All sections were dried, rehydrated in phosphate buffered saline (PBS), and rinsed in PBS. Sections were blocked for 60 min in 5% BSA (PBS containing 5% BSA and 0.2% Triton X-100; Sigma) and incubated with the appropriate primary antibodies, either mouse monoclonal anti-NeuN antibody (Millpore; 1:500, NeuN is a neuronal marker)/ anti-p53 antibody (GeneTex ; 1:500) / anti-annexin V antibody (Abcam ; 1:500) / anti-p-p53 antibody (Cell signaling ; 1:1000) / or anti-PUMA antibody (Abcam ; 1:200) at 4°C overnight and with secondary antibodies (Alexa Fluor® 488 goat anti-rabbit IgG (1:200, Jackson ImmunoResearch, West Grove, PA); Alexa Fluor® 594 anti-mouse IgG (1:200 dilution, Jackson ImmunoResearch, West Grove, PA)) at room temperature for 2 h. Sections were mounted with Mounting Medium H-1000 (Vector Laboratories, Burlingame, CA, USA). The numbers of NeuN-, p53-, annexin V-, p-p53-, and PUMA–positive cells were counted in 5 randomly selected fields by means of SPOT image analysis software (Diagnostic Instruments, Sterling Heights, MI)
Anti p p53 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-p-p53 antibody is a laboratory reagent designed for the detection and analysis of phosphorylated p53 protein. It can be used in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunocytochemistry, to study the activation and regulation of the p53 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Anti p53 antibody, by GeneTex (1 mentions)
Anti annexin 5 antibody, by Abcam (1 mentions)
Anti puma antibody, by Abcam (1 mentions)
Alexa fluor 594 anti mouse igg, by Jackson ImmunoResearch (1 mentions)
H 1000 mounting medium, by Vector Laboratories (1 mentions)
Anti p p38 antibody, by Cell Signaling Technology (1 mentions)
Anti p38 antibody, by Cell Signaling Technology (1 mentions)
Anti p53 antibody, by Cell Signaling Technology (1 mentions)
2 protocols using anti p p53 antibody
1
Immunofluorescence Quantification of Neuronal Markers
2
Protein Expression Analysis in Cell Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cells in each group were harvested for protein extraction. Protein samples (20 µl) were resolved on a 10–12% sodium dodecyl sulfate (SDS)-polyacrylamide gel. Then the protein was transferred onto polyvinylidene difluoride (PVDF) or nitrocellulose membranes, blocked in 5% fresh nonfat dry milk for 2 h, and probed with the following primary antibodies overnight at 4°C: anti-Wip1 antibody(2380-MC-100; R&D Systems), anti-p38 antibody (Cell Signaling Technology), anti-p-p38 antibody (Cell Signaling Technology), anti-p53 antibody (Cell Signaling Technology), or anti-p-p53 antibody (Cell Signaling Technology). The membranes were then incubated with the appropriate horseradish peroxidase-conjugated secondary antibodies for 2 h at room temperature. Enhanced chemiluminescence and densitometric analysis were finally analyzed.
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