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Donkey anti rabbit igg alexa488

Manufactured by Jackson ImmunoResearch
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Donkey anti-Rabbit IgG Alexa488 is a secondary antibody conjugated with the Alexa Fluor 488 fluorescent dye. It is designed to detect and bind to rabbit immunoglobulin G (IgG) antibodies.

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Lab products found in correlation

Cell tracker violet, by Thermo Fisher Scientific (2 mentions) Dylight antibody labeling kit, by Thermo Fisher Scientific (2 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (2 mentions) Dnase 1 from bovine pancreas, by Merck Group (2 mentions) Donkey anti rat igg dylight488, by Jackson ImmunoResearch (2 mentions) Anti ter 119 percp cy5.5 ter 119, by Thermo Fisher Scientific (2 mentions) Anti gfp fm264g, by BioLegend (2 mentions) Anti mouse parm1 epr10009, by Abcam (2 mentions) Donkey anti goat igg dylight488, by Jackson ImmunoResearch (2 mentions) Goat f ab 2 anti human igg pe, by Thermo Fisher Scientific (2 mentions) Fitc labeled sambucus nigra sna lectin, by Vector Laboratories (2 mentions) Polyclonal goat anti mouse nrp1 and mouse cd22 fc fusion proteins, by R&D Systems (2 mentions) Cy3 donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Donkey anti mouse igg alexa 488, by Jackson ImmunoResearch (1 mentions) Ib4 fitc, by Merck Group (1 mentions) Mab5406, by Merck Group (1 mentions) Ab2251 1, by Merck Group (1 mentions) Ab134054, by Abcam (1 mentions) Rat anti cd68 ab, by Bio-Rad (1 mentions) Goat anti rat igg rhodamine, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Donkey anti-rabbit Alexa488 (15 citations) Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

4 protocols using donkey anti rabbit igg alexa488

1

Immunofluorescent Staining Protocol for Neuronal Markers

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Immunofluorescent staining was performed as described previously (Gao et al., 2019 (link)). Tissue sections were blocked for 1 hr at room temperature in PBST (0.3% Triton X-100) with 5% normal donkey serum, followed by incubation with primary antibodies at 4°C overnight and secondary antibodies at room temperature for 2 hr in PBST (0.3% Triton X-100) with 1% normal donkey serum. Primary antibodies used for immunofluorescent staining were anti-MOR (rabbit, 1:500 for brain and spinal cord, 1:1000 for DRG, ABCAM, ab134054), anti-CGRP (goat, 1:500, ABCAM, ab36001), FITC-IB4 (1:200, Sigma, L2895), anti-c-Fos (rabbit, 1:15000, Synaptic System, 226003), anti-VGluT2 (guinea pig, 1:2000, Millipore, AB2251-I), anti-GAD67 (mouse, 1:500, Millipore, MAB5406). Secondary antibodies were donkey anti-goat IgG-Cy5, donkey anti-rabbit IgG-Cy3, donkey anti-rabbit IgG-Alexa 488, donkey anti-mouse IgG-Alexa 488 and donkey anti-guinea pig IgG-Cy5 (1:200, Jackson ImmunoResearch Laboratories).
Zhang X.Y., Dou Y.N., Yuan L., Li Q., Zhu Y.J., Wang M, & Sun Y.G. (2020). Different neuronal populations mediate inflammatory pain analgesia by exogenous and endogenous opioids. eLife, 9, e55289.
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2

Multiparameter Immune Cell Phenotyping

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Anti-CD31 PE-Cy7 (390), anti-CD45 PerCP-Cy5.5 (30-F11), anti-Gp38 PE (eBio8.1.1), and anti-Ter-119 PerCP-Cy5.5 (TER-119) were purchased from eBioscience. Anti-CD3 PE-Cy7 (145-2C11), anti-CD19 APC-Cy7 (6D5), anti-IgD APC (11.26c.2a), anti-CD326 PerCP-Cy5.5 (G8.8), anti-CD11a PerCP-Cy5.5, (H155-78), and anti-GFP (FM264G) were purchased from Biolegend. Anti-mouse Parm1 (EPR10009) was purchased from Abcam. Donkey anti-Rat IgG Dylight488, donkey anti-goat IgG Dylight488, and donkey anti-Rabbit IgG Alexa488 were purchased from Jackson ImmunoResearch Laboratories, Inc. HECA-452, MECA-79, MECA-367, MECA-99 were produced in our lab from hybridomas and labeled using DyLight Antibody Labeling Kit (Thermo Fisher Scientific) with fluorophores indicated. Goat F(ab)2 anti-human IgG PE, Carboxyfluorescein succinimidyl ester (CFSE) and Celltracker Violet were purchased from Invitrogen. Collagenase P and Dispase II, neutral protease, grade II were purchased from Roche. DNase I from bovine pancreas was purchased from Sigma. FITC labeled Sambucus Nigra (SNA) lectin was purchased from Vector laboratories. Polyclonal goat anti-mouse Nrp1 and mouse CD22-Fc fusion proteins were purchased from R&D systems. All reagents were titered or used according to the manufacturers’ recommendations. Antibodies used for immunoprecipitation and immunoblotting are described below.
Lee M., Kiefel H., LaJevic M.D., Macauley M.S., Kawashima H., O'Hara E., Pan J., Paulson J.C, & Butcher E.C. (2014). Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nature immunology, 15(10), 982-995.
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3

