel swivel (375/22, Instech Lab, Plymouth Meeting PA). Glucose was infused through the catheter at the rate of 4 mg/min for 48 hours during late gestation to expose the left uterine horn fetuses to hyperglycemia. During infusion, the pregnant dams were awake and mobile, with unlimited access to water and food. For studies examining fetal outcomes, infusions were conducted on gestational days (GD) 18–20 in order to allow examination on GD20, prior to the time of parturition on GD21. For studies examining offspring, infusions were conducted on GD 19–21, to allow C-section delivery on GD21. Fetuses delivered by C-section were cross-fostered to healthy mothers.
Hsd sprague dawley sd rats
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The Hsd:Sprague Dawley SD rats are a commonly used laboratory animal model. They are a strain of albino rats bred for research purposes.
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2 protocols using hsd sprague dawley sd rats
1
Maternal Hyperglycemia in Pregnant Rats
2
Rat Husbandry and Euthanasia Protocol
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Male and female Hsd:Sprague-Dawley® (SD®) rats (age 12–15 wk) were purchased from Envigo. Animals were housed in the NIEHS Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)–approved animal care facility [ humidity, room temperature, 12-h light/dark cycle, polycarbonate shoebox cages (Tecniplast), and Sani-Chip® bedding (PJ Murphy Forest Products)] for at least 1 wk prior to use and allowed access to food (NIH #31) and tap water ad libitum consumption. Animals were euthanized by inhalation followed by decapitation. All animal protocols were approved by the Animal Care and Use Committee at the NIEHS according to the guidelines from the NIH. All data are reported in compliance with the Animal Research Reporting In Vivo Experiments (ARRIVE) guidelines.
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