Multiparameter Immune Cell Phenotyping

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Anti-CD31 PE-Cy7 (390), anti-CD45 PerCP-Cy5.5 (30-F11), anti-Gp38 PE (eBio8.1.1), and anti-Ter-119 PerCP-Cy5.5 (TER-119) were purchased from eBioscience. Anti-CD3 PE-Cy7 (145-2C11), anti-CD19 APC-Cy7 (6D5), anti-IgD APC (11.26c.2a), anti-CD326 PerCP-Cy5.5 (G8.8), anti-CD11a PerCP-Cy5.5, (H155-78), and anti-GFP (FM264G) were purchased from Biolegend. Anti-mouse Parm1 (EPR10009) was purchased from Abcam. Donkey anti-Rat IgG Dylight488, donkey anti-goat IgG Dylight488, and donkey anti-Rabbit IgG Alexa488 were purchased from Jackson ImmunoResearch Laboratories, Inc. HECA-452, MECA-79, MECA-367, MECA-99 were produced in our lab from hybridomas and labeled using DyLight Antibody Labeling Kit (Thermo Fisher Scientific) with fluorophores indicated. Goat F(ab)2 anti-human IgG PE, Carboxyfluorescein succinimidyl ester (CFSE) and Celltracker Violet were purchased from Invitrogen. Collagenase P and Dispase II, neutral protease, grade II were purchased from Roche. DNase I from bovine pancreas was purchased from Sigma. FITC labeled Sambucus Nigra (SNA) lectin was purchased from Vector laboratories. Polyclonal goat anti-mouse Nrp1 and mouse CD22-Fc fusion proteins were purchased from R&D systems. All reagents were titered or used according to the manufacturers’ recommendations. Antibodies used for immunoprecipitation and immunoblotting are described below.
Lee M., Kiefel H., LaJevic M.D., Macauley M.S., Kawashima H., O'Hara E., Pan J., Paulson J.C, & Butcher E.C. (2014). Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nature immunology, 15(10), 982-995.
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4

Detailed Protocol for Experimental Autoimmune Encephalomyelitis

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MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) was purchased from Protein and Nucleic Acid Core Facility Stanford University, Stanford, CA, USA. Pertussis toxin was purchased from List Biologicals, St. Louis, MO, USA. Methocel was purchased from Sigma Aldrich, St. Louis, MO, USA. Rabbit anti-MBP Ab, rat anti-neurofilament Ab, rabbit Anti-Ym-1 Ab, rabbit anti-iNOS Ab for immunohistology were purchased from Abcam, Cambridge, MA, USA. Rat anti-CD68 Ab was purchased from Biorad, Hercules, CA, USA. Mouse anti-GFAP-Alexa fluor 488 Ab was purchased from Biolegend, San Diego, CA, USA. Rat anti-C3 Ab was purchased from Novus Biologicals, Littleton, CO, USA.
Donkey anti-rabbit IgG Alexa 488, goat anti-rat IgG Rhodamine, goat anti-rabbit IgG Rhodamine, and chicken anti-rat IgG Alexa488 were purchased from Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA. Antibodies against F4/80, iNOS, and CD206 for flow cytometry and ELISA kits for IFN-γ, IL-17A, GM-CSF and IL-10 were purchased from eBioscience, San Diego, CA, USA.
Yadav S.K., Ito N., Soin D., Ito K, & Dhib-Jalbut S. (2021). Dimethyl Fumarate Suppresses Demyelination and Axonal Loss through Reduction in Pro-Inflammatory Macrophage-Induced Reactive Astrocytes and Complement C3 Deposition. Journal of Clinical Medicine, 10(4), 857.
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